Aktivitas Antibakteri Metabolit Sekunder dari Ekstrak Metanol Biji Alpukat (Persea americana Mill)

Abstract

AbstrakMetabolit sekunder yang memiliki aktivitas antibakteri telah diisolasi dari ekstrak metanol biji alpukat (Parsea americana Mill). Proses isolasi diawali dengan ekstraksi menggunakan metanol, dilanjutkan dengan fraksinasi dan pemisahan menggunakan berbagai teknik kromatografi seperti kromatografi cair vakum (KCV) dan kromatografi kolom gravitasi (KKG). Dari proses isolasi tersebut diperoleh komponen K-1 yang berwujud padat dan berwarna putih. Komponen K-1 mempunyai titik lebur 67-70°C, larut dalam kloroform, etil asetat, dan etanol. Komponen K-1 mempunyai λmaks pada 219,0 nm mengindikasikan adanya transisi π→π* dan 344,0 nm mengindikasikan adanya transisi nπ*. Berdasarkan hasil interpretasi spektrum IR, komponen ini mengandung gugus -OH, gugus -C=O ester, ikatan -CH, dan ikatan C=C. Komponen K-1 diduga golongan saponin. Uji aktivitas antibakteri terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus mengindikasikan ekstrak metanol biji alpukat dan komponen K-1 aktif sebagai antibakteri, sehingga kedepannya memiliki prospek bagi pengembangan obat tradisional. Kata-kata kunci: biji alpukat (Parsea americana Mill), ekstrak metanol, aktivitas antibakteri AbstractAn antibacterial activity of secondary metabolite has been isolated from methanol extract of avocado seed (Parsea americana Mill). The isolation process has started with extracting using methanol, then fractioning and purifying by chromatography technique such as vacuum liquid chromatography and gravitation column chromatography. The white solid of K-1 component has been isolated from this process. The melting point of K-1 component was 67-70˚C and dissolved in chloroform, ethyl acetate, and ethanol. K-1 component has λmaks at 219,0 nm (π→π* transition) and 344,0 nm (n→π* transition). Based on IR spectrum interpretation, this component contained -OH group, -C=O group of ester, -CH bond, and C=C bond. K-1 component was predicted as saponin group. The antibacterial activity assay against Escherichia coli and Staphylococcus aureus indicated that methanol extract of avocado seed and K-1 component showed antibacterial activity toward both bacteria, so that this component has future chances for traditional medicine development.

Community Service Based on A Training Method for Making Liquid Hand Sanitizers to Prevent The Spread of COVID-19 in Tegalsari Kulon Progo

“…This is evidenced by its inhibitory effect on the growth of S aureus bacteria, which reached 72.45% for 30 seconds [5]. One ingredient for cleaning hands besides soap that can inhibit the growth of bacteria and even kill them is hand sanitizer [6]. Hand sanitizers are grouped in gel and liquid forms.…”

The mice’s hematological effect of given the Staphylococcus aureus and Persea americana

“…[13][14][15] Secondary metabolite components such as saponins, alkaloids, tannins, triterpenoids, and flavonoids, phytosterols, furanoic acid, oleic acid and linoleic acid, oxalic and phytic with varying proportions in avocado seeds, and other phytochemical substances play a role in these activities. [16][17][18] The purpose of this study was to observe the blood dynamics of mice (Mus musculus domesticus) which were infected with Staphylococcus aureus and then induced extracts of Persea americana, Mill.…”