Agrobacterium-mediated Cry1A(b) gene transfer in Punica granatum L. cv. Kandhari Kabuli using different In Vitro regeneration pathways

Verma, Vipasha; Kanwar, Kamlesh; Tufchi, Mahak; Kashyap, M. K.

doi:10.1007/s12892-013-0033-6

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…141,148,150,153,155 Crytoxins are the most commonly used insectresistant proteins that are being used since last few decades for the purposes of integrated pest management with an application to cotton, soybean, and maize. [164][165][166] Research is also underway to identify genes to ensure resistance against insects that remain unaffected by Cry genes. Various genes for the development of disease-resistant plants have been reported.…”

Section: Need For Gmos and Genes For Pakistanmentioning

confidence: 99%

Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops

et al. 2019

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Transgenic technologies have emerged as a powerful tool for crop improvement in terms of yield, quality, and quantity in many countries of the world. However, concerns also exist about the possible risks involved in transgenic crop cultivation. In this review, literature is analyzed to gauge the real intensity of the issues caused by environmental stresses in Pakistan. In addition, the research work on genetically modified organisms (GMOs) development and their performance is analyzed to serve as a guide for the scientists to help them select useful genes for crop transformation in Pakistan. The funding of GMOs research in Pakistan shows that it does not follow the global trend. We also present socioeconomic impact of GM crops and political dimensions in the seed sector and the policies of the government. We envisage that this review provides guidelines for public and private sectors as well as the policy makers in Pakistan and in other countries that face similar environmental threats posed by the changing climate.

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Section: Need For Gmos and Genes For Pakistanmentioning

confidence: 99%

Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops

et al. 2019

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“…Currently, there is few efficient way to reduce the fruit borer attack in pomegranate. Genetic engineering offers a promising way to combat the insect invasion for the effective and available genes from other organisms [10] which has been employed to develop fruit crops with improved horticultural traits such as disease resistance, pest resistance, herbicide tolerance, cold tolerance, salt tolerance, and improved shelf life, etc [11]. The most common approaches are Agrobacterium-mediated transformation and particle bombardment where tissue culture processes are required.…”

Section: Introductionmentioning

confidence: 99%

Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation

Yang¹

2016

PLANT

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Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.

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“…The entire plant is used in traditional medicines for blood pressure, dyspesia, epilepsy, blood purification and liver disorders (Anonymous, 1976;Kirtikar and Basu 1984;de Rus Jucquet et al, 2014;Malla et al, 2015). The herb is very effective against gastrointestinal infection (Mukherji, 1953), used as antipyretic, hypoglycemic (Saxena and Mukherjee, 1992;Bhargava et al, 2009;Verma et al, 2013), antiperiodic, antifungal, (Chakravarty et al, 1994;Rehman et al, 2011), hepatoprotective (Mukherjee et al, 1997), anti-inflammatory (Banerjee et al, 2000), antispasmodic (Saha and Das, 2001), antibacterial (Joshi and Dhawan, 2005), antioxidative (Scartezzini and Speroni, 2000) and used to treat malaria and diabetes (Kumar and Staden, 2016). Recently, Swertia chirayita extract has been reported to possess anti-Hepatitis B virus activity (Zhou et al, 2015).…”

mentioning

confidence: 99%

Phytochemical and Thin Layer Chromatographic Evaluation of Swertia chirayita Buch.-Hams. Ex Wall at Different Developmental Stages

Kumari¹,

Guleria²,

Kaur³

2019

Int.J.Curr.Microbiol.App.Sci

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Swertia chirayita a medicinal herb indigenous to temperate Himalaya, belongs to family Gentianaceae, consist of 180 species (Hooker, 1885). The common name of Swertia chirayita is chiretta. Swertia found from Kashmir to Bhutan at an altitude of 1200-3000 m amsl and in the Khasi hills at 1200-1500 m amsl (Kirtikar and Basu, 1984; Pradhan and Badola, 2010). But, 2000 m altitude is highly preferable range (Bhattarai and Acharya, 1996). It is also found in open ground and recently slash and burnt forests (Edwards, 1993). The genus Swertia Linn. consists of annual and perennial herbs. Some authors have described Swertia chirayita as an annual (Anonymous, 1982; Kirtikar and Basu, 1984) and others as biennial or pluriannual herb (Edwards, 1993). It has about 2-3 ft long and erect stem, having orange brown or purplish in colour and contain large continuous yellowish pith

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Agrobacterium-mediated Cry1A(b) gene transfer in Punica granatum L. cv. Kandhari Kabuli using different In Vitro regeneration pathways

Cited by 6 publications

References 27 publications

Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops

Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops

Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation

Phytochemical and Thin Layer Chromatographic Evaluation of Swertia chirayita Buch.-Hams. Ex Wall at Different Developmental Stages

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Agrobacterium-mediated Cry1A(b) gene transfer in Punica granatum L. cv. Kandhari Kabuli using different In Vitro regeneration pathways

Cited by 6 publications

References 27 publications

Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops

Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops

Observation of GFP Expression in Pomegranate (&lt;i&gt;Punica granatum&lt;/i&gt; L.) Via Pollen-Mediated Transformation

Phytochemical and Thin Layer Chromatographic Evaluation of Swertia chirayita Buch.-Hams. Ex Wall at Different Developmental Stages

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Product

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Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation