Aging of a Regenerative Biologic Scaffold (AlloDerm Native Tissue Matrix) During Storage at Elevated Humidity and Temperature

Sun, Wendell Q.; Gouk, Sok-Siam

doi:10.1089/ten.tec.2008.0164

Cited by 13 publications

(2 citation statements)

References 34 publications

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“…A naturally derived dermis traded as AlloDerm  (LifeCell Corporation) has a porous and fibrillar structure similar to native dermis prepared using lyophilization technique after decellularization treatment as shown in Figure 1A and B. 20,21 AlloDerm has been used as autograft in the resurfacing of burn wound reconstruction due to its human origin. 22 Another acellular natural product (Integra  , Integra Life Sciences) was engineered using a porous composite of bovine collagen-chondroitin-6-sulfate (8 wt%) with an outer silicone covering.…”

Section: Collagen-based Matricesmentioning

confidence: 99%

Tissue scaffolds for skin wound healing and dermal reconstruction

Zhong

Zhang

Lim

2010

WIREs Nanomed Nanobiotechnol

553

409

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One of the major applications of tissue-engineered skin substitutes for wound healing is to promote the healing of cutaneous wounds. In this respect, many important clinical milestones have been reached in the past decades. However, currently available skin substitutes for wound healing often suffer from a range of problems including wound contraction, scar formation, and poor integration with host tissue. Engineering skin substitutes by tissue engineering approach has relied upon the creation of three-dimensional scaffolds as extracellular matrix (ECM) analog to guide cell adhesion, growth, and differentiation to form skin-functional and structural tissue. The three-dimensional scaffolds can not only cover wound and give a physical barrier against external infection as wound dressing, but also can provide support both for dermal fibroblasts and the overlying keratinocytes for skin tissue engineering. A successful tissue scaffold should exhibit appropriate physical and mechanical characteristics and provide an appropriate surface chemistry and nano and microstructures to facilitate cellular attachment, proliferation, and differentiation. A variety of scaffolds have been fabricated based on materials ranging from naturally occurring ones to those manufactured synthetically. This review discusses a variety of commercial or laboratory-engineered skin substitutes for wound healing. Central to the discussion are the scaffolds/materials, fabrication techniques, and their characteristics associated with wound healing. One specifically highlighted emerging fabrication technique is electrospinning that allows the design and fabrication of biomimetic scaffolds that offer tremendous potential applications in wound healing of skin.

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Section: Collagen-based Matricesmentioning

confidence: 99%

Tissue scaffolds for skin wound healing and dermal reconstruction

Zhong

Zhang

Lim

2010

WIREs Nanomed Nanobiotechnol

553

409

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“…The decrease in thermal stability of scaffolds correlates with the increased vulnerability to enzyme degradation. 15 , 49 , 50 Significant differences exist in the susceptibility to collagenase among three scaffold products ( Figure 6 ). Strattice has similar susceptibility to collagenase as fresh porcine dermis and minimally processed dermal ECM do.…”

Section: Discussionmentioning

confidence: 99%

Process-induced extracellular matrix alterations affect the mechanisms of soft tissue repair and regeneration

Sun

Sandor

et al. 2013

J Tissue Eng

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Extracellular matrices derived from animal tissues for human tissue repairs are processed by various methods of physical, chemical, or enzymatic decellularization, viral inactivation, and terminal sterilization. The mechanisms of action in tissue repair vary among bioscaffolds and are suggested to be associated with process-induced extracellular matrix modifications. We compared three non-cross-linked, commercially available extracellular matrix scaffolds (Strattice, Veritas, and XenMatrix), and correlated extracellular matrix alterations to in vivo biological responses upon implantation in non-human primates. Structural evaluation showed significant differences in retaining native tissue extracellular matrix histology and ultrastructural features among bioscaffolds. Tissue processing may cause both the condensation of collagen fibers and fragmentation or separation of collagen bundles. Calorimetric analysis showed significant differences in the stability of bioscaffolds. The intrinsic denaturation temperature was measured to be 51°C, 38°C, and 44°C for Strattice, Veritas, and XenMatrix, respectively, demonstrating more extracellular matrix modifications in the Veritas and XenMatrix scaffolds. Consequently, the susceptibility to collagenase degradation was increased in Veritas and XenMatrix when compared to their respective source tissues. Using a non-human primate model, three bioscaffolds were found to elicit different biological responses, have distinct mechanisms of action, and yield various outcomes of tissue repair. Strattice permitted cell repopulation and was remodeled over 6 months. Veritas was unstable at body temperature, resulting in rapid absorption with moderate inflammation. XenMatrix caused severe inflammation and sustained immune reactions. This study demonstrates that extracellular matrix alterations significantly affect biological responses in soft tissue repair and regeneration. The data offer useful insights into the rational design of extracellular matrix products and bioscaffolds of tissue engineering.

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