Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes coding bacterial antigens from Mycobacterium tuberculosis

Матвєєва, Н. А.; Vasylenko, M. Yu.; Shakhovsky, A. M.; Кучук, М. В.

doi:10.3103/s0095452709020042

Cited by 14 publications

(7 citation statements)

References 19 publications

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“…Genetic fusions allowed the expression of fusion proteins carrying two or more components with the aim to elicit immune responses against different targets, including antigens from distinct pathogens or strains. To increase immunogenicity, the ESAT6 antigen was fused with other tuberculosis antigens (Ag85B and Mtb72F) [ 3 , 20 , 40 , 41 ] or adjuvants (CTB: cholera toxin B subunit, LTB: Escherichia coli heat-labile enterotoxin B subunit, LipY: a cell wall protein, and ELP: elastin-like peptide) [ 3 , 7 , 42 ] and expressed in various plant species ( Arabidopsis thaliana , tobacco, and lettuce).…”

Section: Discussionmentioning

confidence: 99%

Transgenic Carrot Expressing Fusion Protein ComprisingM. tuberculosisAntigens Induces Immune Response in Mice

Permyakova

Zagorskaya

Belavin

et al. 2015

BioMed Research International

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Tuberculosis remains one of the major infectious diseases, which continues to pose a major global health problem. Transgenic plants may serve as bioreactors to produce heterologous proteins including antibodies, antigens, and hormones. In the present study, a genetic construct has been designed that comprises the Mycobacterium tuberculosis genes cfp10, esat6 and dIFN gene, which encode deltaferon, a recombinant analog of the human γ-interferon designed for expression in plant tissues. This construct was transferred to the carrot (Daucus carota L.) genome by Agrobacterium-mediated transformation. This study demonstrates that the fusion protein CFP10-ESAT6-dIFN is synthesized in the transgenic carrot storage roots. The protein is able to induce both humoral and cell-mediated immune responses in laboratory animals (mice) when administered either orally or by injection. It should be emphasized that M. tuberculosis antigens contained in the fusion protein have no cytotoxic effect on peripheral blood mononuclear cells.

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Section: Discussionmentioning

confidence: 99%

Transgenic Carrot Expressing Fusion Protein ComprisingM. tuberculosisAntigens Induces Immune Response in Mice

Permyakova

Zagorskaya

Belavin

et al. 2015

BioMed Research International

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“…Biological synthesis of CdS nanoparticles was performed using hairy root culture of L. maroccana L. Hairy root culture was obtained using Agrobacterium -mediated transformation as described by us early [ 18 ]. Plant culture was grown in a liquid hormone free MS medium [ 19 ] at 28°C during 10 days.…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis of luminescent CdS quantum dots using plant hairy root culture

et al. 2014

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CdS nanoparticles have a great potential for application in chemical research, bioscience and medicine. The aim of this study was to develop an efficient and environmentally-friendly method of plant-based biosynthesis of CdS quantum dots using hairy root culture of Linaria maroccana L. By incubating Linaria root extract with inorganic cadmium sulfate and sodium sulfide we synthesized stable luminescent CdS nanocrystals with absorption peaks for UV-visible spectrometry at 362 nm, 398 nm and 464 nm, and luminescent peaks at 425, 462, 500 nm. Transmission electron microscopy of produced quantum dots revealed their spherical shape with a size predominantly from 5 to 7 nm. Electron diffraction pattern confirmed the wurtzite crystalline structure of synthesized cadmium sulfide quantum dots. These results describe the first successful attempt of quantum dots synthesis using plant extract.PACS81.07.Ta; 81.16.-c; 81.16.Rf

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“…The studies on antituberculosis vaccines involving plant expression systems and transferring the sequence of M. tuberculosis gene esat6 to plants are rather few [ 16 – 21 ]. Fusion of the ESAT6 antigen with other tuberculosis antigens, such as Ag85B or Mtb72F [ 17 , 18 , 20 , 22 ], and use of various adjuvants, such as CTB, LTB, LipY, or ELP [ 17 , 19 , 21 ] with further expression in various plant species ( Arabidopsis thaliana, tobacco, and lettuce), have been attempted. For this purpose, various plant expression systems have been utilized, namely, agroinfiltration [ 16 , 18 ] as well as nuclear [ 17 , 19 , 20 , 22 ] and chloroplast [ 21 ] transformations.…”

Section: Introductionmentioning

confidence: 99%

Oral Immunogenicity of Plant-MadeMycobacterium tuberculosisESAT6 and CFP10

Уварова

Belavin

Permyakova

et al. 2013

BioMed Research International

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Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data suggest that the proteins in question are appropriate as a candidate edible vaccine against tuberculosis.

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Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes coding bacterial antigens from Mycobacterium tuberculosis

Cited by 14 publications

References 19 publications

Transgenic Carrot Expressing Fusion Protein ComprisingM. tuberculosisAntigens Induces Immune Response in Mice

Transgenic Carrot Expressing Fusion Protein ComprisingM. tuberculosisAntigens Induces Immune Response in Mice

Biosynthesis of luminescent CdS quantum dots using plant hairy root culture

Oral Immunogenicity of Plant-MadeMycobacterium tuberculosisESAT6 and CFP10

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