Agrobacterium spp. genes and ORFs: Mechanisms and applications in plant science

Rangslang, Rishan Kupar; Liu, Ziqi; Lütken, Henrik; Favero, Bruno Trevenzoli

doi:10.1590/1413-70542018425000118

Cited by 11 publications

(9 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the rolB geneencoded product was shown to exhibit tyrosine phosphatase activity (Filippini 1996;Dilshad et al 2015) and to interact with 14-3-3 proteins (Moriuchi et al 2004;Matveeva et al 2015), thus taking part in auxin signaling, enhancing the sensitivity of transformed cells toward auxin. It has been suggested that the product of rolC gene is a glucosidase liberating cytokinins from their bound forms (Estruch et al 1991;Rangslang et al 2018). Besides, the individual or combined rol genes are recognized to be an e cient activator of secondary metabolites biosynthesis in plants (Sarkar et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Elicitor-induced phenolic acids accumulation in Salvia virgata Jacq. hairy root cultures

Dowom

Abrishamchi

Radjabian

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Phenolic acids, as the predominant secondary metabolites of Salvia species, are largely used in pharmaceutical industries. The main aim of the study was to establish hairy root cultures of Salvia virgata Jacq. Also, the effects of methyl jasmonate (22.4 and 11.2 ppm), Ag + ions (5 and 2.5 ppm), and yeast extract (100 and 50 ppm) were assessed on total phenol, total avonoid, rosmarinic acid, salvianolic acid A, and caffeic acid contents in the hairy roots after 1, 3 and 5 days of exposure. Results showed the used Agrobacterium rhizogenes strains (A4, ATCC15834, R1000, GM1534, and C58C1) differed in their ability to induce hairy roots on leaf explants. The transformed roots were molecularly con rmed using rolC gene and the highest transformation frequency (56 %) was obtained by ATCC 15834 strain. Among the established hairy root lines, the highest amount of rosmarinic acid (0.45 ± 0.01 mg/g DW) and dry root biomass (2.29 ± 0.04 g) was obtained in AT3, the line which was induced by ATCC 15834 strain. The maximum accumulation of total phenol (123.6 ± 0.93 mg GAE/g DW), total avonoid (5.09 ± 0.07 mg QUE/g DW), rosmarinic acid (18.45 ± 0.8 mg/g DW), salvianolic acid A (2.11 ± 0.04 mg/g DW) and caffeic acid (2.61 ± 0.02 mg/g DW) was observed in the hairy roots elicited with 22.4 ppm methyl jasmonate on day three after treatment. The results support that elicitation could be an effective procedure for the improvement of caffeic acid derivatives production in S. virgata hairy root cultures.Furthermore, it has shown that RA and Sal-B could improve memory and cognitive impairment related to Alzheimer's disease (Shen et al. 2017;Gok et al. 2018). Chemically, CA is the basic structural unit of phenolic acids in Salvia species. Rosmarinic acid is an ester of CA and 3, 4-dihydroxyphenyllactic acid, and Sal-B is identi ed as a dimmer of RA (Wang et al. 2019) (Fig. 1).Biosynthesis of phenolic acids occurs mainly via the phenylpropanoid and tyrosine-derived pathways (Di et al. 2013). Many of the encoding genes for the critical enzymes in the biosynthetic pathways of phenolic acids, including phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), hydroxycinnamate coenzyme A ligase (4CL), tyrosine aminotransferase (TAT), 4-hydroxyphenylpyruvate reductase (HPPR), rosmarinic acid synthase (RAS), and a cytochrome P450-dependent monooxygenase (CYP98A14), were identi ed in S. miltiorrhiza (Di et al. 2013;Zhang et al. 2014;Wang et al. 2015;Xing et al. 2018b).Due to the genetic stability, large biomass output, and high biosynthetic capacity, hairy root (HR) culture is considered as an alternative technique for secondary metabolite production, compared to native plant roots and cell/callus cultures (Hu and Du 2006;Ono and Tian 2011). Moreover, the application of HR cultures in many industrial and scienti c elds, including designing of bioreactors, recombinant protein production, phytoremediation, and metabolic and genetic engineering, has been reported (Ashihara et al. 2011;Doran 2013; Kuma 2018).It has demonstrated that the ...

show abstract

Section: Discussionmentioning

confidence: 99%

Elicitor-induced phenolic acids accumulation in Salvia virgata Jacq. hairy root cultures

Dowom

Abrishamchi

Radjabian

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…La bacteria, naturalmente incorpora genes que producen tumores o son inductores de la producción de hormonas vegetales en el huésped. Los biotecnólogos han reemplazado esos sitios y en su lugar insertan genes con algún interés de expresión (Rangslang et al, 2018). El sistema de A. tumefaciens y la entrega del plásmido Ti en la célula huésped se basa en el acompañamiento de varias proteínas que "escoltan" el ADN a través de la membrana del huésped y hasta el núcleo del mismo; a estas proteínas se les conocen como Vir, hay de varios tipos, cada una con una función específica.…”

Section: Flores Aromáticas: ¿Es Posible?unclassified

Bases para la mejora genética en la rosa del desierto (Adenium obesum)

Orozco

Gonzalez²

2021

View full text Add to dashboard Cite

Adenium obesum es una planta de la familia Apocynaceae con alto valor ornamental. El atractivo y diversidad de sus flores junto a la porción de tallo engrosado que presenta, le confieren un elevado atractivo. A pesar de ser una planta muy apreciada en la jardinería y altamente comercializada, existen muy pocos estudios sobre genética. El mercado de plantas ornamentales exige la creación constante de nuevas variedades de A. obesum para aumentar la oferta, sin embargo, el mejoramiento genético que se ha realizado en la especie ha sido convencional mediante cruces y no se han realizado técnicas biotecnológicas. Objetivo: hacer una consolidación de información acerca de la genética de A. obesum para crear la base de un futuro programa de mejora genética. Se tuvo en cuenta aspectos como: características generales de la especie, morfología floral, métodos de propagación, polinización manual, autoincompatibilidad y diferentes técnicas que pueden apoyar un programa de mejora.

show abstract

“…Numerous genes and open reading frames (ORFs) are present on the T L -DNA. The most well-known and characterized genes known to be involved in the compact habit and reduced plant growth, are the root oncogenic loci (rol) genes; rolA, rolB, rolC, and rolD, which correspond to ORFs 10, 11, 12, and 15, respectively (White et al, 1985;Slightom et al, 1986; reviewed by Rangslang et al, 2018;De Paolis et al, 2019). In comparison, the T R -DNA harbors aux1 and aux2 genes (iaaM and iaaH, respectively; Camilleri and Jouanin, 1991) involved in auxin biosynthesis (Mashiguchi et al, 2019) as well as a homolog of the rolB gene, termed rolBTr (Bouchez and Camilleri, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Plants can be regenerated from the hairy roots following treatment with various, often high, and ratios of cytokinins:auxins (Hegelund et al, 2017;Mandal et al, 2020;Neumann et al, 2020). Regenerated plants exhibit, among other features, compact habit, reduced plant growth, and frequently thicker leaves that are often reduced in size (as reviewed by Lütken et al, 2012a;Rangslang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Transgenic Kalanchoë blossfeldiana, Containing Individual rol Genes and Open Reading Frames Under 35S Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers

et al. 2021

Self Cite

View full text Add to dashboard Cite

Reduced growth habit is a desirable trait for ornamental potted plants and can successfully be obtained through Rhizobium rhizogenes transformation in a stable and heritable manner. Additionally, it can also be obtained by transformation with Agrobacterium tumefaciens harboring specific genes from R. rhizogenes. The bacterial T-DNA harbors four root oncogenic loci (rol) genes and 14 less known open reading frames (ORFs). The four rol genes, i.e., rolA, rolB, rolC, and rolD, are conceived as the common denominator for the compact phenotype and the other less characterized ORFs seem auxiliary but present a potential breeding target for less aberrant and/or more tailored phenotypes. In this study, Kalanchoë blossfeldiana ‘Molly’ was transformed with individual rol genes and selected ORFs in 35S overexpressing cassettes to comprehensively characterize growth traits, gene copy and expression, and ethylene tolerance of the flowers. An association of reduced growth habit, e.g. height and diameter, was observed for rolB2 and ORF14-2 when a transgene single copy and high gene expression were detected. Chlorophyll content was reduced in overexpressing lines compared to wild type (WT), except for one ΔORF13a (a truncated ORF13a, where SPXX DNA-binding motif is absent). The flower number severely decreased in the overexpressing lines compared to WT. The anthesis timing showed that WT opened the first flower at 68.9 ± 0.9 days and the overexpressing lines showed similar or up to 24 days delay in flowering. In general, a single or low relative gene copy insertion was correlated to higher gene expression, ca. 3 to 5-fold, in rolB and ΔORF13a lines, while in ORF14 such relation was not directly linked. The increased gene expression observed in rolB2 and ΔORF13a-2 contributed to reducing plant growth and a more compact habit. Tolerance of detached flowers to 0.5 μl L−1 ethylene was markedly higher for ORF14 with 66% less flower closure at day 3 compared to WT. The subcellular localization of rolC and ΔORF13a was investigated by transient expression in Nicotiana benthamiana and confocal images showed that rolC and ΔORF13a are soluble and localize in the cytoplasm being able to enter the nucleus.

show abstract

Agrobacterium spp. genes and ORFs: Mechanisms and applications in plant science

Cited by 11 publications

References 102 publications

Elicitor-induced phenolic acids accumulation in Salvia virgata Jacq. hairy root cultures

Elicitor-induced phenolic acids accumulation in Salvia virgata Jacq. hairy root cultures

Bases para la mejora genética en la rosa del desierto (Adenium obesum)

Transgenic Kalanchoë blossfeldiana, Containing Individual rol Genes and Open Reading Frames Under 35S Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers

Contact Info

Product

Resources

About