Aggregate-formation by Clostridium butyricum

Zoutberg, George R.; Willemsberg, R.; Smit, G.; Mattos, M. Joost Teixeira de; Neijssel, Oense M.

doi:10.1007/bf00164816

Cited by 18 publications

(15 citation statements)

References 17 publications

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“…The latter negative effect may be caused by the uncoupling effects of fatty acids (12). Especially fatty acids with medium chain length would be potent in this respect (12,37). It is tempting to speculate that such toxicity has even provided the selection pressure for the evolution of the fatty acid-dependent repression mechanism of lipA expression.…”

Section: Discussionmentioning

confidence: 99%

Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA

Kok¹,

Nudel²,

Gonzalez³

et al. 1996

J Bacteriol

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The extracellular lipase (LipA) produced by Acinetobacter calcoaceticus BD413 is required for growth of the organism on triolein, since mutant strains that lack an active lipase fail to grow with triolein as the sole carbon source. Surprisingly, extracellular lipase activity and expression of the structural lipase gene (lipA), the latter measured through lacZ as a transcriptional reporter, are extremely low in triolein cultures of LipA ؉ strains. The explanation for this interesting paradox lies in the effect of fatty acids on the expression of lipA. We found that long-chain fatty acids, especially, strongly repress the expression of lipA, thereby negatively influencing the production of lipase. We propose the involvement of a fatty acyl-responsive DNA-binding protein in regulation of expression of the A. calcoaceticus lipBA operon. The potential biological significance of the observed physiological competition between expression and repression of lipA in the triolein medium is discussed. Activity of the extracellular lipase is also negatively affected by proteolytic degradation, as shown in in vitro stability experiments and by Western blotting (immunoblotting) of concentrated supernatants of stationaryphase cultures. In fact, the relatively high levels of extracellular lipase produced in the early stationary phase in media which contain hexadecane are due only to enhanced stability of the extracellular enzyme under those conditions. The rapid extracellular degradation of LipA of A. calcoaceticus BD413 by an endogenous protease is remarkable and suggests that proteolytic degradation of the enzyme is another important factor in regulating the level of active extracellular lipase.

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Section: Discussionmentioning

confidence: 99%

Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA

Kok¹,

Nudel²,

Gonzalez³

et al. 1996

J Bacteriol

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“…The continuous-culture medium was similar, except that 0.13 g/L CaCl 2 , 0.0535 g/L (1.0 mM) NH 4 Cl, and 0.30 g/L (10 mM-C) glucose were used. This medium was low in nitrogen (C/N ratio 10:1) to stimulate exopolymer production (Huang et al, 1994;Straight and Ramkrishna, 1994) and contained extra calcium to improve biofilm strength (Marshall et al, 1971;Turakhia and Characklis, 1989;Zoutberg, 1990). For routinely checking of culture purity plate count agar (5 g/L tryptone, 2.5 g/L yeast extract, 1 g/L glucose, 18 g/L agar, pH 7.0) was used.…”

Section: Bacterial Cultivationmentioning

confidence: 99%

Adhesion and biofilm development on suspended carriers in airlift reactors: Hydrodynamic conditions versus surface characteristics

Gjaltema

Marel

Loosdrecht

et al. 1997

Biotechnol. Bioeng.

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“…Aggregate formation by Clostridia (Zoutberg et al, 1989) has been utilized in recently developed carrier induced granular sludge bed reactors (CIGSBs) and has resulted in efficient biomass retention and high H 2 production rates up to 7.66 L h À1 L À1 from sucrose (Lee et al, 2004(Lee et al, , 2006. CIGSBs may be more stable for H 2 fermentation than conventional biofilm reactors; biofilms may favour propionate-type fermentation, whereas granulation favours H 2 producing Clostridia.…”

mentioning

confidence: 99%

The relationship between instability of H₂ production and compositions of bacterial communities within a dark fermentation fluidised‐bed bioreactor

Koskinen

Kaksonen

Puhakka

2007

Biotech & Bioengineering

125

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Microbial community composition dynamics was studied during H(2) fermentation from glucose in a fluidized-bed bioreactor (FBR) aiming at obtaining insight into the H(2) fermentation microbiology and factors resulting in the instability of biofilm processes. FBR H(2) production performance was characterised by an instable pattern of prompt onset of H(2) production followed by rapid decrease. Gradual enrichment of organisms increased the diversity of FBR attached and suspended-growth phase bacterial communities during the operation. FBR bacteria included potential H(2) producers, H(2) consumers and neither H(2) producers nor consumers, and those distantly related to any known organisms. The prompt onset of H(2) production was due to rapid growth of Clostridium butyricum (99-100%) affiliated strains after starting continuous feed. The proportion trend of C. butyricum in FBR attached and suspended-growth phase communities coincided with H(2) and butyrate production. High glucose loading rate favoured the H(2) production by Escherichia coli (100%) affiliated strain. Decrease in H(2) production, associated with a shift from acetate-butyrate to acetate-propionate production, was due to changes in FBR attached and suspended-growth phase bacterial community compositions. During the shift, organisms, including potential propionate producers, were enriched in the communities while the proportion trend of C. butyricum decreased. We suggest that the instability of H(2) fermentation in biofilm reactors is due to enrichment and efficient adhesion of H(2) consumers on the carrier and, therefore, biofilm reactors may not favour mesophilic H(2) fermentation.

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Aggregate-formation by Clostridium butyricum

Cited by 18 publications

References 17 publications

Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA

Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA

Adhesion and biofilm development on suspended carriers in airlift reactors: Hydrodynamic conditions versus surface characteristics

The relationship between instability of H₂ production and compositions of bacterial communities within a dark fermentation fluidised‐bed bioreactor

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Aggregate-formation by Clostridium butyricum

Cited by 18 publications

References 17 publications

Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA

Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA

Adhesion and biofilm development on suspended carriers in airlift reactors: Hydrodynamic conditions versus surface characteristics

The relationship between instability of H2 production and compositions of bacterial communities within a dark fermentation fluidised‐bed bioreactor

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The relationship between instability of H₂ production and compositions of bacterial communities within a dark fermentation fluidised‐bed bioreactor