Agrobacterium-mediated in planta transformation of maize germ cells

Mamontova, Evgeniya; Velikov, V. А.; Volokhina, I. V.; Chumakov, M. I.

doi:10.1134/s1022795410040186

Cited by 17 publications

(4 citation statements)

References 9 publications

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“…Ever since Feldmann and Marks (1987) established in planta transformation for Arabidopsis, several researchers have successfully used it for various crops by manipulating a number of parameters, including Agrobacterium strains, target tissue, pre-culture duration, bacterial virulence modifiers and treatments that prime target tissues to Agrobacterium infection (Supartana et al 2005;Tague and Mantis 2006;Seol et al 2008;Akbulut et al 2008;De Oliveira et al 2009;Li et al 2009a;Chen et al 2010;Subramanyam et al 2013;Mayavan et al 2013). Agrobacterium-mediated in planta transformation has been successfully established for monocotyledonous plants such as wheat, rice, and maize (Supartana et al 2006;Lin et al 2009;Mamontova et al 2010).…”

Section: Introductionmentioning

confidence: 98%

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

et al. 2015

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An efficient, reproducible, and genotype-independent in planta transformation has been developed for sugarcane using setts as explant. Traditional Agrobacterium-mediated genetic transformation and in vitro regeneration of sugarcane is a complex and time-consuming process. Development of an efficient Agrobacterium-mediated transformation protocol, which can produce a large number of transgenic plants in short duration is advantageous. Hence, in the present investigation, we developed a tissue culture-independent in planta genetic transformation system for sugarcane using setts collected from 6-month-old sugarcane plants. The sugarcane setts (nodal cuttings) were infected with three Agrobacterium tumefaciens strains harbouring pCAMBIA 1301-bar plasmid, and the transformants were selected against BASTA(®). Several parameters influencing the in planta transformation such as A. tumefaciens strains, acetosyringone, sonication and exposure to vacuum pressure, have been evaluated. The putatively transformed sugarcane plants were screened by GUS histochemical assay. Sugarcane setts were pricked and sonicated for 6 min and vacuum infiltered for 2 min at 500 mmHg in A. tumefaciens C58C1 suspension containing 100 µM acetosyringone, 0.1 % Silwett L-77 showed the highest transformation efficiency of 29.6 % (with var. Co 62175). The three-stage selection process completely eliminated the chimeric transgenic sugarcane plants. Among the five sugarcane varieties evaluated using the standardized protocol, var. Co 6907 showed the maximum transformation efficiency (32.6 %). The in planta transformation protocol described here is applicable to transfer the economically important genes into different varieties of sugarcane in relatively short time.

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Section: Introductionmentioning

confidence: 98%

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

et al. 2015

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“…8. Использование в качестве способа переноса агробактериальной Т-ДНК в геном во время оплодотворения (Mamontova et al, 2010).…”

Section: Fig 5 Inhibition Of the R-nj Gene Phenotypic Manifestationunclassified

The prospects for using haploinducers in maize breeding

Асадова

Ульянов

Карлов

et al. 2020

Biotehnologiâ i selekciâ rastenij

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The discovery of spontaneous haploid plants and the development of ways to produce them in in vitro culture have set a new direction important for breeding and for theoretical research in reproductive biology. The frequency of spontaneous haploidy in cultivated plants is extremely low and does not exceed 0.01-0.1%, therefore, the search for sources and donors capable of stimulating haploidy in hybrid combinations is of current interest. Expansion of the search for new sources and donors of the haploinduction trait, the creation of new, more effective haploinducers contribute to the accumulation of scientific information and genetic sources, characterized by a high resource potential for selection and genetic research. The causеs of haploidy are not well understood yet. According to the available information, the genes localized in the qhir1, qhir11, qhir12 regions of chromosome 1 in maize are responsible for this process. The use of genes that stimulate haploinduction in maize in combination with the marker gene R1-nj responsible for anthocyanin coloration of the caryopsis and embryo, as well as genes A1 and B1, which are in control of the entire plant coloration, allowed the creation of haploinducer lines with a frequency of haploid stimulation up to 15%. Phenotypic expression of dominant alleles of the marker anthocyanin coloration genes in different parts of a hybrid plant, as well as in the caryopsis and embryo, contributes to the high-quality selection of haploid kernels in the cob due to the manifestation of recessive alleles of these genes at the haploid level. The presence of anthocyanin synthesis suppressor genes in siliceous maize (C1-I, C2-Idf, In1-D) restricts the use of the R1-nj gene in other representatives of siliceous maize. In order to overcome this problem, studies are underway to create other genotypes of haploinducers, which are not associated with the anthocyanin coloration of the caryopsis, but instead have other marker traits, such as the oil content in the kernel, the absence of ligules in the leaves, and root coloration in seedlings. The use of matroclinous and androclinous types of haploinduction allows breeders to obtain highly homozygous dihaploid maize lines, with both the maternal and paternal genomes. These achievements made it possible to cut five or more times the material and time inputs into the creation of inbred lines and their sterile analogs, accelerate the breeding of new maize hybrids, and signifi cantly improve the quality of seed production in terms of typicality and uniformity. The materials presented in the article should help breeders and geneticists to learn more about the innovative directions and problems of hybrid maize breeding.

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“…Most of them lead to production of chimeras and somaclonal variation. Among the alternate methods for development of transgenic plants (Rakoczy-Trojanowska 2002), methods such as the floral dip method (Clough and Bent 1998), pollen transformation (Wang et al 2001), pistil transformation (Chumakov et al 2006;Mamontova et al 2010) and ovary-dip transformation (Yang et al 2009) have been found to be easier, avoiding the in vitro phase and requisite for regeneration. In planta methods such as floral dip method, although demonstrated in a few species such as Arabidopsis thaliana (Clough and Bent 1998), is yet to be extended to other species.…”

Section: Abstract Pollen Grains Transformation Transgenic Plantsmentioning

confidence: 99%

Pollen grains as a target for introduction of foreign genes into plants: an assessment

Eapen

2011

Physiol Mol Biol Plants

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Agrobacterium-mediated in planta transformation of maize germ cells

Cited by 17 publications

References 9 publications

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

The prospects for using haploinducers in maize breeding

Pollen grains as a target for introduction of foreign genes into plants: an assessment

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