Aging Changes in Ultrastructure and Enzymatic Activity of Articular Cartilage of Guinea Pigs

Silberberg, Ruth; Stamp, Warren G.; Lesker, Peggy A.; Hasler, Mary

doi:10.1093/geronj/25.3.184

Cited by 34 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Old guinea pigs displayed enhanced activity of the enzymes concerned in energy metabolism when the biochemically determined activities were calcu lated on the basis of DNA levels (56). This observation is not at variance with our results, since it was probably related to the cell proliferation described in the same study.…”

Section: Discussionsupporting

confidence: 77%

Age-Related Histochemical and Histological Changes in the Knee-Joint Cartilage of C57B1 Mice and Their Significance for the Pathogenesis of Osteoarthrosis

Pataki¹,

Rüttner²,

Abt³

1980

Pathobiology

View full text Add to dashboard Cite

For a better understanding of the part played by the aging of cartilage in the pathogenesis of osteoarthrosis, we attempted to detect functional age-related changes in the knee-joint cartilage of C57B1 mice using histochemical methods and to correlate these changes with the concomitant histological changes. A comparison of 57- and 97-week-old mice revealed a statistically significant age-related focal loss of activity for a number of oxidative enzymes of the chondrocytes. There was an associated significant increase in regressive changes of the chondrocytes. A relation probably exists between these two changes, inasmuch as the focal loss of enzyme activity precedes and possibly gives rise to the regressive changes. A comparison with the changes described in the articular cartilage of other species including man suggests that the phenomena observed are inherent age-related changes. The loss of activity of all the enzymes investigated indicates a far-reaching derangement of cellular metabolism, leading to impaired synthesis of proteoglycans. Since these compounds are major determinants of the physical properties of articular cartilage, the changes described might render the cartilage more vulnerable and might thus play a major part in the pathogenesis of osteoarthrosis.

show abstract

Section: Discussionsupporting

confidence: 77%

Age-Related Histochemical and Histological Changes in the Knee-Joint Cartilage of C57B1 Mice and Their Significance for the Pathogenesis of Osteoarthrosis

Pataki¹,

Rüttner²,

Abt³

1980

Pathobiology

View full text Add to dashboard Cite

show abstract

“…It is possible that in the mice used for the present experiments, functional stimulation may have been more marked than is suggested by the structural findings. However, there may be another reason for the comparatively low level response of these chondrocytes: the age of the mice used corresponds to middle rather than to old age; during this period of life, certain enzyme activities in articular cartilage of guinea pigs were lower than at all other ages [10], and this suggests that synthesis of structural cell products may also be at a minimum at this age. The deposition of increased amounts of glycogen in chondrocytes, which was not observed in young adult mice treated with the extract may be related to the absence of other synthetic activities.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of Articular Chondrocytes of Middle-Aged Mice by a Cartilage-Bone Marrow Extract

Silberberg¹,

Hasler²

1971

Pathobiology

View full text Add to dashboard Cite

Articular chondrocytes of 1-year-old male mice of strain C₅₇B1 were stimulated by injections of a cartilage-bone marrow extract to undergo hypertrophy, intensified organellar development and hyperplasia. This response was attenuated as compared with that of growing animals treated similarly. The morphological findings are consistent with some increase in synthetic activity on the part of the chondrocytes. The increased cell death observed was attributed to overstimulation and subsequent exhaustion, and was considered responsible for the prominence of microscars in the matrix.

show abstract

“…Solid lines represent the values obtained for the shoulder joints and shown in detail in Tables 3 and 5; broken lines represent the corresponding figures for the joints of the lower extremities, published previously in detail (Silberberg et al, 1970;Silberberg & Lesker, 1971;Silberberg, Lesker, & Hasler, in press). The four curves for each enzyme in males or females and upper or lower extremity respectively are drawn on the same scale; the scales for different enzymes are not always identical, since activities are variously expressed in moles, millomoles, micromoles, or grams.…”

Section: Histochemistrymentioning

confidence: 90%

“…(Silberberg et al, 1970, Silberberg & Lesker, 1971. Owing to the scarcity of animals in the oldest age group and to the small amount of cartilage available in old animals, not all enzymes could be examined in each animal.…”

Section: Methodsmentioning

confidence: 99%

Aging of the Shoulder Joint of Guinea Pigs. Electron Microscopic and Quantitative Histochemical Aspects

Silberberg¹,

Hasler²,

1973

Journal of Gerontology

Self Cite

View full text Add to dashboard Cite

Aging Changes in Ultrastructure and Enzymatic Activity of Articular Cartilage of Guinea Pigs

Cited by 34 publications

References 0 publications

Age-Related Histochemical and Histological Changes in the Knee-Joint Cartilage of C57B1 Mice and Their Significance for the Pathogenesis of Osteoarthrosis

Age-Related Histochemical and Histological Changes in the Knee-Joint Cartilage of C57B1 Mice and Their Significance for the Pathogenesis of Osteoarthrosis

Stimulation of Articular Chondrocytes of Middle-Aged Mice by a Cartilage-Bone Marrow Extract

Aging of the Shoulder Joint of Guinea Pigs. Electron Microscopic and Quantitative Histochemical Aspects

Contact Info

Product

Resources

About