In order to illustrate the binding of a small molecule to a protein, the authors pose the described florescent assay to students of a chemistry and biology section.
The CC14 quenching of the monomeric (M) and excimer (D) fluorescence of poly(2-vinylnaphthalene) (P2VN) and poly(lV-vinylcarbazole) (PVCz) in room-temperature CH2C12 solutions is compared with monomeric and dimeric model compounds in order to estimate As, the singlet migration rate. Values in excess of 10~5 cm2 s"1 were found for the M excited state of PVCz but Aa = 0 is estimated for the P2VN M and D states and the PVCz D state. In addition it was found that the concentration dependence for D quenching deviates from that expected from a model in which the M state is the only precursor to D. A kinetic model is proposed to account for these and other observations concerning the singlet excited state of polymers.
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