Transgenic Carrot Expressing Fusion Protein Comprising<i>M. tuberculosis</i>Antigens Induces Immune Response in Mice

Permyakova, N. V.; Zagorskaya, A. A.; Belavin, P. A.; Уварова, Е. А.; Nosareva, Olesya V.; Nesterov, Andrey E.; Novikovskaya, Anna; Zavyalov, Evgeniy L.; Мошкин, М. П.; Дейнеко, Е. В.

doi:10.1155/2015/417565

Cited by 25 publications

(22 citation statements)

References 43 publications

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“…Till date, no study has been reported regarding expression of any vaccine antigen in broccoli. The expression of ESAT‐6 protein obtained in the present study is fairly high compared with previously expressed ESAT‐6 antigen in edible plants using nuclear transformation.…”

Section: Discussionmentioning

confidence: 55%

“…Results show that the orally delivered recombinant ESAT‐6 expressed in broccoli plant and purified ESAT‐6 derived from transgenic broccoli have the potential to induce humoral response. Previous studies have also shown that ESAT‐6 from M. tuberculosis had the potential to elicit the cellular and humoral responses in mice . Results of oral immunization using the raw plant biomass are of special interest because it is the most advantageous immunization strategy in terms of cost and easy application.…”

Section: Discussionmentioning

confidence: 99%

“…It has been proven that stimulation of cell‐mediated immune mechanisms, including γδ T cells, CD4+ T cells, CD8+ T cells, and CD1‐restricted and HLA‐E‐restricted T cells, have the potential to kill the mycobacterium . Some of the most promising antigens of MTB that could be used for subunit vaccine development are: ESAT‐6, MTB72F, Ag85B, and LiPY . 6 kDa early secretory antigenic target (ESAT‐6) is one of the most important subunit vaccine antigen of MTB, which is encoded in the region of deletion (RD‐1) in all strains of MTB except in the BCG vaccine strains .…”

Section: Introductionmentioning

confidence: 99%

“…These emerging bioencapsulated vaccine antigens expressed in plants provide a cost‐effective platform to deliver different therapeutic proteins in living organisms. For TB, oral‐based mucosal vaccination is most suitable for initiation of mucosal and systemic immune response because MTB is a respiratory infectious agent .…”

Section: Introductionmentioning

confidence: 99%

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Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant

Saba

Sameeullah

Asghar

et al. 2020

Biotech and App Biochem

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Tuberculosis (TB) is one of the major infectious diseases caused by Mycobacterium tuberculosis. The development of an effective and economical vaccine for controlling TB is essential especially for developing countries. Edible plants can serve as biofactories to produce vaccine antigens. In this study, 6 kDa early secretory antigenic target (ESAT‐6) of M. tuberculosis was expressed in Brassica oleracea var. italica via Agrobacterium‐mediated transformation to facilitate oral delivery of antigen. ESAT‐6 gene was cloned using Gateway® cloning strategy. Transformation and presence of transgene was confirmed through PCR. Expression level of transgene was calculated via quantitative real‐time PCR (qRT‐PCR) and the maximum integrated transgene number was two. Maximum amount of total soluble fraction of ESAT‐6 was evaluated by immunoblotting, estimated to accumulate up to 0.5% of total soluble protein. The recombinant ESAT‐6 protein was further purified and detected using silver staining and Western blotting. ESAT‐6 protein induced humoral immune response in mice immunized orally and subcutaneously. The expression of M. tuberculosis antigen in edible plants could aid in the development of cost‐effective and oral delivery of an antigen‐based subunit vaccine against TB. To the best our knowledge, it is the first report of expression of a vaccine antigen in broccoli.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant

Saba

Sameeullah

Asghar

et al. 2020

Biotech and App Biochem

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“…Previous studies have demonstrated that corresponding protein antigens may be successfully expressed following DNA vaccine administration, and these protein antigens are capable of simulating a natural infection and producing a more comprehensive immune response (8,9). The antigen-specific cellular and humoral immune responses must be detected following administration of a DNA vaccine directed against a pathogenic microorganism or malignant tumor, in order to evaluate the immune effects of the DNA vaccine.…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of varicella zoster virus glycoprotein E DNA vaccine

Wei

Gan

Ren

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. In the present study a eukaryotic expression vector of varicella zoster virus (VZV) glycoprotein E (gE) was constructed and enabled to express in COS7 cells. Furthermore, a specific immune response against the VZV gE eukaryotic expression plasmid was induced in BALB/c mice. The VZV gE gene was amplified using polymerase chain reaction (PCR) and cloned into a eukaryotic expression vector, pcDNA3.1. The recombinant vector was subsequently transfected into COS7 cells using a liposome transfection reagent. The recombinant protein was instantaneously expressed by the transfected cells, as detected by immunohistochemistry, and the recombinant pcDNA-VZV gE plasmid was subsequently used to immunize mice. Tissue expression levels were analyzed by reverse transcription-PCR. In addition, the levels of serum antibodies and spleen lymphocyte proliferation activity were investigated. The amplified target gene included the full-length gE gene (~2.7 kb), and the recombinant expression vector induced gE expression in COS7 cells. In addition, the expression plasmid induced sustained expression in vivo following immunization of mice. Furthermore, the plasmid was capable of inducing specific antibody production and effectively stimulating T cell proliferation. Effective humoral and cellular immunity was triggered in the mice immunized with the VZV gE eukaryotic expression vector. The results of the present study laid the foundation for future research into a VZV DNA vaccine.

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