Agrobacterium tumefaciens-Mediated Transformation of Fonsecaea monophora and Fonsecaea erecta for Host-Environment Interaction Studies

Villena, Cristina Isabel Ferrer; Gomes, Renata Rodrigues; Fernandes, Larissa; Florencio, Camille Silva; Bombassaro, Amanda; Grisolia, Maria Eduarda; Trindade, Edvaldo da Silva; Hoog, Sybren de; Vicente, Vânia Aparecida

doi:10.3390/jof6040325

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…Understanding the mechanisms of its pathogenesis will facilitate disease management. ATMT is an effective method for exploring pathogenicity associated genes in pathogenic fungi ( Fitzgerald et al, 2003 ; Leclerque et al, 2004 ; White and Chen, 2006 ; Zhang et al, 2013 ; Li et al, 2019 ; Villena et al, 2020 ; Binh et al, 2021 ; Casado-Del Castillo et al, 2021 ; Chauhan et al, 2021 ; Liu et al, 2021 ). Howerever, this method has not been applied to C. magnum .…”

Section: Discussionmentioning

confidence: 99%

Identifying pathogenicity-related genes in the pathogen Colletotrichum magnum causing watermelon anthracnose disease via T-DNA insertion mutagenesis

Guo

Peng

et al. 2023

Front. Microbiol.

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Fruit rot caused by Colletotrichum magnum is a crucial watermelon disease threatening the production and quality. To understand the pathogenic mechanism of C. magnum, we optimized the Agrobacterium tumefaciens-mediated transformation system (ATMT) for genetic transformation of C. magnum. The transformation efficiency of ATMT was an average of around 245 transformants per 100 million conidia. Southern blot analysis indicated that approximately 75% of the mutants contained a single copy of T-DNA. Pathogenicity test revealed that three mutants completely lost pathogenicity. The T-DNA integration sites (TISs) of three mutants were Identified. In mutant Cm699, the TISs were found in the intron region of the gene, which encoded a protein containing AP-2 complex subunit σ, and simultaneous gene deletions were observed. Two deleted genes encoded the transcription initiation protein SPT3 and a hypothetical protein, respectively. In mutant Cm854, the TISs were found in the 5′-flanking regions of a gene that was similar to the MYO5 encoding Myosin I of Pyricularia oryzae (78%). In mutant Cm1078, the T-DNA was integrated into the exon regions of two adjacent genes. One was 5′-3′ exoribonuclease 1 encoding gene while the other encoded a WD-repeat protein retinoblastoma binding protein 4, the homolog of the MSl1 of Saccharomyces cerevisiae.

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Section: Discussionmentioning

confidence: 99%

Identifying pathogenicity-related genes in the pathogen Colletotrichum magnum causing watermelon anthracnose disease via T-DNA insertion mutagenesis

Guo

Peng

et al. 2023

Front. Microbiol.

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“…Agrobacterium-mediated transformation results in good target gene expression, stable heredity, and low operational costs, and is the main application method in plant transgenic technology research [8][9][10]. However, some researchers still find that the differentiation and regeneration ability of resistant callus obtained by Agrobacterium infection would be reduced even if immature maize embryos are used as starting materials.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize

Liu¹,

Zhou²,

Liu³

et al. 2022

Phyton

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Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, with H8183 having the lowest induction rate (80.1%). The best coculture method was that using the N6 coculture medium layered with a sterile filter paper. Using orthogonal analysis, we found that the optimal combination of the three factors in the differentiation medium was A 3 (1 mg mL −1 IBA), B 3 C 1 (1.6 mg mL −1 6-BA), and D 3 (1.5 mg mL −1 Vc). Through GUS staining analysis, Bar test-strip analysis, and polymerase chain reaction, five transgenic plants were finally obtained. This study established the optimal conditions for genetic transformation in maize.

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Deciphering the roles of bacterial and fungal communities in the formation and quality of agarwood

Fu,

Huang,

Wang

et al. 2024

Stress Biology

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Aquilaria sinensis is a significant resin-producing plant worldwide that is crucial for agarwood production. Agarwood has different qualities depending on the method with which it is formed, and the microbial community structures that are present during these methods are also diverse. Furthermore, the microbial communities of plants play crucial roles in determining their health and productivity. While previous studies have investigated the impact of microorganisms on agarwood formation, they lack comprehensiveness, particularly regarding the properties of the microbial community throughout the entire process from seedling to adult to incense formation. We collected roots, stems, leaves, flowers, fruits and other tissues from seedlings, healthy plants and agarwood-producing plants to address this gap and assess the dominant bacterial species in the microbial community structures of A. sinensis at different growth stages and their impacts on growth and agarwood formation. The bacteria and fungi in these tissues were classified and counted from different perspectives. The samples were sequenced using the Illumina sequencing platform, and sequence analyses and species annotations were performed using a range of bioinformatics tools to assess the plant community compositions. An additional comparison of the samples was conducted using diversity analyses to assess their differences. This research revealed that Listeria, Kurtzmanomyces, Ascotaiwania, Acinetobacter, Sphingobium, Fonsecaea, Acrocalymma, Allorhizobium, Bacillus, Pseudomonas, Peethambara, and Debaryomyces are potentially associated with the formation of agarwood. Overall, the data provided in this article help us understand the important roles played by bacteria and fungi in the growth and agarwood formation process of A. sinensis, will support the theoretical basis for the large-scale cultivation of A. sinensis, and provide a basis for further research on microbial community applications in agarwood production and beyond.

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Agrobacterium tumefaciens-Mediated Transformation of Fonsecaea monophora and Fonsecaea erecta for Host-Environment Interaction Studies

Cited by 3 publications

References 43 publications

Identifying pathogenicity-related genes in the pathogen Colletotrichum magnum causing watermelon anthracnose disease via T-DNA insertion mutagenesis

Identifying pathogenicity-related genes in the pathogen Colletotrichum magnum causing watermelon anthracnose disease via T-DNA insertion mutagenesis

Optimization of Agrobacterium tumefaciens-Mediated Genetic Transformation of Maize

Deciphering the roles of bacterial and fungal communities in the formation and quality of agarwood

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