Agroecological impact of an in vitro biotechnology approach of embryo development and seed filling in legumes

Abstract

Ongoing global climatic changes and growing demographic pressure have increased demand for agronomic resources and affected the agroecosystem by provoking a number of abiotic stresses that, added to biotic ones, result in physiological and metabolic disorders. Such stresses ultimately impact yield when it most needs to be improved, and understanding and resolving them is a major scientific and agronomic challenge of this century. However, many species are difficult to breed for stress resistance and improved yield for a number of reasons, ranging from a long life cycle (woody species), a reduced genetic background (most self-fertile, cleistogamous legumes) or conversely extensive heterozygosity resulting from an outbreeding nature, and also due to the mainly multigenic origin of such resistances. Biotechnologybased breeding would be an efficient alternative but, for recalcitrant crops, many attempts at in vitro regeneration met with varying degrees of success and often limited to a few genotypes, hampering exploitation of biotechnology approaches. To reduce the risk of undirected somaclonal variations amongst regenerants and transformants, it is better to produce them through somatic embryogenesis that recognises a single-cell origin but whose feasibility is also limited amongst species. There is also a need to fix the resulting genome once a novelty is obtained to ensure efficient heritability of improved traits acquired, which takes several generations in conventional breeding. Acceleration of generations through flowering and fruit set in vitro has been developed in various species including legumes. Haplo-diploidisation in vitro also offers a unique alternative to conventional methods, as it yields novel genetic combinations following doubling of haplotypes and regeneration of fertile plants having gained homozygosity within a single generation. This review will examine the relationships between embryogenesis, stress and its impact on in vitro development of novel genotypes more apt for a sustainable agriculture.

Drought or/and Heat-Stress Effects on Seed Filling in Food Crops: Impacts on Functional Biochemistry, Seed Yields, and Nutritional Quality

“…Moisture content is a key factor controlling seed development, seed filling and metabolic activity of developing seed. The processes related to synthesis and accumulation of various seed reserves are largely influenced by the moisture status of the storage cells, depletion of water at this stage disrupts the seed filling ( Ochatt, 2015 ). The duration of seed filling is inversely proportional to moisture loss and biomass deposition ( Gambín et al, 2007 ).…”

PEG Induces High Expression of the Cell Cycle Checkpoint Gene WEE1 in Embryogenic Callus of Medicago truncatula: Potential Link between Cell Cycle Checkpoint Regulation and Osmotic Stress

“…This situation is exacerbated in arid and semiarid ecosystems. Here, legumes play a central agroecological role through their ability to use atmospheric nitrogen via the symbiosis with Rhizobia, and thus it reduces the need for fertilizers, improve food security, and generally favor the environment ( Rubio et al, 2002 ; Naya et al, 2007 ; Kohler et al, 2008 ; Araújo et al, 2015 ; Ochatt, 2015 ). Studying a legume model species is thus timely and Medicago truncatula is of particular interest given its rather short life cycle and autogamy.…”

Initiation of microcalli in culture of pea (Pisum sativum L.) isolated microspores

“…There are many physical factors affecting the acquisition of competence for in vitro regeneration from cultured cells (Ochatt, 2015). But a reduced number of plants, confirmed as haploids by flow cytometry, were obtained from microspores after pyramiding of multiple (cold, electroporation, osmotic) stress treatments (Ochatt et al, 2009).…”