Agrobacterium-mediated transformation of the cultivated strawberry (Fragaria × anannassa duch.) using disarmed binary vectors

James, D. J.; Passey, A. J.; Barbara, D. J.

doi:10.1016/0168-9452(90)90106-x

Cited by 84 publications

(37 citation statements)

References 28 publications

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“…6d). These optima of inoculation time and density have also been effective in a number of transformation reports for F. ananassa (James et al 1990;Nehra et al 1990; Barceló et al 1998).…”

Section: Factors Influencing Agrobacterium-mediated Transformationmentioning

confidence: 90%

Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens

et al. 2002

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By identifying antibiotics that eliminated Agrobacterium tumefaciens with the least phytotoxic effects, we were able to select appropriate A. tumefaciens strains for a more efficient transformation of seasonal Fragaria vesca and everbearing F. v. semperflorens. An efficient and reproducible method of shoot regeneration from leaf discs was developed with optimal shoot regeneration obtained on medium supplemented with 0.25 mg l -1 indole-3-butyric acid and 3 mg l -1 benzyladenine for both F. vesca forms. Petiole sections were also effective for F. v. semperflorens regeneration, and culture vessel capacity was found to influence shooting from both explant types and F. vesca forms. Of a range of antibiotics commonly used for Agrobacterium elimination, carbenicillin had the least inhibitory effect on shoot regeneration and was found to effectively control strain LBA4404. Both F. vesca forms showed a high sensitivity to kanamycin and, therefore, a selection regime ramped from 10 mg l -1 to 25 mg l -1 kanamycin over a period of 8 weeks was developed. Optimal transformation efficiency (15%) was achieved by the appropriate use of explant type and age, leaf-disc orientation, Agrobacterium density and inoculation time and phenolic compounds for bacterial virulence induction. Southern analysis confirmed the integration of the β-glucuronidase reporter gene into the genomic DNA of both F. vesca L. forms.

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“…6d). These optima of inoculation time and density have also been effective in a number of transformation reports for F. ananassa (James et al 1990;Nehra et al 1990; Barceló et al 1998).…”

Section: Factors Influencing Agrobacterium-mediated Transformationmentioning

confidence: 90%

Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens

et al. 2002

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“…Although our transgenic apple clones were initially produced at 60 mg/1 we are currently using 25 mg/1 since we know that this permits no root formation in control untransformed Green sleeves material that normally gives very high rooting levels [ 11]. The use of the different selection strategies and their effects on the regeneration of apple transformants have been discussed elsewhere [ 11,16]. Although we have obtained transgenic apple plants with selection periods as short as 5 days this only slightly reduces the time taken for transgenic shoots to regenerate compared to shoots regenerated after much longer periods of exposure to the antibiotic.…”

Section: ) the Selection Of Transformed Cells That Lead To The Regenmentioning

confidence: 99%

“…of media per well can be added (see Fig. 1 in [ 16 ]). These dishes have the advantage of separating explants from each other so that should contamination occur during the lengthy incubation time required for regeneration and transformation not all explants will be lost, as is often the case when using non-compartmented Petri dishes.…”

Section: Culture Vessels For Micropropagation and Regenerationmentioning

confidence: 99%

Regeneration and Transformation of Apple (Malus pumila Mill.)

James

Dandekar

1991

Plant Tissue Culture Manual

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“…The possibility of that cell metabolism converts cefotaxime to a compound with growth regulator activity has been discussed [16]. Low concentration of cefotaxime enhanced shoot regeneration in wheat [16,17] maize [18], apple [19] and strawberry [20]. Similarly regeneration efficiency was reduced by increasing the concentration of cefotaxime in three plants such as maize, apple and wheat and inhibits in common snapdragon [21] and tobacco [22].…”

Section: Effect Of Cefotaxime On In Vitro Plantmentioning

confidence: 99%

The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on <i>In Vitro</i> Plant Regeneration of <i>Centella asiatica</i> (L.)—An Important Antijaundice Medicinal Plant

Panathula¹,

Mahadev²,

Naidu³

2014

AJPS

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Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 µM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 µM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 µM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0 mg/L). This combination generates 90% of regeneration frequency and maximum number of roots per shoot (14.2) and high root length (4.2 cm). The rooted plants were acclimatized and transferred to field for survivalance. The addition of antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.

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Agrobacterium-mediated transformation of the cultivated strawberry (Fragaria × anannassa duch.) using disarmed binary vectors

Cited by 84 publications

References 28 publications

Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens

Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens

Regeneration and Transformation of Apple (Malus pumila Mill.)

The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on <i>In Vitro</i> Plant Regeneration of <i>Centella asiatica</i> (L.)—An Important Antijaundice Medicinal Plant

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Agrobacterium-mediated transformation of the cultivated strawberry (Fragaria × anannassa duch.) using disarmed binary vectors

Cited by 84 publications

References 28 publications

Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens

Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens

Regeneration and Transformation of Apple (Malus pumila Mill.)

The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on &lt;i&gt;In Vitro&lt;/i&gt; Plant Regeneration of &lt;i&gt;Centella asiatica&lt;/i&gt; (L.)—An Important Antijaundice Medicinal Plant

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The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on <i>In Vitro</i> Plant Regeneration of <i>Centella asiatica</i> (L.)—An Important Antijaundice Medicinal Plant