Akt drives buffalo casein-derived novel peptide-mediated osteoblast differentiation

Reddi, Srinu; Kumar, Naveen; Vij, Rishika; Mada, Sanusi Bello; Kapila, Suman; Kapila, Rajeev

doi:10.1016/j.jnutbio.2016.08.003

Cited by 37 publications

(20 citation statements)

References 59 publications

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“…phorylated AKT increased (P < 0.01) only with Arg (Table 3). The kinase AKT plays an important role in the regulation of MTOR activity by sensing signals related to cell differentiation, proliferation, and apoptosis (Reddi et al, 2016). Despite the change in AKT abundance, protein abundance of MTOR or phosphorylated MTOR did not differ in response to Met or Arg supplementation (Table 3).…”

Section: Genementioning

confidence: 98%

Enhanced supply of methionine or arginine alters mechanistic target of rapamycin signaling proteins, messenger RNA, and microRNA abundance in heat-stressed bovine mammary epithelial cells in vitro

Salama

Duque

Wang

et al. 2019

Journal of Dairy Science

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protein HSPA1A, the apoptotic gene BAX, and the translation inhibitor EIF4EBP1, the mRNA abundance of PPARG, FASN, ACACA (lipogenesis), and BCL2L1 (antiapoptotic) decreased. Greater supply of Met or Arg reversed most of the effects of HS occurring at the mRNA level and upregulated the abundance of HSPA1A. In addition, compared with the control, supply of Met or Arg upregulated genes related to transcription and translation (MAPK1, MTOR, SREBF1, RPS6KB1, JAK2), insulin signaling (AKT2, IRS1), AA transport (SLC1A5, SLC7A1), and cell proliferation (MKI67). Upregulation of microRNA related to cell growth arrest and apoptosis (miR-34a, miR-92a, miR-99, and miR-184) and oxidative stress (miR-141 and miR-200a) coupled with downregulation of fat synthesis-related microRNA (miR-27ab and miR-221) were detected with HS. Results suggest that HS has a direct negative effect on synthesis of protein and fat, mediated in part by coordinated changes in mRNA, microRNA, and protein abundance of key networks. The positive responses with Met and Arg raise the possibility that supplementation with these AA during HS might have a positive effect on mammary metabolism.

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Section: Genementioning

confidence: 98%

Enhanced supply of methionine or arginine alters mechanistic target of rapamycin signaling proteins, messenger RNA, and microRNA abundance in heat-stressed bovine mammary epithelial cells in vitro

Salama

Duque

Wang

et al. 2019

Journal of Dairy Science

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“…Moreover, long‐term treatment with IPP increased mineralization due to enhanced cell survival and matrix formation in human MSCs (Huttunen, Pekkinen, Ahlstrom, & Lamberg‐Allardt, ). Recently, four bioactive peptides with antioxidant and osteoblast proliferation activity were identified from buffalo casein hydrolysates by pepsin–trypsin hydrolysis (Reddi et al, ). Among them, NAVPITPTL markedly increased osteoblast differentiation via activation of pAkt signaling pathway (Reddi, Shanmugam, Kapila, & Kapila, ).…”

Section: Food‐derived Bioactive Peptides: Production and Bone Health mentioning

confidence: 99%

Bone health-promoting bioactive peptides

Ahn

2018

J Food Biochem

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Bioactive peptides, derivatives of proteins, show versatile biological effects and represent potential health-promoting agents as functional food ingredients and/or nutraceuticals. Bone health depends on the balance between bone formation and resorption. When the balance is disrupted, bone diseases such as osteoporosis and fragility fractures may result. Accumulating evidence suggests that peptides derived from endogenous proteins and food proteins enhance bone health.This article reviews the literature on peptides exhibiting bone health-promoting effects. Possible biochemical mechanisms and production of these peptides are briefly discussed.

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“…ALP is highly expressed at early differentiation of the osteoblasts, and thus served as an early differentiation marker [ 33 ]. Moreover, osteocalcin exhibits an important role at later differentiation of the osteoblasts and served as a late differentiation marker [ 34 ]. ALP can hydrolyze pyrophosphate and provide inorganic phosphate for the osteoblasts to promote its mineralization [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…Osteocalcin is the most abundant non-collagenous protein in bone matrix, while carboxylated osteocalcin can bind with hydroxyapatite, resulting in osteocalcin deposition in the extracellular bone matrix [ 36 ]. Collagen peptides can increase both ALP activity and osteocalcin level in MC3T3-E1 [ 22 ], or enhance ALP activity of rat calvarial cells to 227.57 U/mg protein [ 34 ]. Blue mussel ( Mytilus edulis ) protein hydrolysates are verified as being able to stimulate osteoblast differentiation via enhancing ALP activity and osteocalcin production [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

The Growth Proliferation, Apoptotic Prevention, and Differentiation Induction of the Gelatin Hydrolysates from Three Sources to Human Fetal Osteoblasts (hFOB 1.19 Cells)

Ming

Zhao

2018

Molecules

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Gelatins from the skin of bovine, porcine, and tilapia were hydrolyzed to three degrees of hydrolysis (DH) by alcalase, neutrase, and papain, respectively. These hydrolysates at 0.02–0.1 g/L promoted the growth of human fetal osteoblasts by 101.4–135.7%, while higher DH or using papain and tilapia gelatins resulted in higher proliferation. The hydrolysates from porcine and tilapia gelatins at 0.05 g/L prevented induced apoptosis (decreasing total apoptotic proportions from 28.4% or 35.2% to 10.3–17.5% or 16.0–23.6%), and had differentiation induction (increasing alkaline phosphatase activity by 126.9–246.7% in early differentiation stage, or enhancing osteocalcin production by 4.1–22.5% in later differentiation stage). These hydrolysates had a similar amino acid profile; however, tilapia gelatin hydrolysates by papain with DH 15.4% mostly displayed higher activity than others. Tilapia gelatin hydrolysate could up-regulate β-catenin, Wnt 3a, Wnt 10b, cyclin D1, and c-Myc expression at mRNA levels by 1.11–3.60 folds, but down-regulate GSK 3β expression by 0.98 fold. Of note, β-catenin in total cellular and nuclear protein was up-regulated by 1.14–1.16 folds but unchanged in cytoplasmic protein, Wnt 10b, cyclin D1, and c-Myc expression were up-regulated by 1.27–1.95 folds, whilst GSK 3β expression was down-regulated by 0.87 fold. Activation of Wnt/β-catenin pathway is suggested to mediate cell proliferation and differentiation.

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Akt drives buffalo casein-derived novel peptide-mediated osteoblast differentiation

Cited by 37 publications

References 59 publications

Enhanced supply of methionine or arginine alters mechanistic target of rapamycin signaling proteins, messenger RNA, and microRNA abundance in heat-stressed bovine mammary epithelial cells in vitro

Enhanced supply of methionine or arginine alters mechanistic target of rapamycin signaling proteins, messenger RNA, and microRNA abundance in heat-stressed bovine mammary epithelial cells in vitro

Bone health-promoting bioactive peptides

The Growth Proliferation, Apoptotic Prevention, and Differentiation Induction of the Gelatin Hydrolysates from Three Sources to Human Fetal Osteoblasts (hFOB 1.19 Cells)

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