Airborne particulate matter impairs corneal epithelial cells migration via disturbing FAK/RhoA signaling pathway and cytoskeleton organization

Cui, Yu-Hong; Hu, Zixuan; Gao, Zi-Xun; Song, Xi-Ling; Feng, Qing-Yang; Yang, Guang; Li, Zhijie; Pan, Hongwei

doi:10.1080/17435390.2018.1440651

Cited by 47 publications

(33 citation statements)

References 50 publications

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“…Interestingly, not all of our results were consistent with previous findings by Cui et al 18 and Tan et al 19 in support of PM2.5-induced dry eye status. The clinical AC scoring results may share some common characteristics with dry eye symptoms, such as eyelid edema and tearing as early signs of dry eye.…”

Section: Discussionsupporting

confidence: 62%

“…16,17 The main findings were detrimental effects to the corneal epithelium [12][13][14] through increased production of proinflammatory cytokines and changes in tear osmolarity. 16 The only animal studies addressing PM2.5-induced effects on the cornea that have been reported so far lead to evidence of PM2.5 interference in cornea epithelium wound healing 18 and induction of dry eye syndrome. 19 Hence, all previous reports regarding PM2.5 effects on the ocular surface implicated only dry eye conditions, without referring to AC pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…16 Previous animal studies addressing PM2.5-induced cornea damage are relatively few. One study reported by Cui et al 18 demonstrated that PM2.5 may delay cornea epithelium wound healing. Another report by Tan et al 19 showed that PM2.5 exposure led to dry eye syndrome.…”

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confidence: 99%

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A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

Tang

Chang

Chiang

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Several pieces of epidemiologic evidence have indicated PM2.5 (particulate matter with a diameter of 2.5 lm or less) as a causing factor of allergic conjunctivitis, but without experimental elucidation of mechanism. In the present study, PM2.5 in solution was directly applied to the mouse ocular surface to elucidate whether PM2.5 might cause allergic conjunctivitis, and its underlying mechanisms were analyzed. METHODS. ICR mice were challenged for 18 consecutive days with eye drops containing PM2.5 at 3.2, 6.4, and 12.8 mg/mL in 0.9% NaCl saline, along with the controls prepared in parallel without PM2.5 and another control group treated with both PM2.5 at 12.8 mg/mL and artificial tears. On day 19, the whole eyes and meibomian glands were harvested for histopathological analyses and assessment of clinical scoring, tear volume, tear breakup time, and tear ferning. Furthermore, goblet cells by periodic acid Schiff stain and infiltrated eosinophils by Giemsa stain were quantified and compared among study groups. RESULTS. Clinical scoring showed more eyelid edema, tearing, and scratching behaviors, with longer tear breakup time under the influence of increased PM2.5 concentrations. Tear ferning assay showed less tear crystal formation and decreased crystal branches after exposure to PM2.5. In addition, higher goblet cell density in the upper palpebral conjunctiva and extensive eosinophil infiltration in the entire conjunctiva and in the meibomian glands were induced by PM2.5. CONCLUSIONS. These results demonstrate that PM2.5 can induce symptoms similar to clinical allergic conjunctivitis and that the murine acute allergic conjunctivitis model can be induced by direct exposure to PM2.5.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

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A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

Tang

Chang

Chiang

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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“…PM 10 means a PM with a diameter of less than 10 μm, which is about one-fifth of that of human hair. PM stays in the atmosphere and is absorbed into the body through the skin, eyes, and the part of the respiratory tract exposed to the outside [14,15]. PM can act as an irritant or allergen at the primary contact site and causes local adverse effects.…”

Section: Discussionmentioning

confidence: 99%

Relationship between Particulate Matter (PM₁₀) and Airway Inflammation Measured with Exhaled Nitric Oxide Test in Seoul, Korea

Choi

Sim

et al. 2020

Canadian Respiratory Journal

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Purpose. Particulate matter (PM) is increasing every year in Asia. It is not fully understood how the airway is affected when inhaling PM. We investigated the correlation between particulate matter with a diameter of less than 10 μm (PM10) and fractional exhaled nitric oxide (FeNO) to determine whether PM causes airway inflammation. Material and Methods. We analyzed patients who visited our outpatient clinic and tested FeNO from January 2016 to December 2017 at the Korea University Guro Hospital. PM10 data were provided by the government of the Republic of South Korea, and measuring station of PM10 is located 800 meters from the hospital. We analyzed the correlation between PM10 and FeNO by a Pearson correlation analysis and by a multivariate linear regression analysis. To identify the most correlated times, we analyzed the correlation between the FeNO and PM10 daily average from the day of visit to 4 days before visit. Results. FeNO positively correlated with PM10 at two days before hospital visit in the Pearson correlation (Pearson correlation coefficient = 0.057; P-value = 0.023) and in the multivariate linear regression analysis (B = 0.051, P-value = 0.026). If the PM10 increased by 100 μg/m3, the FeNO result was expected to rise to 8.3 ppb in healthy people without respiratory disease. Conclusion. The positive correlation was found in both healthy people and asthmatic patients. Therefore, PM10 can increase airway inflammation.

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“…DES can be caused by various factors such as aging, smoking, health condition, and medications, but it can also be caused by PM exposure. Continuous PM exposure reduces tear film stability, influences the tear osmolality [9], delays corneal epithelium wound healing by inhibiting cell migration [15], and increases oxidation stress on the ocular surface [16]. Furthermore, PAH mixtures increase eye irritation and photosensitivity [17], and high concentrations of heavy metals are associated with DES induction [17,18].…”

Section: Introductionmentioning

confidence: 99%

Topical Application of Liriope platyphylla Extract Attenuates Dry Eye Syndrome Induced by Particulate Matter

Song

Hyun

Lee

et al. 2019

Journal of Ophthalmology

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Particulate matter (PM) is a type of air pollutant that poses a risk to human health. In the ocular system, PM causes or aggravates dry eye syndrome (DES) by damaging the corneal and conjunctival epithelia. Liriope platyphylla has been used traditionally as an expectorant, antitussive agent, and tonic in Korea. However, the effects of Liriope platyphylla extract (LPE) on PM-induced ocular damage have not been elucidated. In this study, we evaluated the in vivo protective effect of LPE against PM-induced DES in rats. Topical administration of LPE attenuated the PM-induced decrease in tear volume and reduced corneal epithelial irregularity and damage. LPE also protected against PM-induced disruption of the corneal mucin-4 layer and reduction in the conjunctival goblet cell density. These findings suggest that LPE has protective effects against PM-induced DES.

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Airborne particulate matter impairs corneal epithelial cells migration via disturbing FAK/RhoA signaling pathway and cytoskeleton organization

Abstract: PM2.5 exposure could result in delay of corneal epithelium wound healing by inhibiting cell migration, thus more attention should be paid to the potential risk of corneal infection and effort should be made to protect eyes against impairment induced by PM2.5.

Cited by 47 publications

References 50 publications

A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

Relationship between Particulate Matter (PM₁₀) and Airway Inflammation Measured with Exhaled Nitric Oxide Test in Seoul, Korea

Topical Application of Liriope platyphylla Extract Attenuates Dry Eye Syndrome Induced by Particulate Matter

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Airborne particulate matter impairs corneal epithelial cells migration via disturbing FAK/RhoA signaling pathway and cytoskeleton organization

Abstract: PM2.5 exposure could result in delay of corneal epithelium wound healing by inhibiting cell migration, thus more attention should be paid to the potential risk of corneal infection and effort should be made to protect eyes against impairment induced by PM2.5.

Cited by 47 publications

References 50 publications

A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

Relationship between Particulate Matter (PM10) and Airway Inflammation Measured with Exhaled Nitric Oxide Test in Seoul, Korea

Topical Application of Liriope platyphylla Extract Attenuates Dry Eye Syndrome Induced by Particulate Matter

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Product

Resources

About

Relationship between Particulate Matter (PM₁₀) and Airway Inflammation Measured with Exhaled Nitric Oxide Test in Seoul, Korea