Agglutinationsverhalten von Hefen mit Lektinen

Meinhold, I.; Gabert, A; Wünsche, L.; Roth, P.

doi:10.1002/jobm.3630191009

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…The role of lectins in microbiological diagnosis has been reviewed in detail by several authors 30,31,32,33 . In the case of yeasts, lectins bind to α‐mannans, which are abundant on the cell surface; 19,21,22,34 studies with C. albicans and ConA indicate that lectin binding is abolished when these sugars are removed by chemical treatment 20,35 …”

Section: Discussionmentioning

confidence: 99%

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Lectin typing of five medically important Candida species

Muñoz¹,

Alonso²,

Álvarez³

et al. 2003

Mycoses

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This study reports the patterns of agglutination of 93 clinical Candida isolates by 14 commercial lectins. The isolates were of the species Candida albicans (55), C. tropicalis (12), C. guilliermondii (10). C. glabrata (eight) and C. parapsilosis (eight). Hundred percent of isolates were agglutinated, at least, by a panel of three lectins: Canavalia ensiformis (ConA), Lens culinaris (LCA) and Pisum sativum (PSA), all of them with alpha-D-mannose specificity. In addition, another panel of three lectins could distinguish between C. glabrata, C. tropicalis and C. albicans. Lectin typing may be of potential value for taxonomic and epidemiological studies of yeasts in clinical laboratories.

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Section: Discussionmentioning

confidence: 99%

“…Meinhold et al 34 . studied the agglutination of 24 Candida isolates (22 C. guilliermondii , one C. tropicalis , one C. parapsilosis ) by six lectins: ConA agglutinated all isolates and LCA all except one C. guilliermondii isolate and one C. tropicalis isolate, while WGA and DBA did not agglutinate any isolates.…”

Section: Discussionmentioning

confidence: 99%

Lectin typing of five medically important Candida species

Muñoz¹,

Alonso²,

Álvarez³

et al. 2003

Mycoses

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Wechselwirkungen zwischen Lektinen und Mikroorganismen I. Bestimmung der Agglutinationsgeschwindigkeit durch Extinktionsmessungen: Agglutination von Hefezellen (Saccharomyces cerevisiae H155) durch Concanavalin A

Flemming¹,

Gabert²,

Flemming³

1985

J. Basic Microbiol.

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A simple photometric method for the determination of the agglutination rate of cells by lectins in a continuously stirred suspension is presented. Besides the agglutination rate the method allows the estimation of an average degree of agglutination, i.e. the number of cells per aggregate. The influence of the Con A-concentration, cell number, temperature, and pH on the agglutination rate of Saccharomyces cerevisiae H155 have been studied. The results are of good reproducibility and, therefore, the methods is suitable to describe interactions between cell-bound receptors and receptor-specific proteins, e.g. lectins and antibodies.

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The Kinetics of Lectin-Mediated Cell Agglutination

Flemming¹

1991

Advances in Lectin Research

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Agglutinationsverhalten von Hefen mit Lektinen

Cited by 5 publications

References 7 publications

Lectin typing of five medically important Candida species

Lectin typing of five medically important Candida species

Wechselwirkungen zwischen Lektinen und Mikroorganismen I. Bestimmung der Agglutinationsgeschwindigkeit durch Extinktionsmessungen: Agglutination von Hefezellen (Saccharomyces cerevisiae H155) durch Concanavalin A

The Kinetics of Lectin-Mediated Cell Agglutination

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