Ageing of the human corneal stroma: structural and biochemical changes

Malik, Nageena S.; Moss, S.J.; Ahmed, Nessar; Furth, Anna J.; Wall, Rita S.; Meek, Keith Michael Andrew

doi:10.1016/0925-4439(92)90041-k

Cited by 245 publications

(158 citation statements)

References 15 publications

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“…Over a 90-year time span, there is, on average, an increase in cross-sectional area from approximately 3.04 to 3.46 nm 2 . 57 In contrast, there is a decrease in the interfibrillary distance with age, in accordance with Kanai and Kaufmann, 54 who also observed a greater breakdown of fibers, along with multiple small collagen-free spaces.…”

Section: Central Cornea During Developmentsupporting

confidence: 84%

Birefringence of the central cornea in children assessed with scanning laser polarimetry

Irsch

Shah

2012

J. Biomed. Opt

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Abstract. Corneal birefringence is a well-known confounding factor with all polarization-sensitive technology used for retinal scanning and other intraocular assessment. It has been studied extensively in adults, but little is known regarding age-related differences. Specifically, no information is available concerning corneal birefringence in children. For applications that are geared towards children, such as retinal birefringence scanning for strabismus screening purposes, it is important to know the expected range of both corneal retardance and azimuth in pediatric populations. This study investigated central corneal birefringence in children (ages three and above), by means of scanning laser polarimetry (GDx-VCC™, Carl Zeiss Meditec, Inc.). Children's measures of corneal retardance and azimuth were compared with those obtained in adults. As with previous studies in adults, corneal birefringence was found to vary widely in children, with corneal retardance ranging from 10 to 77 nm, and azimuth (slow axis) ranging from −11°to 71°(measured nasally downward). No significant differences in central corneal birefringence were found between children and adults, nor were significant age-related differences found in general. In conclusion, establishing knowledge of the polarization properties of the central cornea in children allows better understanding, exploitation, or bypassing of these effects in new polarization-sensitive pediatric ophthalmic applications.

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Section: Central Cornea During Developmentsupporting

confidence: 84%

Birefringence of the central cornea in children assessed with scanning laser polarimetry

Irsch

Shah

2012

J. Biomed. Opt

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“…[21][22][23][24] The changes in structural corneal biomechanics with subject age adds further complexity to the relationship between CCT and Goldmann IOP. 26 The diabetic patients in our study had significantly greater CCTs than the non-diabetic patients, although only by a mean of 9 mm. Although the eyes of diabetics (all type 2) had higher IOPs (a mean of 0.4 mmHg higher) in our study, this did not reach statistical significance in contrast to the results of larger comparative studies that included types 1 and 2 diabetics.…”

Section: Discussionmentioning

confidence: 44%

The relationship between central corneal thickness and the optic disc in an elderly population: the Bridlington Eye Assessment Project

Hawker

Edmunds

Vernon

et al. 2007

Eye

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Purpose To determine population-based normative CCT data for elderly white subjects, with and without diabetes, and to explore the relationship between CCT and IOP and HRT2 measurements in such subjects with normal visual fields. Methods All eligible subjects were consecutive in a population screened for eye disease. CCT was measured by ultrasound pachymetry, and the optic disc was morphometrically defined using Heidelberg retinal tomography (HRT2). Inclusion criteria were: (1) normal visual field on suprathreshold testing and (2) corrected logMAR acuity of at least 0.3 in both eyes. Subjects with significant corneal pathology, previous corneal surgery, or known history of glaucoma or treatment for raised intraocular pressure were excluded. One eye was randomly selected from each subject for analysis. Results In all, 983 eyes of 983 subjects were included with 690 HRT images deemed acceptable for the analysis. The mean age (414 men and 569 women) was 73.3 years (minimum 65 years). Mean (SD) CCT was 544.1 (36.5) lm, with a normally distributed range of 429-633 lm. There was no significant difference in CCT between men and women (mean CCT 546.1 and 542.7 lm, respectively, P ¼ 0.15, Student's t-test), though CCT was correlated weakly and negatively with age (Pearson's r ¼ À0.063, P ¼ 0.047). Diabetic patients (n ¼ 103) had a greater mean CCT than non-diabetic patients (551.9 and 543.0 lm respectively, P ¼ 0.02). No significant correlation was found between any global optic disc parameter and CCT in the 690 eyes analysed. Conclusion Elderly white eyes with normal fields have CCTs that are normally distributed, with those from diabetic persons having greater CCTs. No clear evidence of a relationship between CCT and HRT2 optic disc parameters used in glaucoma diagnosis was found.

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“…A perfectly regular structure of corneal stroma is formed from fine lamellae composed of narrow, uniform diameter collagen fibrils and the lamellae are separated by layers of hydrogel composed of small proteoglycan [37,51]. It was shown that the corneal collagen interfibrillar spacing decreased with age, and this may be related to changes in the matrix proteoglycan composition [52,53].…”

Section: Discussionmentioning

confidence: 99%

“…1, Tables 1-4), which can be attributed to differences in composition and organization of tissue matrix. In adult cornea, only 50-55% of collagens makes type I collagen [37,52,53], while in tendon 90% is type I [35,49]. Type I makes almost 90% of collagens also in meniscus [36,50].…”

Section: Discussionmentioning

confidence: 99%

Thermal stability of collagen in naturally ageing and in vitro glycated rabbit tissues

Trębacz

Szczęsna

Arczewska

2018

J Therm Anal Calorim

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The aim of the work was to compare glycation-related changes in thermal denaturation of collagen in naturally ageing and in vitro ribosylated tissues. Samples of cornea, meniscus and Achilles tendon from young adult and from ageing rabbits were compared. Moreover, in vitro glycated samples (ribose, 100 mg mL -1 , 14 days) were prepared for both age groups. Collagen in tissues was characterized in terms of thermal denaturation parameters obtained from differential scanning calorimetry. The level of pentosidine and other fluorescent advanced glycation end products (AGEs) in the papain-digested tissue samples was evaluated using spectrofluorimetry (k ex/em : 335/385 and 370/440 nm). In naturally ageing tissues, changes in properties of extracellular matrix collagen expressed in terms of parameters of thermal denaturation and fluorescent AGEs levels were tissue dependent and were related to differences in organization of matrix components. No signs of increased level of AGEs were found in the naturally ageing cornea, unlike in the tendon and meniscus. In vitro ribation proved by gains of fluorescent AGEs affected thermal stability of collagen in all tissues, both in young adult and in the ageing ones. The effects of ribation were considerably greater than the effects of natural ageing. The increase in denaturation temperature was similarly strong in all tissues and did not correlate with the increase in fluorescent AGEs. As a conclusion, parameters of collagen thermal denaturation are tissue and age dependent and an amount of fluorescent AGEs is not the only determinant of increasing thermal stability of glycated collagen.

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Ageing of the human corneal stroma: structural and biochemical changes

Cited by 245 publications

References 15 publications

Birefringence of the central cornea in children assessed with scanning laser polarimetry

Birefringence of the central cornea in children assessed with scanning laser polarimetry

The relationship between central corneal thickness and the optic disc in an elderly population: the Bridlington Eye Assessment Project

Thermal stability of collagen in naturally ageing and in vitro glycated rabbit tissues

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