Bacillus thuringiensis toxin resistance mechanisms among Lepidoptera: progress on genomic approaches to uncover causal mutations in the European corn borer, Ostrinia nubilalis

Coates, Brad S.

doi:10.1016/j.cois.2016.04.003

Cited by 13 publications

(10 citation statements)

References 83 publications

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“…Everything that we know today about the mechanism of action of 3D-toxins has been mainly obtained from two sources of information: the construction of 3D-Cry mutants and the study of the resistance phenomena that insects have shown to the action of 3D-Cry toxins [24,25]. The study of mutant proteins is one of the pillars for the elucidation of any mechanism of action of any protein.…”

Section: Introductionmentioning

confidence: 99%

Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action

Vı́lchez

2020

Toxins

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3D-Cry toxins, produced by the entomopathogenic bacterium Bacillus thuringiensis, have been extensively mutated in order to elucidate their elegant and complex mechanism of action necessary to kill susceptible insects. Together with the study of the resistant insects, 3D-Cry toxin mutants represent one of the pillars to understanding how these toxins exert their activity on their host. The principle is simple, if an amino acid is involved and essential in the mechanism of action, when substituted, the activity of the toxin will be diminished. However, some of the constructed 3D-Cry toxin mutants have shown an enhanced activity against their target insects compared to the parental toxins, suggesting that it is possible to produce novel versions of the natural toxins with an improved performance in the laboratory. In this report, all mutants with an enhanced activity obtained by accident in mutagenesis studies, together with all the variants obtained by rational design or by directed mutagenesis, were compiled. A description of the improved mutants was made considering their historical context and the parallel development of the protein engineering techniques that have been used to obtain them. This report demonstrates that artificial 3D-Cry toxins made in laboratories are a real alternative to natural toxins.

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Section: Introductionmentioning

confidence: 99%

Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action

Vı́lchez

2020

Toxins

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“…Within the model species for Coleoptera, the red flour beetle, Tribolium castaneum, Broehan et al [30] reported that RNAimediated knockdown of some ABC transporters resulted in mortality, or phenotypes characterized by arrested growth, abnormal cuticle formation, defective eye pigmentation, or abnormal egg-laying orhatching. Changes in the expression level or structure of some ABCA, ABCC and ABCG subfamily members have been associated with Bt toxin resistance in species of Lepidoptera [37], while paralogs of an ABCB transporter were linked to Cry3Aa resistance in the coleopteran species, Chrysomela tremula [38], and were found to be in proximity to a quantitative trait locus (QTL) for Cry3Bb1 resistance in D. v. virgifera [39].…”

Section: Introductionmentioning

confidence: 99%

Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family

Adedipe

Grubbs

Coates

et al. 2019

Preprint

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Background The western corn rootworm, Diabrotica virgifera virgifera , is a pervasive pest of cultivated maize in North America and Europe, which has adapted to survive exposure to multiple insecticidal agents. Due to their role in insecticide transport, we sought to identify members of the ATP-binding cassette (ABC) transporter family in D. v. virgifera using a transcriptomics approach.Results In this study, 65 putative D. v. virgifera ABC ( Dvv ABC) transporters were identified within a combined transcriptome assembly generated from embryonic, larval, adult male, and adult female RNA-sequence libraries. Phylogenetic analysis placed the deduced amino-acid sequences of the Dvv ABC transporters into eight subfamilies (A to H). Of these, eight shared structural and functional conservation with Tribolium castaneum ABC transporter orthologs known to exhibit overt RNA interference (RNAi) knockdown phenotypes. Interestingly, depletion of DvvABCB_19147 and DvvABCG_3712 transcripts in adult females produced detrimental reproductive and developmental phenotypes (egg-laying or -hatching defects), demonstrating the potential of these genes as targets for RNAi-mediated insect control tactics.Conclusions By combining sequence data from four libraries covering three distinct life stages, we have produced a relatively comprehensive de novo transcriptome assembly for D. v. virgifera . Moreover, we have identified 65 members of the ABC transporter family, and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species.

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“…Within the model species for Coleoptera, the red flour beetle, Tribolium castaneum, Broehan et al [30] reported that RNAi-mediated knockdown of some ABC transporters resulted in mortality, or phenotypes characterized by arrested growth, abnormal cuticle formation, defective eye pigmentation, or abnormal egg-laying or -hatching. Changes in the expression level or structure of some ABCA, ABCC and ABCG subfamily members are associated with Bt toxin resistance in species of Lepidoptera [38].…”

Section: Introductionmentioning

confidence: 99%

Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family

Adedipe

Grubbs

Coates³

et al. 2019

Preprint

View full text Add to dashboard Cite

Background The western corn rootworm, Diabrotica virgifera virgifera, is a pervasive pest of cultivated maize in North America and Europe, which has adapted to survive exposure to multiple insecticidal agents. Due to their role in insecticide transport, we sought to identify members of the ATP-binding cassette (ABC) transporter family in D. v. virgifera using a transcriptomics approach. Results In this study, 65 putative D. v. virgifera ABC (DvvABC) transporters were identified within a combined transcriptome assembly generated from embryo, larval, adult male, and adult female RNA-sequence libraries. Phylogenetic analysis placed the deduced amino-acid sequences of the DvvABC transporters into eight subfamilies (A to H). Of these, eight shared structural and functional conservation with Tribolium castaneum ABC transporter orthologs known to exhibit overt RNA interference (RNAi) knockdown phenotypes. Interestingly, depletion of DvvABCB_19147 and DvvABCG_3712 transcripts in adult females produced detrimental reproductive and developmental phenotypes (egg-laying or -hatching defects), demonstrating the potential of these genes as targets for RNAi-mediated insect control tactics. Conclusions By combining sequence data from four libraries covering three distinct life stages, we have produced a relatively comprehensive de novo transcriptome assembly for D. v. virgifera. Moreover, we have identified 65 members of the ABC transporter family, and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species.

show abstract

Bacillus thuringiensis toxin resistance mechanisms among Lepidoptera: progress on genomic approaches to uncover causal mutations in the European corn borer, Ostrinia nubilalis

Cited by 13 publications

References 83 publications

Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action

Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action

Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family

Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family

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