Agentes osmóticos e temperatura na conservação in vitro de sempre-viva

Brito, Alone Lima; Albuquerque, Mara Márcia Sampaio; Alvim, Bruno Freitas Matos; Resende, Sheila Vitória; Bellintani, Moema Cortizo; Santana, José Raniere Ferreira de

doi:10.1590/s0103-84782011000800010

Cited by 14 publications

(9 citation statements)

References 19 publications

(19 reference statements)

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“…The findings of the current study, namely that the highest number of green leaves was obtained in media supplemented with sucrose (15 and 30 g L -1 ), are in accordance with those of Lima-Brito et al (2011), who analyzed the effect of osmotic agents and temperature in the in vitro conservation of Syngonanthus mucugensis Giul. subsp.…”

Section: Resultssupporting

confidence: 89%

Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims

Souza¹,

Silva²,

Ciano³

et al. 2021

J. Med. Plants Res.

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Passiflora edulis Sims, besides having economic and social importance, is included in the medicinal plant list that is considered of interest by the Unified Health System (Sistema Único de Saúde -SUS). Due to its high nutritional value, this species is widely used in food, cosmetics and pharmaceutical industries. It also contains carotenoids, as well as passiflorine and maracugine, which have sedative effects. The aim of this research was to assess P. edulis minimum growth conditions in different concentrations and combinations of MS culture medium and sucrose for in vitro conservation. The following variables were analyzed: Plant height; number of green and senescent leaves; and number of roots. Root height means were highest in MS media containing 30 g L -1 sucrose (8.33). With regard to plant height, the best results were obtained in MS/4 media with 15 g L -1 or 30 g L -1 sucrose, which enabled the maintenance of lower plants (5.35 and 4.91, respectively) with green leaves after 120 days in vitro culture. The MS/2 media supplemented with 15 g L -1 sucrose was the best alternative for in vitro maintenance of plants with appropriate height and number of green leaves (8.22 and 3.86, respectively). Thus, we may conclude that MS/2 with 15 g L -1 sucrose represents an efficient strategy for in vitro conservation of P. edulis plants for a culture period of 120 days and 100% survival after acclimatization.

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Section: Resultssupporting

confidence: 89%

Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims

Souza¹,

Silva²,

Ciano³

et al. 2021

J. Med. Plants Res.

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“…For C. mucugensis, the presence of mannitol led to the lowest means for SL in the study conducted by Lima-Brito et al (2011) when compared to the presence of other carbohydrates such as sorbitol and sucrose.…”

Section: Resultsmentioning

confidence: 77%

“…Parra & Giul. (Erirocaulaceae) (LIMA-BRITO et al, 2011) and for Vanilla planifolia Jacks. ex Andrews (Orquidaceae) (BELLO-BELLO; GARCÍA-GARCÍA; IGLESIAS-ANDREU, 2015) for a period of 180 days.…”

Section: Resultsmentioning

confidence: 99%

MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE)1

et al. 2021

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Bromeliads are the target of predatory extractivism and consequently many species are included in the red list of threatened species, such as those belonging to the genus Neoregelia. Although Neoregelia mucugensis has not been evaluated for the degree of threat, its exploitation is exclusively extractive and its occurrence in Chapada Diamantina-BA is subject to the action of fires that affect the region annually. In this context, applying techniques aimed at protecting this genetic resource is fundamental for both the maintenance of its natural populations and the ex situ conservation of this genetic material. Plant tissue culture techniques have been successfully applied for the conservation of several bromeliad species. One of the methods used is slow growth, which consists in reducing plant metabolism and consequently decelerating its growth, which allows the maintenance of in vitro plant collections without the need for subculture. In this context, the objective of this study was to test the reduction of salts in the culture medium and the addition of osmoregulators on the induction of slow growth of N. mucugensis. Plants were subjected to treatments composed of different concentrations of MS medium and mannitol for a period of 12 months, when then analyses were conducted to evaluate growth, chlorophyll content and regeneration capacity of shoots in vitro. It was found that the treatment containing MS ½ and 7.8 g.L-1 of mannitol is indicated for in vitro conservation of N. mucugensis with maintenance of the regenerative capacity of its tissues.

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“…The %S observed in the present study were higher than those found for other species submitted to slow growth storage with osmotic agents. For example, Syngonanthus mucugensis species from Chapada Diamantina, cultivated in 45 and 60 g L − 1 of sucrose for 180 days, showed low survival (< 35%) (Lima-Brito et al 2011). Although, Silva et al (2019) observed for Poincianella pyramidalis, typical of drought environment, values above 67% for 240 days cultivated in 30, 45, 60 and 75 g L − 1 of sucrose.…”

Section: Discussionmentioning

confidence: 99%

In vitro conservation and genetic diversity of threatened species of Melocactus (Cactaceae)

et al. 2021

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The high endemism, the natural habitat degradation, and the over-collection for ornamental purposes have led some species such as Melocactus paucispinus and M. glaucescens to be threatened with extinction. The use of in vitro conservation techniques, such as slow growth storage, promotes the preservation of genetic diversity with integrity. The goal of this study was to establish a strategy for in vitro conservation of apical segments of the cladode of M. paucispinus and M. glaucescens and evaluate the genetic diversity of individuals from in vitro germinated plants. For such purpose, different concentrations of the plant regulator ancymidol and the osmotic agent sucrose on the inhibition of the in vitro growth were tested, and the genetic diversity of M. paucispinus and M. glaucescens individuals stored in vitro was evaluated. Sucrose showed higher e ciency in the reduction of growth than ancymidol for both species. However, due to the reduction in survival percentage, the use of sucrose over 75 g L − 1 in the in vitro conservation of both species for 360 days is not recommended. In the genetic diversity analysis, 76.92% of polymorphic loci (P), expected heterozygosity (He) = 0.276 and Shannon index (S) = 0.414 were observed for M. paucispinus. For M. glaucescens, the observed values were P = 95.38%, He = 0.228 and S = 0.369. These values observed here were higher than those previously found for the natural populations of these species, which demonstrated that this in vitro collection showed genetic diversity and can be used in management and reintroduction programs of these species.

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Agentes osmóticos e temperatura na conservação in vitro de sempre-viva

Abstract: IVAgentes osmóticos e temperatura na conservação in vitro de sempre-viva Osmotic agents and temperature on in vitro conservation of sempre-viva

Cited by 14 publications

References 19 publications

Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims

Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims

MODULATION OF CULTURE MEDIUM ON THE EX SITU CONSERVATION OF Neoregelia mucugensis Leme (BROMELIACEAE)1

In vitro conservation and genetic diversity of threatened species of Melocactus (Cactaceae)

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