2016
DOI: 10.1007/978-3-319-45105-3_20
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Agrobacterium tumefaciens-Mediated Transformation of Setaria viridis

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Cited by 22 publications
(24 citation statements)
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“…The time from infection of callus to recovery of mature T1 seed was approximately 4 months. Depending on the composition of the selectable marker gene cassette in the vectors used for transformation, the transformation efficiency, based on infection of the total number of mature seed-derived calli per experiment, ranged from 5 to 15% (Van Eck et al, 2017). Transformation efficiency is defined as the percentage of infected material that gives rise to at least one independent transgenic line.…”
Section: Agrobacterium Tumefaciens- Mediated Transformation Of Maturementioning
confidence: 99%
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“…The time from infection of callus to recovery of mature T1 seed was approximately 4 months. Depending on the composition of the selectable marker gene cassette in the vectors used for transformation, the transformation efficiency, based on infection of the total number of mature seed-derived calli per experiment, ranged from 5 to 15% (Van Eck et al, 2017). Transformation efficiency is defined as the percentage of infected material that gives rise to at least one independent transgenic line.…”
Section: Agrobacterium Tumefaciens- Mediated Transformation Of Maturementioning
confidence: 99%
“…The promoter driving hpt was also found to be key for efficient transformation. Vectors that contained hpt driven by a monocot promoter resulted in recovery of a greater number of transgenic lines compared to vectors where hpt expression was under the control of promoters best suited for dicots (Van Eck et al, 2017). In addition, those vectors that had an intron in the hpt gene resulted in a greater number of transgenic lines than when an intron was not present in the gene.…”
Section: Agrobacterium Tumefaciens- Mediated Transformation Of Maturementioning
confidence: 99%
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