Aggregation Characteristics of Laser Dye Rhodamine 6g in Aqueous Surfactant Solutions

Kelkar, V. K.; Valaulikar, B. S.; Kunjappu, Joy T.; Manohar, C.

doi:10.1111/j.1751-1097.1990.tb08672.x

Cited by 32 publications

(24 citation statements)

References 12 publications

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“…Increasing the extracellular R110 concentration to 25 M also leads to a plateau, but its level is not proportional to the extracellular concentration used. This presumably can be attributed to the lower quantum yields of R110 aggregates (Deumié and El Baraka 1993;Kelkar et al 1990;Bunting et al 1989) and indicates that, as for R123 (Canitrot 1994), there is no binding of R110 to a limited number of identical binding sites. In such a case we should have obtained the same plateau level regardless of the extracellular R110 concentration.…”

Section: Discussionmentioning

confidence: 99%

Intracellular Accumulation of Rhodamine 110 in Single Living Cells

Jeannot

Salmon

Deumié

et al. 1997

J Histochem Cytochem.

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SUMMARYTo gain a better understanding of the internalization of rhodamines, vital staining of living cells in situ by two different rhodamines, R110 and R123, was studied by microfluorometry. These dyes differ strongly in their lipophilic properties because of differences in charge distribution. Microspectrofluorometry was used to study the fluorescence emission spectra of R110-loaded cells to determine reliable loading conditions. Cell uptake and cell efflux studies of R110 were performed by numerical microfluorescence imaging. A slower uptake was observed for R110 (14 hr) vs R123 (2 hr), but the R110 efflux was much more rapid (30 min) than that of R123 ( Ͼ 24 hr). Although it appeared in the R110 and R123 co-localization study that R110 was able to accumulate in mitochondria, labeling with R110 was lower than with R123. Our results indicate that, rhodamine 110 in its acid cationic form is able to cross the plasma and mitochondrial membrane and to accumulate in cell compartments as does the cationic rhodamine 123. However, because of its acido-basic properties, R110 should be able to decrease the pH of cell compartments, depending on their ability to regulate pH. In such a model, mitochondrial pH should be more greatly decreased than cytosolic pH, leading to a lower mitochondrial accumulation of R110 than of R123. Surprisingly, these effects, which should affect the energetic state of mitochondria, do not influence cell growth, because no cytotoxic effect was observed.( J Histochem Cytochem 45:403-412, 1997 )

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Section: Discussionmentioning

confidence: 99%

Intracellular Accumulation of Rhodamine 110 in Single Living Cells

Jeannot

Salmon

Deumié

et al. 1997

J Histochem Cytochem.

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“…The formation of dye-surfactant aggregates as well as the distribution of dye between aqueous and micellar phase have been some times described by the association equilibria [16,20,[40][41][42][43][44] where iD þ jS À À! À À D i S j , illustrates the formation of mixed clusters between dye (D) molecules and monomeric surfactant (S) and yD þ S n À À!…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic analysis of naphtholazobenzimidazole in organised solution

Shah

Naeem

Naseem

et al. 2010

Physics and Chemistry of Liquids

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“…19,20 These probes are based on the formation of non-fluorescent dye dimers. The phenomenon of aggregation and dimerization of fluorescent dyes in aqueous solutions has been shown for a wide variety of fluorescent dyes, e. g. the xanthene 21,22 , phenothiazine 23 , squarine 24 , BODIPY 25,26 and cyanine 27,28 dyes. Usually, these dyes form Htype dimers (face-to-face conformation) in aqueous solutions.…”

Section: Dimer Probesmentioning

confidence: 99%

New hairpin-structured DNA probes: alternatives to classical molecular beacons

et al. 2007

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In this article we report on two different classes of self-quenching hairpin-structured DNA probes that can be used as alternatives to Molecular Beacons. Compared to other hairpin-structured DNA probes, the so-called smart probes are labeled with only one extrinsic dye. The fluorescence of this dye is efficiently quenched by intrinsic guanine bases via a photo-induced electron transfer reaction in the closed hairpin. After hybridization to a target DNA, the distance between dye and the guanines is enlarged and the fluorescence is restored. The working mechanism of the second class of hairpin DNA probes is similar, but the probe oligonucleotide is labeled at both ends with an identical chromophore and thus the fluorescence of the closed hairpin is reduced due to formation of non-fluorescent dye dimers. Both types of probes are appropriate for the identification of single nucleotide polymorphisms and in combination with confocal single-molecule spectroscopy sensitivities in the picomolar range can be achieved.

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Aggregation Characteristics of Laser Dye Rhodamine 6g in Aqueous Surfactant Solutions

Cited by 32 publications

References 12 publications

Intracellular Accumulation of Rhodamine 110 in Single Living Cells

Intracellular Accumulation of Rhodamine 110 in Single Living Cells

Spectroscopic analysis of naphtholazobenzimidazole in organised solution

New hairpin-structured DNA probes: alternatives to classical molecular beacons

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