AIE-based superwettable microchips for evaporation and aggregation induced fluorescence enhancement biosensing

Chen, Yanxia; Min, Xuehong; Zhang, Xiqi; Zhang, Feilong; Lu, Simeng; Xu, Li-Ping; Lou, Xiaoding; Xia, Fan; Zhang, Xueji; Wang, Shutao

doi:10.1016/j.bios.2018.04.011

Cited by 75 publications

(28 citation statements)

References 63 publications

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“…Xu et al reported a superwettable microchip based on AIEgen for biosensing and demonstrated its feasibility for quantifying miRNA (Chen et al, 2018). In their approach, high sensitivity was achieved by taking advantages of the synergetic effect of evaporation-induced enrichment and AIE.…”

Section: Microrna Detectionmentioning

confidence: 99%

Fluorescent Materials With Aggregation-Induced Emission Characteristics for Array-Based Sensing Assay

Zhao

Lai

et al. 2020

Front. Chem.

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Array-based sensing is a powerful tool for identifying analytes in complex environments with unknown interferences. In array-based sensing, the sensors, which transduce binding details to signal outputs, are of crucial importance for identifying analytes. Aggregation-induced emission luminogens (AIEgens) enjoy the advantages of easy synthesis and high sensitivity, which enable them to facilely form a sensor pool through structural modifications and sensitively reflect the subtle changes associated with binding events. All these features make AIEgens excellent candidates for array-based sensing, and attempts have been made by several research groups to explore their potentials in array-based sensing. In this review, we introduce the recent progresses of employing AIEgens as sensors in sensing assays and in building up sensor arrays for identification of varied biological analytes, including biomolecules and bacteria. Examples are selected to illustrate the working mechanism, probe design and selection, capability of the sensor array, and implications of these sensing methods.

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Section: Microrna Detectionmentioning

confidence: 99%

Fluorescent Materials With Aggregation-Induced Emission Characteristics for Array-Based Sensing Assay

Zhao

Lai

et al. 2020

Front. Chem.

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“…Wang et al synthesized an eoteric AIE-based superwettable microchip by associating the superwettable microchips that concentrate by evaporation and the AIEgens together into one chip for sensing microRNA-141 [33] (miR-141) ( Figure 6). By taking advantage of the cooperative effect of the aforementioned two systems, the AIEgens( TPE-Z) were concentrated through evaporation and assembled in as uperhydrophilic microwell and thus achieved stronger fluorescence.…”

Section: Constant Dna Lengthmentioning

confidence: 99%

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

Wang

Huang

et al. 2019

Chemistry An Asian Journal

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DNA occupies significant roles in life processes, which include encoding the sequences of proteins and accurately transferring genetic information from generation to generation. Recent discoveries have demonstrated that a variety of biological functions are correlated with DNA′s conformational transitions. The non‐B form has attained great attention among the diverse forms of DNA over the past several years. The main reason for this is that a large number of studies have shown that the non‐B form of DNA is associated with gross deletions, inversions, duplications, translocations as well as simple repeating sequences, which therefore causes human diseases. Consequently, the conformational transition of DNA between the B‐form and the non‐B form is important for biology. Conventional fluorescence probes based on the conformational transitions of DNA usually need a fluorophore and a quencher group, which suffers from the complex design of the structure and tedious synthetic procedures. Moreover, conventional fluorescence probes are subject to the aggregation‐caused quenching (ACQ) effect, which limits their application toward imaging and analyte detection. Fluorogens exhibiting aggregation‐induced emission (AIE) have attracted tremendous attention over the past decade. By taking advantage of this unique behavior, plenty of fluorescent switch‐on probes without the incorporation of fluorescent quenchers/fluorophore pairs have been widely developed as biosensors for imaging a variety of analytes. Herein, the recent progress in bioanalytical applications on the basis of aggregation‐induced emission luminogens (AIEgens)/nucleic acid nanostructures are presented and discussed.

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“… 3 A few reports have demonstrated detection of DNA, RNA, protein, and bacteria inside stationary droplets by exploiting high sensitivity advantage of evaporation-induced enrichment effect. 3 − 8 Here, to obtain a practical platform for droplet-based assays, we present the use of superhydrophobic organically modified silica-coated surfaces with circular superhydrophilic patterns generated by converting surface-bound methyl groups into hydroxyl groups via UV/ozone treatment. Two-dimensional chemical patterning without physical decomposition resulted in isolation of aqueous droplets in small wetted spots ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Evaporation-Induced Biomolecule Detection on Versatile Superhydrophilic Patterned Surfaces: Glucose and DNA Assay

et al. 2018

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We introduce a droplet-based biomolecular detection platform using robust, versatile, and low-cost superhydrophilic patterned superhydrophobic surfaces. Benefitting from confinement and evaporation-induced shrinkage of droplets on wetted patterns, we show enrichment-based biomolecular detection using very low sample volumes. First, we developed a glucose assay using fluorescent polydopamine (PDA) based on enhancement of PDA emission by hydrogen peroxide (H2O2) produced in enzyme-mediated glucose oxidation reaction. Incubation in evaporating droplets resulted in brighter fluorescence compared to that in bulk solutions. Droplet assay was highly sensitive toward increasing glucose concentration while that in milliliter-volume solutions resulted in no fluorescence enhancement at similar time scales. This is due to droplet evaporation that increased the reaction rate by causing enrichment of PDA and glucose/glucose oxidase as well as increased concentration of H2O2 generated in shrinking droplet. Second, we chemically functionalized wetted patterns with single-stranded DNA and developed fluorescence-based DNA detection to demonstrate the adaptability of the patterned surfaces for a different class of assay. We achieved detection of glucose and DNA with concentration down to 130 μM and 200 fM, respectively. Patterned superhydrophobic surfaces with their simple production, sensitive response, and versatility present potential for bioanalysis from low sample volumes.

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AIE-based superwettable microchips for evaporation and aggregation induced fluorescence enhancement biosensing

Cited by 75 publications

References 63 publications

Fluorescent Materials With Aggregation-Induced Emission Characteristics for Array-Based Sensing Assay

Fluorescent Materials With Aggregation-Induced Emission Characteristics for Array-Based Sensing Assay

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

Evaporation-Induced Biomolecule Detection on Versatile Superhydrophilic Patterned Surfaces: Glucose and DNA Assay

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