Aggregation and denaturation of antibodies: a capillary electrophoresis, dynamic light scattering, and aqueous two-phase partitioning study

Bermudez, Ondrea; Forciniti, Daniel

doi:10.1016/j.jchromb.2004.01.029

Cited by 53 publications

(31 citation statements)

References 29 publications

(34 reference statements)

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“…11B), which had a hydrodynamic size (5.3 nM) comparable to previously reported values for other IgG molecules. [39][40][41][42][43] Homology modeling suggested that the succinimide intermediate is stabilized via π interaction with the side chain of an adjacent tryptophan residue. Using SPR analysis, we found a moderate decrease in binding affinity of mAb-1, which contained one succinimide105 modification on the HC, compared with a fraction containing no modifications (HC Asn105).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the in vitro and in vivo stability of a succinimide intermediate observed on a therapeutic IgG1 molecule

Ouellette

Chumsae²,

Clabbers³

et al. 2013

mAbs

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Section: Discussionmentioning

confidence: 99%

Comparison of the in vitro and in vivo stability of a succinimide intermediate observed on a therapeutic IgG1 molecule

Ouellette

Chumsae²,

Clabbers³

et al. 2013

mAbs

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“…Monitoring of physical changes is represented by the application of CZE to investigate the aggregation process of b-amyloid peptides [273], and aggregation and denaturation of antibodies [274]. CZE separation of folded and unfolded forms of polypeptides and proteins allows studying the equilibria and kinetics of conformation transition states during polypeptide and protein folding/ unfolding/refolding processes [275].…”

Section: Monitoring Of Chemical and Enzymatical Reactions And Physicamentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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“…CZE was used to study b-amyloid and its aggregation [275], botulinum neurotoxin B light chain activity [276], nonenzymatic posttranslation modifications on BSA [277], aminoacyl-tRNA synthetase localized in cell nucleus [278], protein-protein interactions [279], aggregation and denaturation of antibodies [280], glycosylation of murine erythropoietin [281], PEGylation of proteins [282], glutamine deamidation in proteins [283], complexation of apomyoglobin with Coumarin 153 [284], reaction of FQ with ovalbumin [285], interaction of GFP with calcium [286], noncovalent complexation in myoglobin [287], DNA-protein interactions [288][289][290], binding fucoidan to antithrombin [291], retinoic acid to b-lactoglobulin B [292], and binding phospholipid and glycosaminoglycan to human b 2 -glycoprotein [293].…”

Section: Other Proteinsmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Aggregation and denaturation of antibodies: a capillary electrophoresis, dynamic light scattering, and aqueous two-phase partitioning study

Cited by 53 publications

References 29 publications

Comparison of the in vitro and in vivo stability of a succinimide intermediate observed on a therapeutic IgG1 molecule

Comparison of the in vitro and in vivo stability of a succinimide intermediate observed on a therapeutic IgG1 molecule

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Capillary electrophoresis of proteins 2003–2005

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