An investigation was made of the time course of perceptual grouping that is based on two qualitatively different spatial relationships: proximity and alignment. An index of grouping capacity was used to assess the processing time required before a backward pattern mask interfered with grouping. Stimuli consisted of bistable arrays of disjunct dots that were followed by a mask. Grouping cues, either proximity or alignment, were randomly assigned to either the horizontal or vertical orientation, and subjects indicated whether the dots appeared grouped as a series of horizontal or vertical lines. Spatial metrics of the cues were systematically altered until they no longer served as a cue for grouping, thereby determining the grouping threshold. The stimulus onset asynchrony (SOA)of the mask, relative to the test stimulus, ranged from 33.3 to 150 msec. The SOAat which grouping thresholds first became elevated identified the point at which the mask first interfered with the grouping process, thereby identifying the processing time required for grouping by the specified cue. The processing time for grouping by proximity and alignment differed significantly, requiring means of 87.6and 118.8msec, respectively, for processing to be completed. These measurements serve to identify the processing time necessary for spatially integrating stimulus elements into unified forms, thereby delineating temporal constraints at this stage of visual processing.In order to organize complex stimuli in preparation for object recognition, the visual system quickly and automatically associates and segregates elements of the visual scene. To accommodate novel stimuli with speed and accuracy, the process ofgrouping likely follows established algorithms that mediate grouping strategies. These algorithms operate by extracting relevant information from the stimuli and then applying a set ofcriteria to that information which specify the grouping arrangement best suited for form identification. Although many of the characteristics associated with perceptual grouping have been described, the principles by which grouping functions, and the time course of its operation, are not well understood.Grouping strategies are based on several characteristics of stimuli, including feature similarities, such as color or element shape, and spatial relationships, such as element proximity or regularity. The present study examined grouping that is based on two spatial relationships among stimulus elements: element proximity and element alignment. In each case, a single spatial parameter was systematically altered while observers made judgments about the manner in which elements appeared to be grouped. In this way, the association between a specific spatial metric and the process of grouping was isolated.Grouping by element proximity and element alignment differ qualitatively in the nature of the spatial cue from which grouping is derived. Grouping by proximity (Rush, 1937) is based on the tendency for more proximal images to produce unified configurations, and for...