Ageing-related changes in the levels of β-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7 in the heart of men

Abstract

Aging is a major risk factor for morbidity and mortality from cardiovascular causes in men. To better understand the cellular processes related to age-related cardiac complications, we undertook research aimed at comparative evaluation of genes expression and distribution of β-catenin, CacyBP/SIP, galectin-3 and LMP7 in the heart of healthy men in different age groups. The study was conducted on the hearts of 12 men (organ donors) without a history of cardiovascular disease, who were divided into two age groups: men under and men over 45 years of age. On paraffin sections, immunohistochemical reactions were performed to detect β-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7. The expression of genes coding β-catenin, CacyBP/SIP, galectin-3 and LMP7 was also evaluated by real-time PCR method. In the heart of men over 45 years old, both gene expression and immunoreactivity of β-catenin, CacyBP/SIP, galectin-3 and LMP7 were stronger compared to younger individuals. The results of the presented studies suggest that β-catenin, CacyBP/SIP, galectin-3 and immunoproteasomes might be involved in the internal regulation of heart homeostasis during ageing.

Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model

“…The intensity of immunoreaction (brown color or gray, depending on staining) was measured by the quantitative comparison of mean pixel intensity values in the photomicrographs converted into 8-bit grayscale images. The scale was from 0 (white pixel) to 255 (black pixel); the lower the pixel value, the higher the intensity of immunohistochemical reaction [ 27 ]. The intensity of the immunoreaction in each of the analyzed digital images was measured in 10 randomly selected areas of the positive signal.…”

Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis

“…Negative control sections for each antibody were conducted by identical immunohistochemical staining excluding the primary antibody ( Supplementary Data, Figure S1 ). The intensity of immunoreaction was measured using a CX43 microscope (Olympus, Tokyo, Japan), both by determining the percentage of cells with a positive response, and by the quantitative comparison of mean pixel intensity in the photomicrographs, which were firstly converted into negatives and then into 8-bit grey-scale digital images, with a scale from 0 (white pixel) to 255 (black pixel), where the higher the pixel value, the higher the intensity of the immunohistochemical reaction [ 29 , 44 ]. For the immunoreactive cell count procedure, four randomly selected areas of compact bone, trabecular bone and articular cartilage, respectively, were measured for each microscopic slide.…”

Zapalenie starcze - mechanizmy i szlaki sygnałowe

“…Immunoproteasomy zaangażowane są głównie w tworzenie antygenów peptydowych prezentowanych przez cząsteczki głównego układu zgodności tkankowej klasy I (MHC klasy I). Ponadto są zaangażowane w degradację utlenionych białek [54][55][56]. W swoich badaniach Pickering i wsp.…”

“…Obserwowano ponadto zależne od wieku zmiany w podjednostkach tworzących proteasom w kierunku zwiększonej ekspresji immunopodjednostek [54]. W badaniach prowadzonych na ludzkim mięśniu sercowym pobranym post mortem od mężczyzn w grupach wiekowych <45 lat oraz 45-65 lat, obserwowano zwiększoną ekspresję mRNA i białka podjednostki LMP7 immunoproteasomu [56]. Wnioskuje się, że w te zależne od wieku zmiany mogą być zaangażowane mechanizmy kompensacji, mające na celu utrzymanie aktywności proteasomu oraz usuwanie utlenionych białek akumulujących się wraz z wiekiem [54,55].…”