Agricultural applications of insect ecological genomics

Poelchau, Monica F.; Coates, Brad S.; Childers, Christopher P.; León, Adalberto A Pérez de; Evans, Jay D.; Hackett, Kevin J.; Shoemaker, DeWayne

doi:10.1016/j.cois.2015.12.002

Cited by 25 publications

(15 citation statements)

References 103 publications

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“…While still in its infancy, pest genomics promises the identification of new gene targets for pesticide development, indication of RNAi targets for overcoming resistance and a better understanding of pests genomics, leading to the development of effective control methods. Additionally, this information will allow the exploration of crop genomes for the identification of traits that may discourage pest feeding . Weed science has generally lagged in its uptake and use of new genomic tools .…”

Section: Introductionmentioning

confidence: 99%

A chromosome‐scale draft sequence of the Canada fleabane genome

Laforest

Martin

Bisaillon

et al. 2020

Pest Management Science

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Background: Due to the accessibility of underlying technologies the 'Omics', in particular genomics, are becoming commonplace in several fields of research, including the study of agricultural pests. The weed community is starting to embrace these approaches; genome sequences have been made available in the past years, with several other sequencing projects underway, as promoted by the International Weed Genome Consortium. Chromosome-scale sequences are essential to fully exploit the power of genetics and genomics.Results: We report such an assembly for Conyza canadensis, an important agricultural weed. Third-generation sequencing technology was used to create a genome assembly of 426 megabases, of which nine chromosome-scale scaffolds cover more than 98% of the entire assembled sequence. As this weed was the first to be identified with glyphosate resistance, and since we do not have a firm handle on the genetic mechanisms responsible for several herbicide resistances in the species, the genome sequence was annotated with genes known to be associated with herbicide resistance. A high number of ABC-type transporters, cytochrome P450 and glycosyltransferases (159, 352 and 181, respectively) were identified among the list of ab initio predicted genes.Conclusion: As C. canadensis has a small genome that is syntenic with other Asteraceaes, has a short life cycle and is relatively easy to cross, it has the potential to become a model weed species and, with the chromosome-scale genome sequence, contribute to a paradigm shift in the way non-target site resistance is studied.

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Section: Introductionmentioning

confidence: 99%

A chromosome‐scale draft sequence of the Canada fleabane genome

Laforest

Martin

Bisaillon

et al. 2020

Pest Management Science

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“…Note that due to space limitations Bt mode of action will not be presented, but readers are directed to several reviews on the topic [38,39,40]. Additionally, descriptions of many methods described herein are presented by [41].…”

Section: Receptor-mediated Resistancementioning

confidence: 99%

Bacillus thuringiensis toxin resistance mechanisms among Lepidoptera: progress on genomic approaches to uncover causal mutations in the European corn borer, Ostrinia nubilalis

Coates

2016

Current Opinion in Insect Science

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Transgenic plants that express Bacillus thuringiensis (Bt) crystal (Cry) protein toxins (Bt crops) effectively control feeding by the European corn borer, Ostrinia nubilalis, although documented resistance evolution among a number of species in both the laboratory and field has heightened concerns about the durability of this technology. Research has provided major insights into the mutations that alter Bt toxin binding receptor structure and function within the midgut of Lepidoptera that directly impacts the efficacy of Bt toxins, and potentially leads to the evolution of resistance to Bt crops in the field. In this manuscript we provide an overview of available data on the identification of genes involved in high levels of resistance to Cry toxins, with emphasis on resistance described for O. nubilalis as the main target of Bt corn.

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“…RNAi is a molecular mechanism that disrupts genes in a target insect prior to the construction of a critical protein, resulting in death of the insect. Double stranded RNA (dsRNA) designed to induce gene knock-down by RNAi in pest insects has been successfully demonstrated and is being developed for implementation in crop protection strategies [1]. RNAi was demonstrated targeting key beetle pests of maize, Zea mays L. (corn), the corn rootworms, and a devastating pest of potatoes, Solanum tuberosum L. the Colorado potato beetle (CBP), Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) [2].…”

Section: Introductionmentioning

confidence: 99%

Comparison of RNAi Sequences in Insect-Resistant Plants to Expressed Sequences of a Beneficial Lady Beetle: A Closer Look at Off-Target Considerations

Allen¹

2017

Insects

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Sequences obtained from transcriptomes of the lady beetle Coleomegilla maculata were compared to those designed for incorporation into crops. Searches of the transcriptomes identified sequences as the most likely to be closely similar to the sequences described in RNAi plant incorporated products. Some proposed prime RNAi pest management targets were also used to identify predicted orthologs from C. maculata. The lady beetle sequences were aligned with sequences from corn rootworms and Colorado potato beetles and, as appropriate in the case of targets, regions of similarity were compared with the genetic model organism for beetles, Tribolium castaneum. Some high levels of nucleotide identity were identified, particularly with an actin-derived sequence from Colorado potato beetle. This actin-derived sequence shared identical sequences with the lady beetle and a parasitic wasp.

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Agricultural applications of insect ecological genomics

Cited by 25 publications

References 103 publications

A chromosome‐scale draft sequence of the Canada fleabane genome

A chromosome‐scale draft sequence of the Canada fleabane genome

Bacillus thuringiensis toxin resistance mechanisms among Lepidoptera: progress on genomic approaches to uncover causal mutations in the European corn borer, Ostrinia nubilalis

Comparison of RNAi Sequences in Insect-Resistant Plants to Expressed Sequences of a Beneficial Lady Beetle: A Closer Look at Off-Target Considerations

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