Agrobacterium-Mediated Gene Delivery in Various Tissues and Genotypes of Wheat (Triticum aestivum L.)

Mahalakshmi, Akella; Khurana, Paramjit

doi:10.1007/bf03262953

Cited by 12 publications

(5 citation statements)

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“…Biolistics can also be utilised to deliver DNA into the genomes of mitochondria and chloroplasts. Perceived advantages, particularly in terms of lower copy number have driven significant research into using Agrobacterium tumefaciens (synonym Rhizobium radiobacter) for the DNA transfer process, and many laboratories now report successful wheat transformation using Agrobacterium (Binka et al 2012;Campa et al 2005;Cheng et al1997;Cheng et al 2003;Ding et al 2009;Guo et al 1998;Haliloglu and Baenziger 2003;Hamid et al 2012;He et al 2010;Jones et al 2005;Khanna and Daggard 2003;Mahalakshmi and Khurana 1995;Marks et al 1989;McCormac et al 1998;Mitic et al 2004;Mooney et al 1991;Murin et al 2011;Parrott et al 2002;Patnaik et al 2006;Pérez-Piñeiro et al 2012;Przetakiewicz et al 2004;Pukhalskii et al 1996;Rashid et al 2012;Song et al 2012;Tamas-Nyitrai et al 2012;Trifonova et al 2001;Wu et al 2003Wu et al , 2008Xia et al 1999). One particular unpublished method developed by the Japan Tobacco company (Pure-Wheat Technology) has been licensed to specific laboratories who report wheat transformation efficiencies of more than 50 %.…”

Section: Methods For Wheat Transformationmentioning

confidence: 99%

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

Azhakanandam¹,

Silverstone²,

Daniell³

et al. 2015

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Section: Methods For Wheat Transformationmentioning

confidence: 99%

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

Azhakanandam¹,

Silverstone²,

Daniell³

et al. 2015

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“…Biolistics can also be utilised to deliver DNA into the genomes of mitochondria and chloroplasts. Perceived advantages, particularly in terms of lower copy number have driven significant research into using Agrobacterium tumefaciens (synonym Rhizobium radiobacter) for the DNA transfer process, and many laboratories now report successful wheat transformation using Agrobacterium (Binka et al 2012;Campa et al 2005;Cheng et al1997;Cheng et al 2003;Ding et al 2009;Guo et al 1998;Haliloglu and Baenziger 2003;Hamid et al 2012;He et al 2010;Jones et al 2005;Khanna and Daggard 2003;Mahalakshmi and Khurana 1995;Marks et al 1989;McCormac et al 1998;Mitic et al 2004;Mooney et al 1991;Murin et al 2011;Parrott et al 2002;Patnaik et al 2006;Pérez-Piñeiro et al 2012;Przetakiewicz et al 2004;Pukhalskii et al 1996;Rashid et al 2012;Shi et al 2011;Song et al 2012;TamasNyitrai et al 2012;Trifonova et al 2001;Wu et al 2003Wu et al , 2008Xia et al 1999). One particular unpublished method developed by the Japan Tobacco company (PureWheat Technology) has been licensed to specific laboratories who report wheat transformation efficiencies of more than 50 %.…”

Section: Methods For Wheat Transformationmentioning

confidence: 99%

Wheat Biotechnology: Current Status and Future Prospects

Jones

2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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In 2010, wheat was grown on nearly 217 million ha worldwide (more than any other food crop) and a total of 651 million t of grain was harvested, making it the third most-cultivated cereal after maize and rice (www.faostat.fao.org). It is one of the main staple food crops providing approximately 20 % of our daily needs of protein and calories. Wheat is traded on global commodity markets which have become accustomed to a good annual harvest and large reserves. However, global demand, estimated at 666 million metric tons (MMT) in 2010, is predicted to increase in line with population growth and reach 880 MMT (a 40 % increase) by 2050 (Weigand 2011). Significant action will be required to maintain this positive situation.Further complicating the supply/demand balance are extreme weather events, unpredictable longer-term climate patterns and changing regional preferences in crop choice/suitability. Recent localised severe weather and poor harvests have resulted in price volatility with notable spikes in 2008 and 2012 (Fig. 8.1). The volatility in the wheat market was exacerbated by the Russian export ban in 2010 resulting from an extreme heatwave in the grain producing regions of southwestern Russia. The highest July temperatures in 130 years brought severe drought, causing the annual harvest of the biggest wheat producing region (Volga) to be reduced by more than 70 % on that of the previous year (Wegren 2011; Welton 2011). The US wheat supplies for 2013/2014 are projected at 2917 million bushels, down 7 % from 2012/2013 (Vocke and Liefert 2014) and there is evidence that growers in some US states are moving away from wheat in favour of biotech soya and corn. Quoting statistics from the US Department of Agriculture, Rookhuyzen (2012) stated that wheat acreage for South Dakota declined by 1.2 million acres, or 28 %, between 1981 and 2011 and the acres planted with spring wheat in 2012 were the lowest in

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“…[9] повідомили про стабільну трансформацію свіжих виділених незрілих зародків, прекультивованих незрілих зародків та ембріогенного калюсу та підтвердили успішну передачу екзогенних генів у наступні покоління рослин. Крім того, були випробувані деякі інші експланти, зокрема такі, як проростки 1-4 добового віку [10], апікальні меристеми зародків сухого насіння [11], базальні частини листя [12]. Останнім часом значно зріс інтерес до апікальної меристеми пагонів як найбільш перспективного експланта для злакових культур [13], оскільки його перевагою є можливість подолання генотипічних особливостей форм, що характеризуються низьким регенераційним потенціалом, а також можливість отримання значної кількості вихідного матеріалу за короткий час.…”

Section: результати та обговоренняunclassified

Optimization of Agrobacterium-mediated transformation of perspective winter wheat genotypes in vitro

Дубровна¹,

Slivka²

2020

Fakt. eksp. evol. org.

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Aim. Optimization of conditions for genetic transformation of new perspective winter wheat genotypes. Methods. Agrobacterium-mediated transformation in culture in vitro. Results. The influence of the optical density of cells of the agrobacterial suspension, the concentration of the antibiotic cefotaxime, the duration of coculture on the frequency of obtaining kanamycin-resistant regenerants of new winter wheat genotypes by genetic transformation of callus cultures were investigated using LBA4404 and AGL0 strains. It is shown that depending on the strain the most optimal is the concentration of agrobacteria 0.2-03 OD, duration of coculture for 2-3 days and the use of cefotaxime at a concentration of 250-500 mg/L. Conclusions. The optimal parameters for conducting Agrobacterium-mediated transformation of callus cultures of new perspective winter wheat genotypes were selected. Keywords: Triticum aestivum, Agrobacterium-mediated transformation, callus cultures.

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Agrobacterium-Mediated Gene Delivery in Various Tissues and Genotypes of Wheat (Triticum aestivum L.)

Cited by 12 publications

References 24 publications

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

Wheat Biotechnology: Current Status and Future Prospects

Optimization of Agrobacterium-mediated transformation of perspective winter wheat genotypes in vitro

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