Agrobacterium-Mediated Transformation of Cotton (Gossypium Hirsutum L.) Using Dmo Gene for Enhanced Tolerance Against Dicamba Pesticide

Abstract

The agrobacterium based transformation of herbicide-resistant crops has modernized weed management in crops by producing cost-effective and ecosystem friendly transgenic plants. Cotton is one of the major crops which are grown worldwide due to its great economic value in textile industries. Dicamba is a commonly used herbicide in broadleaf plants to kill a wide range of weeds in many dicotyledonous crop fields since the 20th century. In this study, Eagle 2 cotton variety was transformed with the DMO gene which is responsible for the synthesis of the Dicamba monooxygenase enzyme that exhibits tolerance against the Dicamba herbicide. This entire study was conducted at Four Brothers Genetics Lab, Lahore. Transformed cultures of Agrobacterium Tumefaciens with the DMO gene were acquired. Cotton embryos were isolated and co-cultivated with transformed Agrobacterium cultures under sterile conditions. Transformed embryos were grown on an artificial growth medium and acclimatized under favorable conditions. Healthy and stable plants were shifted infield where they were grown into a mature plant. Leaf samples of these plants were collected and DNA was successfully isolated by the CTAB method. Transformed plants were confirmed by Polymerase chain reaction and gel electrophoresis. Variations in different traits among transformed cotton plants were found which indicated that the transgenic plant 4 showed higher plant height, monopodial and sympodial branches, leaf length, leaf width, number of bolls, and bolls weight. The better performance of plant 4 indicated that the yield potential of the transgenic plant was improved as compared with other transgenic cotton plants.

First record of Fusarium brachygibbosum as a causal agent of seed decay and damping-off disease on cotton in Iraq and Control using some bioagents

“…Cotton (Gossypium hirsutum L.) is one of the most important economic fiber crops, which is essential in agriculture and trade and which is grown to obtain fiber, which is used in the textile industry, and for obtaining oil from its seeds: 1 . Cotton has a tremendous economic place in the world in general and Iraq in particular, as it is considered one of the cash crops that generate profits for farmers and its uses in many industries, 2 .…”

Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum

“…Cotton (Gossypium spp. ), is genetically diverse plant, it has four domesticated species such as G. arboreum L, G. herbaceum L, G. barbadense L. and G. hirsutum L. G. arboreum has many remarkable benefits over G. hirsutum, and has significant resistance against biotic and abiotic stresses especially drought and salinity, which makes it valuable gene pool for improving modern cotton cultivars [7][8][9] .Gossypium Universal stress Protein-2 (GUSP-2) has been identified and cloned from water stressed leaves of G. arboreum 10,11 . Sequence analysis showed that GUSP-2 is highly similar to bacterial MJ0577-type of…”

“…Cotton (Gossypium spp. ), is genetically diverse plant, it has four domesticated species such as G. arboreum L, G. herbaceum L, G. barbadense L. and G. hirsutum L. G. arboreum has many remarkable benefits over G. hirsutum, and has significant resistance against biotic and abiotic stresses especially drought and salinity, which makes it valuable gene pool for improving modern cotton cultivars [7][8][9] .…”