Aktivitas Antibakteri dari Mikroalga Laut Porphyridium cruentum terhadap Bakteri Staphylococcus epidermidis dan Propionibacterium acne

Anggraeni, Vina Juliana; Nugraha, Fajar Arip; Suhardiman, Aris

doi:10.31764/agrotek.v6i2.1217

Cited by 3 publications

(3 citation statements)

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“…Kurva pertumbuhan bakteri dijadikan sebagai acuan dalam mengidentifikasi fase-fase pertumbuhan bakteri, serta menentukan titik optimum pertumbuhan bakteri. Hal ini bertujuan untuk memaksimalkan produksi probiotik (Anggraeni et al 2019). Hasil penelitian ini menunjukkan titik optimum pertumbuhan BAL asal larva BSF adalah pada jam ke-16 (Gambar 1).…”

Section: Hasil Dan Pembahasanunclassified

Daya Simpan Probiotik Bakteri Asam Laktat asal Larva Black Soldier Fly Terenkapsulasi

Fassah,

Hairani,

Meryandini

et al. 2024

JINTP

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Lactic acid bacteria as probiotic candidates can be isolated from black soldier fly larvae. This study aimed to develop encapsulated lactic acid bacteria (LAB) probiotics from BSF larvae and to evaluate the effect of encapsulation on probiotic viability during 12 weeks of storage at room temperature and 4°C. The experimental design was a factorial completely randomized design with two treatments: storage temperature and storagetime. The variables observed are the number of cells and the level of cell resistance. The result showed that based on the growth curve of LAB isolates from BSF larvae, they could be harvested at 16 hours of age to be produced as probiotics. Encapsulated LAB probiotics from BSF larvae had a population of lactic acid bacteri (LAB) 107 CFU g-1 and were able to maintain the cell viability of 99.3%. Temperature and storage timesignificantly affect (p<0.05) the viability of encapsulated LAB. It can be conclude that encapsulation was able to optimally maintain the LAB viability at temperature of 4°C for 4 weeks of storage. Key words: black soldier fly larvae, encapsulation, probiotic, storability, viability

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Section: Hasil Dan Pembahasanunclassified

Daya Simpan Probiotik Bakteri Asam Laktat asal Larva Black Soldier Fly Terenkapsulasi

Fassah,

Hairani,

Meryandini

et al. 2024

JINTP

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“…The population of these bacteria increased in the 3rd sampling, where in this phase, the bacteria experienced an exponential growth phase. The next phase of growth is the stationary phase or the phase where bacteria do not experience significant growth because they have experienced peak growth in the exponential phase, and the next phase is the death phase [3] Fig. 3.…”

Section: Fig 2 the Growth Of Pathogenic Vibrio Bacteria In The In Viv...mentioning

confidence: 99%

Application of whole cell microalgae as an alternative to Vibriosis prevention

Susianingsih,

Kadriah,

Nurhidayah

2023

E3S Web Conf.

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Increasingly intensive aquaculture activities make the development of the disease increase. One of them is Vibriosis, a disease caused by pathogenic Vibrio bacteria. Several alternative disease prevention have been widely used. One of them is by using microalgae. This study aims to determine the effect of the use of microalgae in whole cells on the growth of pathogenic bacteria. Using 5 types of microalgae, namely Melosira sp, Phaeodactylum sp, Nannochloropsis sp, Porphyridium aerugineum, and Porphyridium sp with 105 cells/ mL of density. In vitro, the challenge test was carried out using a Complete Randomized Design with 6 treatments (5 types of microalgae and 1 control treatment without microalgae), which were repeated 3 times. While in vivo, it was carried out using PL 16 tiger shrimp with a density of 20 heads/jar volume of 20 L. The results showed that in vitro can give whole cell microalgae types P.aerugineum, Nannochloropsis sp, and Melosira sp can prevent the growth of luminous pathogenic Vibrio bacteria. While in vivo administration of microalgae Phaeodactylum sp and Porphyridium aerugineum may inhibit the growth of fluorescent pathogenic Vibrio bacteria better than other microalgae and control.

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“…The higher the concentration, the greater the antibacterial activity, indicated by the size of the diameter of the inhibition zone formed. The mechanism of action of fatty acids as antibacterial, namely causing lysis of bacterial cells by disrupting the permeability of bacterial cell membranes to cause cytoplasm from the cells, this damage results in inhibition of growth to death in bacterial cells [45].…”

Section: Ligandmentioning

confidence: 99%

In Silico Antibacterial Activity of Selected Active Compounds of Cherry Leaf (Muntingia calabura) to Staphylococcus aureus

et al. 2023

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In addition to physical problems, acne caused by Staphylococcus aureus frequently leads to psychological problems as well. As a result, Treatment therapy using antibiotics in the long term and at inappropriate doses increases the rate of antibiotic resistance. Alternative treatments can be performed by using natural ingredients, such as cherry leaves (Muntingia calabura), which exhibit antibacterial properties. This study aimed to determine the active compound of M. calabura extract which has the potential as an antibacterial agent for S. aureus. The research was conducted in September 2022 at Universitas Negeri Surabaya. Using PyRx software, we carried out a molecular docking (blind docking) analysis. The drug-likeness test indicated stigmasterol, tetradecanoic acid, and dodecanoic acid, ethyl ester compounds met the criteria as medicinal compounds. The results of the molecular docking analysis showed that stigmasterol has a binding affinity of -7.8 kcal/mol better than other compounds and RMSD of 1.482 Å indicating its high potential. The three selected active compounds have the potential as antibacterial agents for S. aureus. The application of this product as a treatment for acne, however, requires further research.

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Aktivitas Antibakteri dari Mikroalga Laut Porphyridium cruentum terhadap Bakteri Staphylococcus epidermidis dan Propionibacterium acne

Cited by 3 publications

References 0 publications

Daya Simpan Probiotik Bakteri Asam Laktat asal Larva Black Soldier Fly Terenkapsulasi

Daya Simpan Probiotik Bakteri Asam Laktat asal Larva Black Soldier Fly Terenkapsulasi

Application of whole cell microalgae as an alternative to Vibriosis prevention

In Silico Antibacterial Activity of Selected Active Compounds of Cherry Leaf (Muntingia calabura) to Staphylococcus aureus

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