Molecular characterization of genes responsible for biofilm formation in Staphylococcus aureus isolated from mastitic cows

Ibrahim, E. S.; Arafa, Amany A.; Dorgham, Sohad M.; Eid, Rasha Hamdy; Ata, Nagwa S.; El-Dabae, Wahid; Gaber, E. S.

doi:10.14202/vetworld.2022.205-212

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…In the current study, the S. aureus isolates exhibited high prevalence of fnbpA , clfA , clfB , sdrC , cna , and sdrE . Similar observations have also been reported in our previous study and by other reports ( Yang et al, 2020 ; Avila-Novoa et al, 2022 ; Ibrahim et al, 2022 ). However, the frequencies of ebpS , sdrD , and map / eap found in our study was lower than those reported by other authors ( Cheraghi et al, 2017 ; Kot et al, 2022 ).…”

Section: Discussionsupporting

confidence: 93%

Antimicrobial resistance and virulence profiles of staphylococci isolated from clinical bovine mastitis

Yang

Meng

et al. 2023

Front. Microbiol.

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Staphylococci, mainly including Staphylococcus aureus and coagulase-negative staphylococci (CNS), are one of the most common pathogens causing bovine mastitis worldwide. In this study, we investigated the antimicrobial resistance and virulence profiles of staphylococci from clinical bovine mastitis in Ningxia Hui Autonomous Region of China. Antimicrobial resistance was determined by disc diffusion combined with E-test method. Genes of antimicrobial resistance and virulence factors were determined by PCR. A total of 332 staphylococcal isolates were confirmed from 1,519 mastitic milk samples, including 172 S. aureus and 160 CNS isolates. Fifteen CNS species were identified, with S. chromogenes being the most frequent found (49.4%), followed by S. equorum (13.8%). Noticeably, 2 S. agnetis isolates were found among the CNS isolates. To our knowledge, this is the first report documenting the presence of S. agnetis from bovine mastitis in China. The S. aureus and CNS isolates showed high resistance against penicillin, followed by erythromycin and tetracycline. Multidrug resistance was found in 11.6 and 16.3% of the S. aureus and CNS isolates, respectively. Resistance to penicillin was attributed to the presence of blaZ, erythromycin resistance to ermC (alone or combined with ermB) and tetracycline resistance to tetK (alone or combined with tetM). Notably, one S. equorum isolate and one S. saprophyticus isolate were both methicillin-resistant and mecA positive. Additionally, all S. aureus isolates carried the adhesin genes fnbpA, clfA, clfB, and sdrC, and most of them contained cna and sdrE. Conversely, only a few of the CNS isolates carried clfA, cna, and fnbA. Regarding toxin genes, all S. aureus isolates harbored hlb, and most of them were hlg positive. The lukE-lukD, lukM, sec, sed, sei, sen, seo, tst, seg, seh, and sej were also detected with low frequencies. However, no toxin genes were observed in CNS isolates. This study reveals high species diversity of staphylococci from clinical bovine mastitis in Ningxia Hui Autonomous Region of China. The findings for the genetic determinants of antimicrobial resistance and virulence factor provide valuable information for control and prevention of staphylococcal bovine mastitis.

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Section: Discussionsupporting

confidence: 93%

Antimicrobial resistance and virulence profiles of staphylococci isolated from clinical bovine mastitis

Yang

Meng

et al. 2023

Front. Microbiol.

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“…In the current study, the S. aureus isolates exhibited high prevalence of fnbpA, clfA, clfB, sdrC, cna, and sdrE. Similar observations have also been reported in our previous study and by other reports Avila-Novoa et al, 2022;Ibrahim et al, 2022). However, the frequencies of ebpS, sdrD, and map/eap found in our study was lower than those reported by other authors (Cheraghi et al, 2017;Kot et al, 2022).…”

Section: Discussionsupporting

confidence: 89%

One-Health meets microbiota: Interactions between digestive tract microbiota, host, and environment

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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“…Livestock is the major reservoir of MRSA infection, with specifc genotypic and phenotypic traits of 3% S. aureus infection [38][39][40]. Te prevalence of S. aureus associated with intramammary infections in CM and SCM mastitis in dairy animals was 25.3% as shown in Tables 3 and 4, while other studies in Egypt (35.9%) [41,42], and disagree with the lower prevalence (6.5%) reported by [40,43,44].…”

Section: Discussionmentioning

confidence: 78%

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One of the Alternative Treatments

Abdalhamed

Zeedan

Arafa

et al. 2022

Veterinary Medicine International

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Mastitis is an important disease in dairy animals worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of clinical and subclinical intramammary infections. In the current study, we isolated bacteria from 150 mastitic milk samples. Multiplex PCR was used to detect the methicillin resistance genes in S. aureus to detect the occurrence of MRSA isolates. Green synthesized titanium dioxide nanoparticles (TiO2 NPs) using aqueous leaf extracts of Artemisia herb Alba (A. herb Alba TiO2 NPs). The antibacterial efficacy of these nanoparticles was evaluated (in vitro and in vivo) against collected MRSA isolates using the disc diffusion method and SPF rats. Out of 150 mastitic milk samples, the frequency of S. aureus was 38 (25.3%), that of E. coli was 45 (30%), that of Klebsiella spp. Was 7 (4.7%), and that of Streptococcus spp. Was 11 (7.3%). Among 38 positive isolates of S. aureus, MRSA was 16 (42.1%) by antimicrobial sensitivity testing (AST) and 14 (38.8%) by multiplex PCR. The MRSA isolates were shown to have 100% resistance to penicillin and methicillin, 87.5% resistance to gentamicin, 50% resistance to cefoxitin and amoxicillin, and 75% resistance to ampicillin and ampicillin/sublactam with low resistance against erythromycin, ciprofloxacin, tetracycline, and levofloxacin by AST, respectively. A. herb Alba TiO2 NP formation was observed by changing the colour from white to dark green. The UV spectrum revealed absorbance peaks at 240–250 nm, and their sizes ranged from 42–66 nm and 11 to 45 nm by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). ‏A. herb Alba TiO2 NP suspensions were evaluated against MRSA, with the highest zone of inhibition (43 ± 0.45 mm) at a concentration of 40 μg/ml. Hematological parameters and histological examination after oral administration of 20 mg/kg of A. herb Alba TiO2 NPs indicated that A. herb Alba TiO2 NPs can be used as a new antimicrobial against resistant bacteria (MRSA) with consideration of the dose and methods of synthesis of plant-based compounds.

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Molecular characterization of genes responsible for biofilm formation in Staphylococcus aureus isolated from mastitic cows

Cited by 7 publications

References 38 publications

Antimicrobial resistance and virulence profiles of staphylococci isolated from clinical bovine mastitis

Antimicrobial resistance and virulence profiles of staphylococci isolated from clinical bovine mastitis

One-Health meets microbiota: Interactions between digestive tract microbiota, host, and environment

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One of the Alternative Treatments

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