Aggregation-induced emission of tetraphenylethene derivative as a fluorescence method for probing the assembling/disassembling of amphiphilic molecules

Zhu, Congshan; Pang, Shaopeng; Xu, Jun; Jia, Lan; Xu, Fangming; Mei, Ju; Qin, Anjun; Sun, Jingzhi; Ji, Jian; Tang, Benzhong

doi:10.1039/c1an15176b

Cited by 46 publications

(35 citation statements)

References 40 publications

(44 reference statements)

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“…Due to the strong dependence of the CMC on temperature, the concept of the CMT has been extensively used. 17 Temperature induces a crucial effect on the micellization process; thus, we decided to evaluate the CMT of PLGA at a concentration (0.48 g/L) that exceeded the CMC value of PLGA (0.31 g/L) obtained in the above-mentioned CMC experiment. The representative fluorescence spectra of the PLGA solution at a concentration of 0.48 g/L were recorded at different temperatures over the range 10–80 °C.…”

Section: Resultsmentioning

confidence: 99%

A Novel Study on the Self-Assembly Behavior of Poly(lactic-co-glycolic acid) Polymer Probed by Curcumin Fluorescence

2022

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Understanding the self-assembly behavior of block copolymers is of great importance due to their usefulness in a wide range of applications. In this work, the physical properties of poly(lactic- co -glycolic acid) (PLGA polymer) are studied for the first time in solution using the fluorescence technique and curcumin as a molecular probe. First, curcumin at a concentration of 2 μM was added to different concentrations of PLGA, and the fluorescence of curcumin was tracked. It was found that the critical micellar concentration (CMC) was equal to 0.31 g/L and the critical micellar temperature (CMT) was obtained to be 25 °C. Furthermore, an insight on the effect of NaCl salt on the CMC value of PLGA is assessed through curcumin probing. A decrease in the CMC has been observed with the increase in the concentration of NaCl, which could be due to the salting out effect. Moreover, in order to understand the aggregation behavior of PLGA in different solutions, CMC experiments were investigated using chloroform as a solvent. Results showed that the solvent does not affect the CMC value of the polymer; however, it only affects the shape of the obtained micelle forming a reversed micelle. Finally, fluorescence quenching of curcumin with hydrophobic cetyl-pyridinium bromide (CPB) and hydrophilic KI quenchers was established, where it was proved that curcumin is located near the hydrophobic pocket of the Stern layer of the PLGA micelle.

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Section: Resultsmentioning

confidence: 99%

A Novel Study on the Self-Assembly Behavior of Poly(lactic-co-glycolic acid) Polymer Probed by Curcumin Fluorescence

2022

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“…Meanwhile, the purity of CTGP was up to 90% without further purification by high‐performance liquid chromatography assay (Figure S2B, Supporting Information). Besides, the critical micelle formation concentration (CMC) of CTGP was estimated in water via fluorescence intensity analysis as reported . As shown in Figure S3 in the Supporting Information, the CMC of CTGP was 0.168 mg mL −1 , indicating that they could self‐assemble into nanomicelles at a concentration equal to or above the CMC.…”

Section: Methodsmentioning

confidence: 99%

A Transformable Chimeric Peptide for Cell Encapsulation to Overcome Multidrug Resistance

Zhang

Liu

Qiu

et al. 2018

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Multidrug resistance (MDR) remains one of the biggest obstacles in chemotherapy of tumor mainly due to P-glycoprotein (P-gp)-mediated drug efflux. Here, a transformable chimeric peptide is designed to target and self-assemble on cell membrane for encapsulating cells and overcoming tumor MDR. This chimeric peptide (C -K(TPE)-GGGH-GFLGK-PEG , denoted as CTGP) with cathepsin B-responsive and cell membrane-targeting abilities can self-assemble into nanomicelles and further encapsulate the therapeutic agent doxorubicin (termed as CTGP@DOX). After the cleavage of the Gly-Phe-Leu-Gly (GFLG) sequence by pericellular overexpressed cathepsin B, CTGP@DOX is dissociated and transformed from spherical nanoparticles to nanofibers due to the hydrophilic-hydrophobic conversion and hydrogen bonding interactions. Thus obtained nanofibers with cell membrane-targeting 16-carbon alkyl chains can adhere firmly to the cell membrane for cell encapsulation and restricting DOX efflux. In comparison to free DOX, 45-time higher drug retention and 49-fold greater anti-MDR ability of CTGP@DOX to drug-resistant MCF-7R cells are achieved. This novel strategy to encapsulate cells and reverse tumor MDR via morphology transformation would open a new avenue towards chemotherapy of tumor.

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“…It is well-established that in aqueous solutions of amphiphilic polymers, pyrene is solubilized into hydrophobic domains, showing a decrease in I 1 /I 3 . Thus, pyrene is a useful fluorescence probe for the characterization of molecular assemblies of associating polymers, and for the evaluation of the micropolarity of the local environment [37]. Figure 7a, b shows the change of I 1 /I 3 as a function of EAPPF or EAPO concentrations for EAPPF/TBCP and EAPPF/PEG mixed aqueous solutions, respectively.…”

Section: Micropolarity Of Micellementioning

confidence: 99%

Interaction Between an Ethoxylated Alkylphenol Polymer with Formaldehyde and Triblock polyEO–polyPO–polyEO Copolymer in Aqueous Solutions

Xin

Zhu

Cao

et al. 2013

J Surfact & Detergents

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The interaction between an ethoxylated alkylphenol polymer with formaldehyde (EAPPF) and a triblock polyEO-polyPO-polyEO copolymer (TBCP) in aqueous solutions has been investigated in detail by means of surface tension, steady-state fluorescence, dynamic light scattering (DLS) measurements and computer simulations. For comparison, the ethoxylated alkylphenol oligomer (EAPO), which is the monomer unit of EAPPF, and another nonionic water-soluble polymer PEG were also selected to get more information about the interaction between different types of surfactants and polymers. The surface properties of mixed systems at the air/water surface were evaluated from surface tension measurements. Information about the hydrophobic microenvironment and size of the aggregates was obtained from steady-state fluorescence using pyrene as a hydrophobic probe and DLS measurements, respectively. The dissipative particle dynamics simulation method was applied to simulate the interaction between EAPPF (or EAPO) and TBCP (or PEG) in aqueous solutions. The synergetic interaction between EAPPF (or EAPO) and TBCP in binary mixed solution enhanced the adsorption of surfactant molecules (EAPPF or EAPO) at the interface, while no obvious interaction between EAPPF (or EAPO) and PEG was observed.

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Aggregation-induced emission of tetraphenylethene derivative as a fluorescence method for probing the assembling/disassembling of amphiphilic molecules

Cited by 46 publications

References 40 publications

A Novel Study on the Self-Assembly Behavior of Poly(lactic-co-glycolic acid) Polymer Probed by Curcumin Fluorescence

A Novel Study on the Self-Assembly Behavior of Poly(lactic-co-glycolic acid) Polymer Probed by Curcumin Fluorescence

A Transformable Chimeric Peptide for Cell Encapsulation to Overcome Multidrug Resistance

Interaction Between an Ethoxylated Alkylphenol Polymer with Formaldehyde and Triblock polyEO–polyPO–polyEO Copolymer in Aqueous Solutions

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