Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: A non-tissue culture approach

Feldmann, Kenneth A.; Marks, M. David

doi:10.1007/bf00330414

Cited by 450 publications

(221 citation statements)

References 29 publications

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“…Considering the number of mutations that have been isolated, this is a relatively small number of genes on the genetic map. Trisomic and telotrisomic lines have also been identified in Arabidopsis which can be used for linkage studies (Koornneef 1983 A number of different tissues of Arabidopsis including roots, leaves, stems, and seeds are susceptible to A~robacterium-mediated T-DNA transformation, (Lloyd et al 1986;Schmidt and Willmitzer 1988;Valvekens et al 1988;Feldmann and Marks 1987). (Leutwiler et al 1984;Meyerowitz and Pruitt 1985).…”

Section: -------mentioning

confidence: 99%

Mapping genes essential for embryo development in Arabidopsis thaliana

1991

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Section: -------mentioning

confidence: 99%

Mapping genes essential for embryo development in Arabidopsis thaliana

1991

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“…We have developed the in planta transformation protocol for Sunnhemp which is tissue culture independent based on the transformation reports of crops like Arabidopsis thaliana (Feldmann and Mark 1987), legumes like Soya bean and also on the reports that the cotyledonary nodes in Sunnhemp are the major sites of gene transfer (Hiroyuki daimon et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics

Rao¹,

Agrawal²,

Mahmood

et al. 2011

Physiol Mol Biol Plants

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A transformation system which is free of in vitro plant regeneration following Agrobacterium infection is established for the forage legume, Sunnhemp (Crotalaria juncea L.) where in the entire embryo axis of the germinating seed was used as the target tissue for transformation. After standardization of transformation conditions, the cotyledonary node of the embryo axis was infected with Agrobacterium host LBA 4404 harboring the recombinant vector pCAMBIA 2301. The bivalent 1D gene of the two major foot and mouth disease virus (FMDV) serotypes 'O' and 'A 22 ' and the neomycin phosphotransferase (nptII) gene were used as the markers for optimization of the protocol. The embryo axes were pricked randomly on the cotyledonary node and co-cultivated with Agrobacterium. The germlings were then allowed to grow under standard growth room conditions in to mature fertile plants. 60 T 0 plants were established from 3 separate experiments. Three hundred seeds from the 60 T 0 plants were sown to raise the T 1 generation of which 180 were analyzed for integration of bivalent FMDV gene 1D "O" and "A 22 " and the nptII gene. Eighteen out of these 180 plants amplified both the marker genes. Two independent transgenic lines 24 and 37, showed elevated levels of expression of 12 μg and 8 μg (per gm of fresh leaf) of the bivalent ID antigen "O" and "A 22 " . The results showed that the transformation efficiency was 3 %. To the best of our knowledge, this is the first successful attempt of Agrobacterium tumefaciens mediated transformation of Sunnhemp. The protocol can generate whole plant transformants with relative ease and should be compatible to all genotypes of Sunnhemp.

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“…K-134, transgenics were developed by a tissue culture independent Agrobacterium-mediated in planta transformation strategy. The in planta transformation protocols have been developed for transformation in many crop species, Arabidopsis thaliana (Feldmann and Marks 1987), soybean (Trick and Finer 1997), sunflower (Rao and Rohini 1999), safflower (Rohini and Rao 2000), rice (Supartana et al 2005), maize (Chumakov et al 2006) and cotton (Keshamma et al 2008). Earlier an in planta transformation protocol was developed in groundnut by Rohini and Rao (2000) and proved its reproducibility by Keshamma et al (2008).…”

Section: Discussionmentioning

confidence: 99%

Transgenics in groundnut (Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.)

Keshavareddy

Rohini

Ramu

et al. 2013

Physiol Mol Biol Plants

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Large number of primary transgenic events were generated in groundnut by an Agrobacterium mediated, in planta transformation method to assess the efficacy of cry1AcF against the Spodoptera litura. generation demonstrated homozygous nature. This clearly proved that though there is considerable improvement in average mean % larval mortality in T 2 generation, the cry1AcF gene was effective against S. litura only to some extent.

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Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: A non-tissue culture approach

Cited by 450 publications

References 29 publications

Mapping genes essential for embryo development in Arabidopsis thaliana

Mapping genes essential for embryo development in Arabidopsis thaliana

Tissue culture independent transformation of the forage crop sunnhemp (Crotalaria juncea L.): an easy method towards generation of transgenics

Transgenics in groundnut (Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.)

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