Agr typing of Staphylococcus aureus species isolated from clinical samples in training hospitals of Isfahan and Shahrekord

Javdan, Saeid; Narimani, Tahmine; Abadi, Milad Shahini Shams; Gholipour, Abolfazl

doi:10.1186/s13104-019-4396-8

Cited by 21 publications

(15 citation statements)

References 17 publications

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“…More than 75% of the isolates belonged to this type. Predominance of agr type 1 is in accordance with previous data from Iran [11][12] . In the phenotypic assay, 12 of 85 isolates tested were mupirocin resistant.…”

Section: Discussionsupporting

confidence: 91%

“…The agr operon includes agrA, agrB, agrC, and agrD genes. Agr system is polymorphic and permits the classification of S. aureus strains into four groups (agr I, agr II, agr III, and agr IV) according to sequences diversity in variable regions, comprising the last third of agrB and agrD as well as the first half of agrC genes [11][12] . Objectives: The aim of this study was to evaluate agr types and mupirocin resistance in clinical isolates of S. aureus in Guilan province.…”

Section: Introductionmentioning

confidence: 99%

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Agr Typing and Detection of Mupirocin Resistance in Multi-Drug Resistant Clinical Isolates of Staphylococcus aureus in Northern Iran

Asadpour

Jafarnejhad

Abdi

2021

IEM

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Background: Mupirocin is a topical antibiotic inhibiting most Gram-positive cocci. Shortly after taking mupirocin, drug resistance emerges. This study aimed to determine mupirocin resistance in Staphylococcus aureus strains isolated from clinical specimens in Rasht. Materials & Methods: In this study, a total of 85 clinical isolates of S. aureus were collected. Biofilm formation ability and antibacterial resistance patterns of isolates were investigated. Disc diffusion method and MIC determination were used to determine the susceptibility of strains to mupirocin antibiotic. Agr types, the presence of mupA, and mutation in ileS-1 were evaluated in mupirocin non-susceptible isolates by PCR and PCR sequencing, respectively. Findings: Out of 85 tested strains, 57 (67%) isolates were recognized as biofilm producers, and all of which showed multidrug resistance phenotype. Agr type 1 was the most commonly detected type. Additionally, 12 mupirocin-resistant strains were identified in the disc diffusion and MIC tests. A total of four strains were mup-A positive and showed high-level resistance. In sequencing and mutation evaluation of the ileS-1 gene in eight low-level mupirocin-resistant strains, 12 types of silent mutation and one type of missense mutation were determined. Conclusion:The study of mupirocin-resistant strains in this study showed the need to identify factors affecting the occurrence of resistance and to take control and prevention measures before mupirocin losses its efficacy.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Agr Typing and Detection of Mupirocin Resistance in Multi-Drug Resistant Clinical Isolates of Staphylococcus aureus in Northern Iran

Asadpour

Jafarnejhad

Abdi

2021

IEM

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“…Our agr III isolates showed more antibiotic susceptibility than agr I isolates. A study in Pakistan showed that agr III isolates were more resistant than agr I isolates [ 32 ]; whereas, in Iran, a significant relation was found between agr I and resistance to cefoxitin and erythromycin ( P < 0.05) [ 33 ]. In addition, in our study, agr III isolates more commonly carried the mentioned virulence genes compared to agr I isolates; but the sea was only found in the agr I isolates (Fig.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic susceptibility of human-associated Staphylococcus aureus and its relation to agr typing, virulence genes, and biofilm formation

2021

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Background and objective Carriage of virulence factors confers some evolutionary benefit to bacteria, which favors the resistant strains. We aimed to analyze whether antibiotic susceptibility of Staphylococcus aureus strains is affected by agr typing, biofilm formation ability, and virulence profiles. Methods A total of 123 S. aureus clinical isolates were subjected to antimicrobial susceptibility testing by disk diffusion method, biofilm formation by microtiter plate method, as well as polymerase chain reaction screening to identify virulence genes and the accessory gene regulator (agr) types I-IV. A P value < 0.05 was considered significant. Results The most prevalent virulence gene was staphyloxanthin crtN, followed by hemolysin genes, capsular cap8H, toxic shock toxin tst, and enterotoxin sea, respectively. Resistant isolates were more commonly found in the agr-negative group than in the agr-positive group. Isolates of agr type III were more virulent than agr I isolates. Strong biofilm producers showed more antibiotic susceptibility and carried more virulence genes than non-strong biofilm producers. Associations were found between the presence of virulence genes and susceptibility to antibiotics. Carriage of the virulence genes and agr was higher in the inpatients; while, resistance and strong biofilms were more prevalent in the outpatients. Conclusion These findings indicated the presence of several virulence factors, biofilm production capacity, agr types and resistance to antibiotics in clinical S. aureus isolates. Considering the importance of S. aureus for human medicine, an understanding of virulence and resistance relationships would help to reduce the impact of S. aureus infections.

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“…According to Figures-8 to 11, our results showed that agrI was the most common type found in isolated S. aureus. Javdan et al [40], and Cheraghi et al [41], stated that the most dominant type was agr Type I. It is said that definite groups of agr in S. aureus are implicated in certain diseases, such as isolates that possess agrI Available at www.veterinaryworld.org/Vol.15/January-2022/26.pdf are related to bacteremia and persistent diseases [42].…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of genes responsible for biofilm formation in Staphylococcus aureus isolated from mastitic cows

et al. 2022

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Background and Aim: Mastitis is considered a significant disease of lactating animals. There are new attitudes for recognizing genes responsible for causing this disease to overcome and change the manipulation of this problem. This study aimed to isolate and identify Staphylococcus aureus strains from mastitic bovine animals and detect some specific biofilm-forming genes (icaA, icaD, and biofilm-associated protein [bap] genes clfA, fnbA, agrI, agrII, agrIII, agrIV, and cna). Materials and Methods: A total of 121 mastitic milk samples were analyzed using biochemical tests (catalase test, oxidative-fermentative test, and coagulase test) and Gram stain. Multiplex polymerase chain reaction was applied to characterize biofilm genes (icaA, icaD, bap, clfA, and fnbA) in addition to (agrI, agrII, agrIII, agrIV, and cna). Results: Among the 121 milk samples, 35 staphylococci isolates were derived with an incidence of 28.92% (35/121); among them, 19 are coagulase positive. Ninety percent of the isolates had ica genes (icaA and icaD) while bap gene was not recognized in any isolate. In addition, the incidence of fnbA, can, and clfA was 89.5% each. The prevalence of agr specific groups (agrI, agrII, agrIII, and agrIV) was 78.9%, 52.6%, 10.5%, and 15.8%, respectively. Conclusion: This study concluded that S. aureus has variant mechanisms of pathogenicity to form biofilm devoid of carrying a specific gene.

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Agr typing of Staphylococcus aureus species isolated from clinical samples in training hospitals of Isfahan and Shahrekord

Cited by 21 publications

References 17 publications

Agr Typing and Detection of Mupirocin Resistance in Multi-Drug Resistant Clinical Isolates of Staphylococcus aureus in Northern Iran

Agr Typing and Detection of Mupirocin Resistance in Multi-Drug Resistant Clinical Isolates of Staphylococcus aureus in Northern Iran

Antibiotic susceptibility of human-associated Staphylococcus aureus and its relation to agr typing, virulence genes, and biofilm formation

Molecular characterization of genes responsible for biofilm formation in Staphylococcus aureus isolated from mastitic cows

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