2015
DOI: 10.1016/j.jgeb.2015.09.001
|View full text |Cite
|
Sign up to set email alerts
|

Agrobacterium tumefaciens – Mediated transformation of Woodfordia fruticosa (L.) Kurz

Abstract: In the present study, a protocol for Agrobacterium tumefaciens-mediated transformation has been optimized for Woodfordia fruticosa (L.) Kurz. Precultured axenic leaf segments were cocultivated with A. tumefaciens strain LBA4404 harboring the binary plasmid pCAMBIA1301 with b-glucuronidase (uidA) containing intron as the reporter gene and hygromycin phosphotransferase (hpt) as a selectable marker gene. After 3 days of co-cultivation, leaf segments were cultured on MS medium containing Thidiazuron (TDZ 4.54 lM) … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2018
2018
2023
2023

Publication Types

Select...
3

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(2 citation statements)
references
References 20 publications
(20 reference statements)
0
2
0
Order By: Relevance
“…The insertion of foreign genes into the genome of plants mediated by Agrobacterium tumefaciens is an effective and reproducible method and has been successfully applied to various plants such as Artemisia carvifolia [5], Woodfordia fruticosa [6], Solanum trilobatum [7], Withania somnifera [8], Vanda kasem's [9], and Erycina pusilla [10].…”
Section: Introductionmentioning
confidence: 99%
“…The insertion of foreign genes into the genome of plants mediated by Agrobacterium tumefaciens is an effective and reproducible method and has been successfully applied to various plants such as Artemisia carvifolia [5], Woodfordia fruticosa [6], Solanum trilobatum [7], Withania somnifera [8], Vanda kasem's [9], and Erycina pusilla [10].…”
Section: Introductionmentioning
confidence: 99%
“…For the genetic transformation of plants, the preculture of leaf explants can promote cell division, which helps Agrobacterium infection and aid the integration of exogenous DNA, thus enhancing transformation efficiency (Birch 1997, Bullea et al, 2015. This was likely due to the likeliness of T-strand integration into cells that actively underwent cell cycle progression (Villemont et al, 1997).…”
Section: Discussionmentioning
confidence: 99%