Aging-dependent modification of lipid composition and lipid structural order parameter of hepatic mitochondria

Vorbeck, Marie L.; Martin, A. J. P.; Long, James W.; Smith, Jennie M.; Orr, Richard R.

doi:10.1016/0003-9861(82)90511-2

Cited by 66 publications

(16 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Age-dependent changes in biological membranes include decreases in the membrane fluidity due to an increase of cholesterol content in the membrane and increased levels of lipid peroxidation products [Vorbeck et al, 1982;Cohen and Zubenko, 1985;Choe et al, 1995;Choi and Yu, 1995]. Our findings, although obtained using pharmacological concentrations of ML, may be consistent with the decreases observed in hepatic microsomal membrane fluidity in pinealectomized old rats compared to agematched control animals .…”

Section: Discussionsupporting

confidence: 89%

5-methoxytryptophol preserves hepatic microsomal membrane fluidity during oxidative stress

et al. 2000

View full text Add to dashboard Cite

Lipid peroxidation is a degenerative chain reaction in biological membranes that may be initiated by exposure to free radicals. This process is associated with changes in the membrane fluidity and loss of several cell membrane-dependent functions. 5-methoxytryptophol (ML) is an indole isolated from the mammalian pineal gland. The purpose of this study was to investigate the effects of ML (0. 01mM-10mM) on membrane fluidity modulated by lipid peroxidation. Hepatic microsomes obtained from rats were incubated with or without ML (0.01-10 mM). Then lipid peroxidation was induced by FeCl(3), ADP, and NADPH. Membrane fluidity was determined using fluorescence spectroscopy. Malonaldehyde (MDA) +4-hydroxyalkenals (4-HDA) concentrations were estimated as an indicator of the degree of lipid peroxidation. With oxidative stress, membrane fluidity decreased and MDA+4-HDA levels increased. ML (0.01-3 mM) reduced membrane rigidity and the rise in MDA+4-HDA formation in a concentration-dependent manner. 10 mM ML protected against lipid peroxidation but failed to prevent the membrane rigidity. In the absence of oxidative reagents, ML (0.3-10 mM) decreased membrane fluidity whereas MDA+4-HDA levels remained unchanged. This indicates that ML may interact with membrane lipids. The results presented here suggest that ML may be another pineal indoleamine (in addition to melatonin) that resists membrane rigidity due to lipid peroxidation.

show abstract

Section: Discussionsupporting

confidence: 89%

5-methoxytryptophol preserves hepatic microsomal membrane fluidity during oxidative stress

et al. 2000

View full text Add to dashboard Cite

show abstract

“…On the other hand, in cerebrospinal fluid, total F 2 -isoprostanes were higher in demented patients [68]. Lipid peroxidation cause alterations in membranes [69][70][71][72]: in a study on mitochondria extracted from different cerebral areas we found a significant reduction of membrane fluidity compared to age-matched normal subjects; it was also related to the increase in 8-OHdG content in mtDNA [73].…”

Section: Alzheimer's Diseasementioning

confidence: 84%

Oxidative stress in brain aging, neurodegenerative and vascular diseases: An overview

Mariani

Polidori

Cherubini

et al. 2005

Journal of Chromatography B

604

418

View full text Add to dashboard Cite

“…Cardiolipin is a phospholipid that is almost exclusively located to mitochondria and acts as an important cofactor of several IMM proteins [58, 59]. Studies using brain [68], liver [91], and cardiac mitochondria [6-8, 92-94], and our own work with isolated rat hepatocytes [86, 95] indicate that cardiolipin significantly declines with age. However, the extent of cardiolipin loss and/or whether such declines are functionally consequential is controversial.…”

Section: Age-associated Changes Of the Mitochondrial Energy Transdmentioning

confidence: 99%

Section: Age-associated Changes Of the Mitochondrial Energy Transdmentioning

confidence: 99%

“…phosphatidylethanolamine) [6-8, 91], the overall IMM composition and its fluidity are significantly changed with age. Cholesterol accumulates in the IMM, alters membrane fluidity, and may promote greater H + leakage [7, 91]. In a similar vein, using LC-MS/MS analysis, we recently demonstrated that aged cardiac mitochondria accumulate ceramide, a pro-apoptotic sphingolipid, which results in inhibition of complex IV activity [79].…”

Section: Age-associated Changes Of the Mitochondrial Energy Transdmentioning

confidence: 99%

See 1 more Smart Citation

Age-related decline in mitochondrial bioenergetics: Does supercomplex destabilization determine lower oxidative capacity and higher superoxide production?

Gómez

Hagen

2012

Seminars in Cell & Developmental Biology

View full text Add to dashboard Cite

Aging-dependent modification of lipid composition and lipid structural order parameter of hepatic mitochondria

Cited by 66 publications

References 48 publications

5-methoxytryptophol preserves hepatic microsomal membrane fluidity during oxidative stress

5-methoxytryptophol preserves hepatic microsomal membrane fluidity during oxidative stress

Oxidative stress in brain aging, neurodegenerative and vascular diseases: An overview

Age-related decline in mitochondrial bioenergetics: Does supercomplex destabilization determine lower oxidative capacity and higher superoxide production?

Contact Info

Product

Resources

About