Aglycone and glycoside specificity of apple skin flavonoid glycosyltransferase

Lister, Carolyn E.; Lancaster, Jane E.; Sutton, Kevin H.; Walker, John R.

doi:10.1002/(sici)1097-0010(199711)75:3<378::aid-jsfa887>3.0.co;2-z

Cited by 17 publications

(3 citation statements)

References 11 publications

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“…The other point at which substrate competition may occur is glycosylation of quercetin, which provides stability to flavonol compounds. Lister et al (1997) showed the enzyme MdUFGT can catalyse the glycosylation of both cyanidin and quercetin, but had twice the relative activity with cyanidin than with quercetin as substrate. Therefore, substrate competition in vivo could favour the anthocyanin pathway.…”

Section: Discussionmentioning

confidence: 93%

Transcriptional regulation of the flavonoid pathway in the skin of dark-grown ‘Cripps’ Red’ apples in response to sunlight

Takos

Robinson

Walker

2006

The Journal of Horticultural Science and Biotechnology

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Section: Discussionmentioning

confidence: 93%

Transcriptional regulation of the flavonoid pathway in the skin of dark-grown ‘Cripps’ Red’ apples in response to sunlight

Takos

Robinson

Walker

2006

The Journal of Horticultural Science and Biotechnology

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“…The different gene expression and enzyme activity responses might be related to the different cultivar properties, as ‘Golden Delicious’ is a non-red cultivar and ‘Fuji’ is a red cultivar. UFGT catalyzes the glycosylation of both cyanidin and quercetin in apple peel [27,40], while DFR is only involved in the synthesis of cyanidin [30,31,32]. Therefore, under relatively high sunlight conditions, the lower concentrations of quercetin-3-glycoside in DPI-treated ‘Fuji’ peels suggest that DPI may inhibit the synthesis of anthocyanin mainly by affecting the activity of UFGT (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

Chen

Zhang

et al. 2019

IJMS

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Sunlight radiation is a main environmental factor which affects anthocyanin synthesis. To clarify the regulatory mechanism of sunlight on the synthesis of anthocyanin in apple peel, bagged apples were exposed to diverse intensities of sunlight through different shading treatments. Under an increased solar ultraviolet-B (UV-B) light intensity, the concentration of anthocyanin in apple peels was consistent with the Michaelis–Menten equation. Under lower sunlight intensities, diphenyleneiodonium chloride (DPI, an inhibitor of plasma membrane NAD(P)H oxidase) treatment increased both the concentration of cyanidin-3-glycoside and the activity of dihydroflavonol 4-reductase (DFR). However, under higher sunlight intensities, DPI treatment decreased the concentrations of cyanidin-3-glycoside and quercetin-3-glycoside, as well as the activities of DFR and UDP-glycose: flavonoid 3-O-glycosyltransferase (UFGT). These results indicate that, under low sunlight intensity, anthocyanin synthesis in apple peel was limited by the supply of the substrate cyanidin, which was regulated by the DFR activity. Nevertheless, after exposure to high sunlight intensity, the anthocyanin produced in the apple peel was dependent on UFGT activity.

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“…As UFGT catalyzes the transfer of glycosyl moieties from nucleotide sugar donors to the hydroxyl group of anthocyanidins and other flavonoid acceptors such as quercetin or isorhamnetin (Ju et al , Lister et al ), it is not surprising that the peel of red ‘Anjou’ also had higher concentrations of quercetin‐3‐glycoside and isorhamnetin‐3‐glycoside (Fig. ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of phenolic metabolism and primary metabolism between green ‘Anjou’ pear and its bud mutation, red ‘Anjou’

Zhang

Einhorn

et al. 2013

Physiologia Plantarum

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Green 'Anjou' pear and its bud mutation, red 'Anjou' were compared to understand their differences in phenolic metabolism and its effect on primary metabolism. In the flesh of the two cultivars, no difference was detected in the concentration of any phenolic compound, the transcript level of MYB10 or the transcript levels or activities of key enzymes involved in anthocyanin synthesis. Compared with green 'Anjou', the shaded peel of red 'Anjou' had higher anthocyanin concentrations, higher transcript levels of MYB10 and higher activity of UDP-glucose:flavonoid 3-O-glycosyltransferase (UFGT), suggesting that MYB10 regulates UFGT to control anthocyanin synthesis in red 'Anjou' peel. In the sun-exposed peel, activities of phenylalanine ammonia lyase, dihydroflavonol reductase, flavonol synthase and anthocyanidin synthase as well as UFGT were higher in red 'Anjou' than in green 'Anjou'. The peel of red 'Anjou' had higher activities of sorbitol dehydrogenase, raffinose synthase and sucrose synthase and higher levels of raffinose, myo-inositol and starch, indicating that sorbitol metabolism, raffinose synthesis and starch synthesis were upregulated in red 'Anjou'. The flesh of red 'Anjou' had higher concentrations of glucose, but lower activities of ATP-dependent phosphofructokinase, pyruvate kinase and glucose-6-phosphate dehydrogenase and lower dark respiration. The peel of red 'Anjou' had higher activities of glutaminase, asparagine synthetase and asparaginase, and higher concentrations of asparagine, aspartate, alanine, valine, threonine and isoleucine. The effects of anthocyanin synthesis on primary metabolism in fruit peel are discussed.

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Aglycone and glycoside specificity of apple skin flavonoid glycosyltransferase

Cited by 17 publications

References 11 publications

Transcriptional regulation of the flavonoid pathway in the skin of dark-grown ‘Cripps’ Red’ apples in response to sunlight

Transcriptional regulation of the flavonoid pathway in the skin of dark-grown ‘Cripps’ Red’ apples in response to sunlight

Differential Regulation of Anthocyanin Synthesis in Apple Peel under Different Sunlight Intensities

Comparison of phenolic metabolism and primary metabolism between green ‘Anjou’ pear and its bud mutation, red ‘Anjou’

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