Agrobacterium mediated transfer of a mutant Arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (Cichorium intybus L.)

Vermeulen, A.; Vaucheret, Hervé; Pautot, Véronique; Chupeau, Yves

doi:10.1007/bf00235074

Cited by 25 publications

(7 citation statements)

References 16 publications

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“…The combinations of NAA with either KIN or BAP produced similar results while the combination of IAA with KIN was significantly more successful than that with BAP in terms of the mean number of shoots produced per explant (18.3 shoots vs. 10.7 shoots, respectively). This finding is consistent with the results of Vermeulen et al (1992) who demonstrated that IAA was the most potent type of auxin for the regeneration of witloof chicory. Another noteworthy observation was that the combination of 0.3 mg l -1 IAA with 0.5 mg l -1 KIN produced nearly four times more shoots per explant than when 0.5 mg l -1 KIN was used alone (18.3 shoots per explant compared to 5.5 shoots, Table 2).…”

Section: Discussionsupporting

confidence: 95%

TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.)

Yücesan

Türker

Gürel

2007

Plant Cell Tiss Organ Cult

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A very efficient and rapid regeneration system via multiple shoot formation was developed for Cichorium intybus L. when leaf explants excised from sterile seedlings were cultured on medium supplemented with different concentrations and combinations of various plant growth regulators. In a comparison of leaf lamina and petiole explants, lamina explants produced over three times more shoots than petiole explants, with a mean of 7.5 shoots compared to 2.4. Of the combinations of KIN/ IAA, KIN/NAA, BAP/IAA, or BAP/NAA, 0.5 mg l -1 KIN combined with 0.3 mg l -1 IAA was the most effective, producing a mean of 19.7 shoots per lamina explant while the control treatment involving no plant growth regulators produced no shoots at all. When either cytokinin was used alone, BAP was found nearly twice more successful than KIN. However, the most effective treatment of all was the combination of 0.01 mg l -1 TDZ and 1.0 mg l -1 IAA, producing as many as 35.8 shoots per lamina explant. This rate of shoot regeneration is remarkably higher than those previously reported for C. intybus, most likely due to the highly inductive effect of TDZ, which was tested for the first time in this species. Rooting of the shoots was readily achieved on medium containing different concentrations of IAA or IBA. IAA was more effective than IBA and resulted in the highest frequency of shoots that rooted (100%) and mean number of roots per shoot (4.2) when used at 0.5 mg l -1 . Hardening off process resulted in a production of more than 80% healthy plantlets.

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Section: Discussionsupporting

confidence: 95%

TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.)

Yücesan

Türker

Gürel

2007

Plant Cell Tiss Organ Cult

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“…In contrast to the situation for root chicory, appropriate herbicides are available for sugarbeet and chemical weed control in Jerusalem artichoke is not generally necessary. Since resistance to glyphosate and sulfonylurea herbicides has been found in chicory (Sellin et al, 1992; Vermeulen et al, 1992; Lavigne et al, 1994), herbicide‐resistant root chicory cultivars may offer a new herbicide option in the near future.…”

Section: Resultsmentioning

confidence: 99%

Agronomic Performance of Root Chicory, Jerusalem Artichoke, and Sugarbeet in Stress and Nonstress Environments

Schittenhelm¹

1999

Crop Science

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the traditional classification, according to the primary storage carbohydrate, into sucrose-, fructan-, and starch-Introduction of the fructan crops root chicory (Cichorium intybus crops, is becoming increasingly inappropriate. A recent L.; RC) and Jerusalem artichoke (Helianthus tuberosus L.; JA) into example is the creation of a fructan beet through inagricultural production systems is desirable to diversify crop rotation. Field experiments were conducted to compare the agronomic perfor-serting into sugarbeet a single gene from Jerusalem artimance of RC and JA with sugar beet (Beta vulgaris L.; SB). One choke that encodes an enzyme which converts sucrose set of cultivars during 1995, 1996, and 1997 and an additional set in 1997 to fructan (Sé venier et al., 1998). From an agroecological were grown on a sandy loam soil (Haplic Luvisol) at Braunschweig, point of view, it is desirable to have for each kind of Germany. Crops were cultivated with and without supplemental irrigaagricultural raw products one or few alternative crops, tion, complete and no weed control (1995 and 1996), and N fertilization instead of a single multiple-use major crop. However, rates of 0, 60, and 120 kg ha Ϫ1 . Severe water stress caused significant under present conditions in agriculture, root chicory and but similar storage organ yield losses in all crops, whereas mild water Jerusalem artichoke will only become true alternative stress mainly affected JA yields. Averaged across years and N levels, sugar crops if the raw materials are produced at prices storage organ yield losses through weed competition under irrigation competitive to those of established high yielding crops. amounted to 70, 47, and 8% in SB, RC, and JA, respectively. Averaged across years, in the absence of water and weed stress, SB, RC, and

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“…This vector construct was used to transform leaf discs of Nicotiana tabacum var. Samson NN and Cichorium intybus by Agrobacterium tumefaciens strain LB4044 following published procedures [13,14]. Chicory transformation was carried out at the biotechnology department of Nunhems Zaden, Haelen, The Netherlands.…”

Section: Transformation and Plant Materialsmentioning

confidence: 99%

Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose:fructan 6‐fructosyltransferase

Sprenger

Schellenbaum

Dun³

et al. 1997

FEBS Letters

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We have recently cloned a cDNA encoding sucrose:-fructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the ~-2,6 linkages typical of the grass fructans, graminans and phleins Proc. Natl. Acad. Sci. USA 92, 11652-11656). Here we report functional expression of 6-SFT from barley in transgenic tobacco and chicory. Transformants of tobacco, a plant naturally unable to form fructans, synthesized the trisaccharide kestose and a series of unbranched fructans of the phlein type (13-2,6 linkages). Transformants of chicory, a plant naturally producing only unbranched fructans of the inulin type (~-2,1 linkages), synthesized in addition branched fructans of the graminan type, particularly the tetrasaccharide bifurcose which is also a main fructan in barley leaves.

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Agrobacterium mediated transfer of a mutant Arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (Cichorium intybus L.)

Cited by 25 publications

References 16 publications

TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.)

TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.)

Agronomic Performance of Root Chicory, Jerusalem Artichoke, and Sugarbeet in Stress and Nonstress Environments

Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose:fructan 6‐fructosyltransferase

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