Agricultural surface water, imidacloprid, and chlorantraniliprole result in altered gene expression and receptor activation in Pimephales promelas

Stinson, Sarah; Hasenbein, Simone; Connon, Richard E.; Deng, Xin; Alejo, Jordan S.; Lawler, Sharon P.; Holland, Erika B.

doi:10.1016/j.scitotenv.2021.150920

Cited by 17 publications

(25 citation statements)

References 58 publications

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“…This was expected from the concentrations used in the present study (3–300 µg L −1 ), which were mostly below the LC 50 thresholds (82–366 mg L −1 ) reported for other amphibian species (reviewed by Gibbons et al 44 ). The imidacloprid concentrations used in this study may nevertheless be representative of the levels of contamination found typically in surface water in agricultural areas 45 , and appear not to cause mortality but showed different chronic effects in other aquatic vertebrate species, such as R. sylvatica , exposed to 10–500 µg L −1 (cited as ppm) of the insecticide 43 , and the fish Pimephales promelas (10 µg L−1) 46 .…”

Section: Discussionmentioning

confidence: 91%

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Samojeden

Pavan

Rutkoski

et al. 2022

Sci Rep

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Imidacloprid is a neonicotinoid insecticide used to control agricultural pests around the world. This pesticide can have adverse effects on non-target organisms, especially in aquatic environments. The present study evaluated the toxicity of an imidacloprid-based insecticide in amphibians, using Leptodactylus luctator and Physalaemus cuvieri tadpoles as study models. Spawning of both species were collected within less than 24 h of oviposition from a non-agricultural land at Erechim, Rio Grande do Sul state, Brazil. Survival, swimming activity, body size, morphological malformations, and genotoxic parameters were analyzed at laboratory conditions. A short-term assay was conducted over 168 h (7 days) with five different concentrations of imidacloprid (3–300 µg L−1) being tested. The insecticide did not affect survival, although the tadpoles of both species presented reduced body size, malformed oral and intestine structures, and micronuclei and other erythrocyte nuclear abnormalities following exposure to this imidacloprid-based compound. Exposure also affected swimming activity in L. luctator, which reflected the greater sensitivity of L. luctator to imidacloprid in comparison with P. cuvieri. The swimming activity, body size, and malformations observed in L. luctator and the morphological malformations found in P. cuvieri indicated that even the lowest tested concentration of the insecticide were harmful to amphibians. At concentrations of over 3 μg L−1, P. cuvieri presents a smaller body size, and both species are affected by genotoxic cell damage. This demonstrates that imidacloprid is potentially toxic for the two study species at environmentally relevant concentrations.

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Section: Discussionmentioning

confidence: 91%

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Samojeden

Pavan

Rutkoski

et al. 2022

Sci Rep

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“…The first region contains the 5′ end of the abr gene, a general regulator of rho GTPases ( Chuang et al, 1995 ), the uncharacterized gene LOC120472185, and an aspartoacylase-encoding gene (LOC120472146). Aspartoacylase expression is a known transcriptomic biomarker of insecticide exposure in fish ( Beggel et al, 2012 ; Stinson et al, 2022 ). The second region includes two homologs of zona-pellucida sperm-binding protein 3 ( zp3 ) as well as the basal transcription factor taf6 .…”

Section: Resultsmentioning

confidence: 99%

Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas

Klymus

Hrabik

Thompson

et al. 2022

PeerJ

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Background The fathead minnow (Pimephales promelas) is a model species for toxicological research. A high-quality genome reference sequence is available, and genomic methods are increasingly used in toxicological studies of the species. However, phylogenetic relationships within the genus remain incompletely known and little population-genomic data are available for fathead minnow despite the potential effects of genetic background on toxicological responses. On the other hand, a wealth of extant samples is stored in museum collections that in principle allow fine-scale analysis of contemporary and historical genetic variation. Methods Here we use short-read shotgun resequencing to investigate sequence variation among and within Pimephales species. At the genus level, our objectives were to resolve phylogenetic relationships and identify genes with signatures of positive diversifying selection. At the species level, our objective was to evaluate the utility of archived-sample resequencing for detecting selective sweeps within fathead minnow, applied to a population introduced to the San Juan River of the southwestern United States sometime prior to 1950. Results We recovered well-supported but discordant phylogenetic topologies for nuclear and mitochondrial sequences that we hypothesize arose from mitochondrial transfer among species. The nuclear tree supported bluntnose minnow (P. notatus) as sister to fathead minnow, with the slim minnow (P. tenellus) and bullhead minnow (P. vigilax) more closely related to each other. Using multiple methods, we identified 11 genes that have diversified under positive selection within the genus. Within the San Juan River population, we identified selective-sweep regions overlapping several sets of related genes, including both genes that encode the giant sarcomere protein titin and the two genes encoding the MTORC1 complex, a key metabolic regulator. We also observed elevated polymorphism and reduced differentation among populations (FST) in genomic regions containing certain immune-gene clusters, similar to what has been reported in other taxa. Collectively, our data clarify evolutionary relationships and selective pressures within the genus and establish museum archives as a fruitful resource for characterizing genomic variation. We anticipate that large-scale resequencing will enable the detection of genetic variants associated with environmental toxicants such as heavy metals, high salinity, estrogens, and agrichemicals, which could be exploited as efficient biomarkers of exposure in natural populations.

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“…The residue was purified by column chromatography to obtain 190 mg of Hapten 1 (DCM : MeOH = 20 : 1, 29%). 1 H NMR (400 MHz, methanol-d4) δ 8.44 (dd, J = 4.7, 1.6 Hz, 1H), 8.03 (dd, J = 8.1, 1.6 Hz, 1H), 7.75 (d, J = 2.3 Hz, 1H), 7.52 (dd, J = 8.1, 4.7 Hz, 1H), 7.48 (d, J = 2.3 Hz, 1H), 7.32 (s, 1H), 3.28-3.24 (m, 2H), 2.29 (t, J = 7.3 Hz, 2H), 1.84-1.71 (m, 2H).…”

Section: Buffers and Solutionsmentioning

confidence: 99%

“…The residue was purified by column chromatography to obtain 780 mg of Hapten 2 (DCM : MeOH = 20 : 1, 65%). 1 H NMR (400 MHz, DMSO-d 6 ) δ 12.52 (s, 1H), 12.13 (s, 1H), 9.02 (s, 1H), 8.54 (dd, J = 4.7, 1.6 Hz, 1H), 8.30-8.15 (m, 2H), 7.93 (d, J = 2.4 Hz, 1H), 7.67 (dd, J = 8.1, 4.7 Hz, 1H), 7.55 (dd, J = 8.9, 2.4 Hz, 1H), 7.19 (s, 1H), 3.33 (q, J = 6.0 Hz, 2H), 2.32 (t, J = 7.3 Hz, 2H), 1.79 ( p, J = 7.2 Hz, 2H).…”

Section: Buffers and Solutionsmentioning

confidence: 99%

“…They modulate the ryanodine receptor (RyR), which mediates calcium release into the cytoplasm, resulting in the contraction, paralysis, and finally death of pests. [1][2][3][4][5] CHL, also known as Rynaxypyr™ was the first anthranilic diamide developed by DuPont Crop Protection. 6 It has been widely used for the control of insects such as moths, beetles, and caterpillars.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

Guo

Kuang

et al. 2022

Analyst

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Agricultural surface water, imidacloprid, and chlorantraniliprole result in altered gene expression and receptor activation in Pimephales promelas

Cited by 17 publications

References 58 publications

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Toxicity and genotoxicity of imidacloprid in the tadpoles of Leptodactylus luctator and Physalaemus cuvieri (Anura: Leptodactylidae)

Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas

Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

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