Agrobacterium-mediated transformation of Artemisia absinthium L. (wormwood) and production of secondary metabolites

Nin, Stefania; Bennici, A.; Roselli, G.; Mariotti, D.; Schiff, Silvia; Magherini, R.

doi:10.1007/s002990050310

Cited by 44 publications

(15 citation statements)

References 1 publication

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“…Similar affects have been observed in other plant species; e.g. eucalyptus (Machado et al, 1997); wormwood (Nin et al, 1997) and lentil (Hassan et al, 2007).…”

Section: Effect Of a Tumefaciens Strains On Crown-supporting

confidence: 54%

Factors Enhancing Virulence Response of Agrobacteria in Cucumber (<i>Cucumis sativus</i> L.)

Mohiuddin

Abdullah²,

Chowdhury

et al. 2010

Agriculturists

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The virulence response of five wild type Agrobacterium tumefaciens (tumorigenic) strains and five wild type A. rhizogenes (oncogenic) strains was studied in cucumber (Cucumis sativus L.), cv. Spring Swallow. Several physical and chemical factors e.g. Agrobacterium strains, tissue type (stem and petiole tissues), light intensity, dark incubation, concentrations of acetosyringone (a phenolic compound) were tested. The virulent response of Agrobacteria was found to be depend on plant tissue type, Agrobacterium strain, tissue-Agrobacterium interaction and both chemical-physical factors. Specific light intensity, 2500-lux, as well as the acetosyringone concentration, 20-M, played important role in virulence response. Among the different strains, LBA 4404 and 15834 were highly virulent to cucumber compared to the other strains tested. A. tumefaciens strains A348 and A281 were failed to initiate crown galls in both stem and petiole tissues, however, in the presence of acetosyringone they produced crown galls. Similarly, A. rhizogenes strain A4 initiated hairy roots in the presence of acetosyringone. All developed crown galls and hairy roots showed an autonomous growth on a hormone free medium containing cefatoxime. Thus, enhancement of virulence response was not only dependent on Agrobacterium strains, but also on particular physical and chemical factors also play vital role.

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“…Similar affects have been observed in other plant species; e.g. eucalyptus (Machado et al, 1997); wormwood (Nin et al, 1997) and lentil (Hassan et al, 2007).…”

Section: Effect Of a Tumefaciens Strains On Crown-supporting

confidence: 54%

Factors Enhancing Virulence Response of Agrobacteria in Cucumber (<i>Cucumis sativus</i> L.)

Mohiuddin

Abdullah²,

Chowdhury

et al. 2010

Agriculturists

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“…They were cut and transferred to MS medium for more growth. As Nin et al (1997) reported on A. absintium L., they were covered with numerous hairs and highly branched (Fig. 4b).…”

Section: Hairy Roots Inductionmentioning

confidence: 49%

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Sharafi

Sohi

Mirzaee

et al. 2014

Physiol Mol Biol Plants

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In the present study, we developed an efficient protocol for in vitro plant regeneration and genetically transformed root induction in medicinal plant Artemisia aucheri Boiss. Leaf explants were cultivated in MS medium supplemented by combination of plant growth regulators including α-naphthalene-acetic acid, 6-benzyl-aminopurine, indole-3-acetic acid and 2, 4-dichlorophenoxyaceticacid. The highest frequency of shoot organogenesis occurred on MS medium supplemented with 0.05 mg/l NAA plus 2 mg/l BA (96.3 %) and MS medium supplemented with 0.5 mg/l IAA plus 2 mg/l BA (88.3 %). Root induction was obtained on MS medium supplemented with 0.5 mg/l IBA. This is a simple, reliable, rapid and high efficient regeneration system for A. aucheri Boiss in short period via adventitious shoot induction approach. Also, an efficient genetically transformed root induction for A. aucheri was developed through Agrobacterium rhizogenes-mediated transformation by four bacterial strains, A4, ATCC15834, MSU440, and A13 (MAFF-02-10266). The maximum frequency of hairy root induction was obtained using MSU440 (93 %) and ATCC15834 (89 %) bacterial strains. Hairy root lines were confirmed by PCR using the rolB gene specific primers and Southern blot analysis.

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“…The extract causes hallucinations, seizures and the Vincent van Gogh illness, contributing to psychoses and suicides (31). A. absinthium (and A. maritima), a perennial undershrub with aromatic properties, has traditional medical applications as an antihelminthic, insecticidal, stomachic, and tonic-bitter (70). The distribution and consumption of absinthe, however, has been banned in numerous countries because of the toxic properties despite its later distribution under the name Pernod.…”

Section: Artemisia Sppmentioning

confidence: 99%

“…Artemisinin levels were higher in A. tumefaciens-transformed shoot teratomas of A. annua than in control cultures even though amoxicillin or ticarcillin and a β-lactamase inhibitor could effectively eliminate the Agrobacterium (105). A. absinthium hairy root cultures produced novel compounds different from those of conventional root cultures (70).…”

Section: Artemisia Sppmentioning

confidence: 99%

Important Secondary Metabolites and Essential Oils of Species Within the Anthemideae (Asteraceae)

Silva

Yonekura

Kaganda

et al. 2005

Journal of Herbs, Spices & Medicinal Plants

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Agrobacterium-mediated transformation of Artemisia absinthium L. (wormwood) and production of secondary metabolites

Cited by 44 publications

References 1 publication

Factors Enhancing Virulence Response of Agrobacteria in Cucumber (<i>Cucumis sativus</i> L.)

Factors Enhancing Virulence Response of Agrobacteria in Cucumber (<i>Cucumis sativus</i> L.)

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Important Secondary Metabolites and Essential Oils of Species Within the Anthemideae (Asteraceae)

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