AKTIVITAS ANTIBAKTERI EKSTRAK BUAH ADAS (Foeniculum vulgare, Mill)     PADA Vibrio harveyi DAN Vibrio alginolyticus Antibacterial Activity of Fennel (Foeniculum vulgare Mill) Extract on Vibrio alginolyticus and Vibrio harveyi

Budianto, Budianto; Prajitno, Arief; Yuniarti, Ating

doi:10.22146/agritech.9336

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…The increase in absorbance value after a dose of 100 mg/L indicates that there is the bacterial resistance to puring leaf extract because at a dose of 100 mg/L it has shown the first lowest value or the best from other doses. The minimum inhibitory concentration is the lowest concentration of antibiotics that will inhibit the growth of microscopic microorganisms (Budianto et al, 2015). From the data above, croton leaf extract (C. variegatum) with a concentration of 100 mg/L was chosen as the reference for the minimum dose to determine the concentration of the disc test.…”

Section: Results and Discussion Minimum Inhibitory Concentration (Mic Test)mentioning

confidence: 99%

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The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro

Fauziyyah

Prajitno

Fadjar

et al. 2021

JAFH

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Production problems related to aquaculture cannot be separated from diseases that can cause death in fish. Efforts to overcome the bacterial disease Edwardsiella tarda can be done by using natural materials such as the leaves of croton (Codiaeum variegatum). This study aimed to analyze the content of active compounds contained in croton leaf extract which is antibacterial against E. tarda bacteria. Inhibition test was performed by dilution method Minimum Inhibition Concentration (MIC test) and agar diffusion (disc test). The MIC test used five concentrations: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L, and 1000 mg/L on Tryptone Soy Broth (TSB) media, while the disc test used five doses of croton leaf extract, namely: A (100 mg/L), B ( 200 mg/L), C (300 mg/L), D (400 mg/L) and E (500 mg/L), 2 controls (positive and negative), and three replications on Tryptone Soy Agar (TSA) media and incubated for 2x24 h. The positive control used chloramphenicol (5 mg/L) antibiotic. Croton leaf extract contains several compounds, namely saponins, flavonoids, tannins, steroids, and polyphenols, which are antibacterial and could kill the test bacteria with the highest average inhibition zone results obtained at a dose of E (500 mg/L) of 6.34 mm, while the lowest results were obtained at dose A (100 mg/L) of 3.12 mm.

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Section: Results and Discussion Minimum Inhibitory Concentration (Mic Test)mentioning

confidence: 99%

“…Bacterial culture procedure according to Budianto et al (2015), the first thing to do is to prepare 5 ml of TSA media and put it in a test tube. The media was then sterilized in an autoclave for 15 minutes at a temperature of 121 0 C and a pressure of 1 atm.…”

Section: Research Materialsmentioning

confidence: 99%

The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro

Fauziyyah

Prajitno

Fadjar

et al. 2021

JAFH

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“…Extract preparation refers to the method used by Budianto et al (2015), with slight modifications. The bitter leaves were collected and cleaned of dirt that could not be seen with the naked eye and washed with running water until clean, and drained.…”

Section: Work Procedures Preparation Of Bitter Leaf Extractmentioning

confidence: 99%

Effect of Bitter Leaf Extract (Andrographis paniculata) against Edwardsiella tarda Bacteria In vitro

Pratama,

Prajitno,

Sulistiyati

et al. 2023

JAFH

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This study aimed to determine the antibacterial activity of bitter leaf extract (A. paniculata) against the growth of E. tarda bacteria. This test was carried out using Kirby Bauer method (disc test) using five different concentrations, namely 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, and 250 mg/L in TSA (Tryptone Soya Agar) and incubated for 2x24 hours using a temperature of 30 oC. The positive control used was chloramphenicol (5 mg/L), while the negative control used PBS. Bitter leaf extract contains active compounds that have bacteriostatic antibacterial properties. After incubation for 24 hours the concentration of 250 mg/L showed the highest inhibition zone with a diameter of 7.82 mm and decreased at 48 hours to 7.46 mm. After being incubated for 24 hours, the concentration of 250 mg/L obtained an effectiveness value of 57.08% which is the best effectiveness, and there was a decrease in the 48th hour to 54.45%.

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“…The paper discs were then placed on the surface of the media containing inoculated bacteria. The Petri dishes were incubated at 37°C for 18 hours 15,16 .…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial and Antioxidant Activity of Black Mulberry (Morus nigra L.) Extract for Acne Treatment

Budiman¹,

Aulifa²,

Kusuma³

et al. 2017

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Introduction:Black mulberry is rich in phenols and is hence usable in the treatment of acne. It also contains anthocyanin, a well-known antioxidant. This study aimed to examine the antibacterial and antioxidant properties of black mulberry (Morus nigra L.) extract as a potential raw material for use in beauty care products. Method: Fruit extract was obtained using maceration method with 96% ethanol. The antibacterial activity of the extract was determined by disc diffusion method, while the minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by microdilution method. Furthermore, the antioxidant activity of the extract was tested by DPPH method. Result: The results showed a 2.5% MIC against S. epidermidis and P. acnes, confirming the antibacterial activity of black mulberry extract. The MBC values for the respective bacterium were 2.5% and 5%. Regarding antioxidant activity, the IC 50 value of black mulberry extract was 146.731 mg/mL, suggesting its medium potential. Conclusion: Morus nigra extract has antibacterial activity against S. epidermidis and P. acnes, and has medium potential as antioxidant.

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AKTIVITAS ANTIBAKTERI EKSTRAK BUAH ADAS (Foeniculum vulgare, Mill) PADA Vibrio harveyi DAN Vibrio alginolyticus Antibacterial Activity of Fennel (Foeniculum vulgare Mill) Extract on Vibrio alginolyticus and Vibrio harveyi

Cited by 4 publications

References 9 publications

The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro

The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro

Effect of Bitter Leaf Extract (Andrographis paniculata) against Edwardsiella tarda Bacteria In vitro

Antibacterial and Antioxidant Activity of Black Mulberry (Morus nigra L.) Extract for Acne Treatment

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