2021
DOI: 10.1002/elsc.202000112
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Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification

Abstract: Purification of mRNA with oligo(dT)‐functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can result in a decreased total surface area and thus a decreased yield of mRNA. In addition, agglomeration may also be caused by mRNA loading on the magnetic particles. Therefore, it is of interest how the individual steps of magnetic separation and subsequent redisper… Show more

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Cited by 3 publications
(4 citation statements)
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“…Due to the relatively open structure of the separator matrix for allowing optical measurements, magnetic induced agglomeration within the separation chamber is beneficial. As described in literature [11,31], magnetic beads attract each other when a magnetic field is applied. This agglomeration behavior is amplified with higher concentrations due to higher contact possibilities of single particles.…”
Section: Separation Experimentsmentioning
confidence: 97%
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“…Due to the relatively open structure of the separator matrix for allowing optical measurements, magnetic induced agglomeration within the separation chamber is beneficial. As described in literature [11,31], magnetic beads attract each other when a magnetic field is applied. This agglomeration behavior is amplified with higher concentrations due to higher contact possibilities of single particles.…”
Section: Separation Experimentsmentioning
confidence: 97%
“…For performing separation experiments, a special batch of M-PVA-C22 magnetic beads (PerkinElmer chemagen Technologie GmbH, Germany) in deionized water was chosen. With a carboxylic group the magnetic beads can be functionalized with enzymes [2,14] or oligonucleotides, making them suitable for mRNA purification [11,28]. Magnetic bead concentrations of c B,0 = 0.2-4 g L -1 were used for performing HGMS.…”
Section: High-gradient Magnetic Separationmentioning
confidence: 99%
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“…Due to the high surface-to-volume ratio and the control of surface properties, magnetic particles are very suitable for cell capture [5]. In addition, the dynamic behavior of magnetic particles can be precisely controlled using an external magnetic field, such as performing buffer exchange washing step [6], rapid immunoassay [7,8], or achieving effective fluid mixing [9][10][11][12]. With the increasing demand for the design of artificial external control structures, the precise control of magnetic particles under the magnetic field has become very important.…”
Section: Introductionmentioning
confidence: 99%