Ageing‐induced changes in glutathione system of sunflower seeds

Torres, M.; Paula, M. De; Pérez-Otaola, M.; Darder, M.; Frutos, G.; Martínez-Honduvilla, C. J.

doi:10.1111/j.1399-3054.1997.tb01067.x

Cited by 22 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ϩ or Ϫ signs in boxes indicate significant (P value of Student's t test Ͻ 0.01) up-regulation (ϩ) or down-regulation (Ϫ) of the gene between control and PEG-treated embryos at that particular stage of development. anthus annuus) seeds, and seed viability was strictly dependent upon glutathione metabolism because high GR activity was observed only in those seeds with high germinability (Torres et al, 1997). A positive correlation between activity of some antioxidant enzymes and embryogenic capability was also demonstrated in white spruce cultured cells (Stasolla and Yeung, 2001).…”

Section: Discussionmentioning

confidence: 95%

The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos

et al. 2003

View full text Add to dashboard Cite

Somatic embryogenic cultures of white spruce (Picea glauca) represent a valuable system to study molecular mechanisms regulating embryo development because many embryos of defined developmental stages can be generated. The inclusion of polyethylene glycol (PEG) in the maturation medium can improve the number and quality of embryos produced. To learn more about the mechanism of action of PEG, we analyzed transcript profiles of stage-specific embryos matured without (control) or with (PEG treated) PEG. RNA extracted from maturing spruce embryos was analyzed on DNA microarrays containing 2,178 cDNAs from loblolly pine (Pinus taeda). The efficiency of heterologous hybridization between spruce and pine species on microarrays has been documented previously (L. van Zyl, S. von Arnold, P. Bozhkov, Y. Chen, U. Egertsdotter, J. MacKay, R. Sederoff, J. Shen, L. Zelena, D. Clapham [2002] Comp Funct Genomics 3: 306-318). Several pine genes, including the apparent homologs to the Arabidopsis genes ZWILLE, FIDDLEHEAD, FUSCA, and SCARECROW, increased in expression after PEG treatments. These genes are known to be involved in the formation of the embryo body plan and in the control of the shoot and root apical meristems. The increased transcript levels of these genes in immature PEG-treated embryos suggest that PEG may improve the quality of spruce somatic embryos by promoting normal differentiation of the embryonic shoot and root. Changes in the transcript levels of many genes involved in sucrose catabolism and nitrogen assimilation and utilization were also observed between control and PEG-treated embryos.Embryogenesis is a critical stage of the plant life cycle because it establishes the basic body plan. Little is known about the molecular mechanisms that regulate the process (Harada, 1999). This paucity of information is partially because of the location of the embryos, which are embedded within the maternal tissue and are difficult to dissect. The generation of embryos in culture through somatic embryogenesis has become a model system for investigating factors that affect embryo growth. Somatic embryogenesis provides a large number of embryos at defined stages of development, and allows alterations of the embryonic environment through manipulations of the culture conditions. White spruce (Picea glauca) is an economically important species in North America, utilized for pulpwood and lumber production (Hosie, 1979). Regeneration of this species via somatic embryogenesis (Hakman and Fowke, 1987;Lu and Thorpe, 1987) has represented a means of propagation and a model system for conducting physiological and biochemical studies (for review, see Stasolla et al., 2002). Generation of white spruce somatic embryos is commonly achieved by transferring embryogenic tissue onto an abscisic acid (ABA)-containing maturation medium (Lu and Thorpe, 1987). Although such embryos may appear "morphologically" mature, they do not perform well during postembryonic growth without the imposition of a drying period. Improvement of embryo quality can ...

show abstract

Section: Discussionmentioning

confidence: 95%

The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Peroxidação lipídica e 30 S. C. B. R. JoSÉ et al estresse oxidativo têm causado deterioração das sementes de oleaginosas durante seu envelhecimento. Em sementes de girassol, reduções de vigor e de viabilidade durante o envelhecimento acelerado estão associadas com a diminuição do potencial de enzimas antioxidantes e, desse modo, elevação da peroxidação lipídica (Torres et al, 1997).…”

Section: Introductionunclassified

Armazenamento de sementes de girassol em temperaturas subzero: aspectos fisiológicos e bioquímicos

José

Salomão

Costa

et al. 2010

Rev. bras. sementes

View full text Add to dashboard Cite

RESUMo -o grande avanço obtido na produção agropecuária se deve, dentre outros fatores, a capacidade brasileira de incorporar e utilizar recursos genéticos. A Embrapa Recursos Genéticos e Biotecnologia é a empresa responsável pela conservação em longo prazo de germoplasma semente, conservados a -20 °C. Para garantir a qualidade do material conservado, minimizando o processo de deterioração, é necessário que se mantenham as condições adequadas de armazenamento, realizando o manejo correto do germoplasma. os objetivos dessa pesquisa foram avaliar o efeito do teor de água nas sementes sobre a tolerância das mesmas ao armazenamento em temperaturas subzero de -20 °C e -196 °C, bem como associar essa tolerância aos aspectos fisiológicos e bioquímicos. Foi objetivo também verificar o efeito da umidificação prévia das sementes sobre a qualidade fisiológica das mesmas, após armazenamento. A qualidade das sementes foi avaliada pelos testes de condutividade elétrica, primeira contagem e contagem final do teste de germinação, e determinação do índice de peróxidos. Sementes de girassol podem ser secadas até 3,2% de teor de água em sílica gel ou em câmara de secagem e armazenadas a -20 °C ou -196 °C, sem perda de germinação e vigor. Menor deterioração das sementes, avaliada pelo índice de peróxidos, é observada em sementes armazenadas em nitrogênio líquido. O tratamento de umidificação deve ser utilizado na avaliação de plântulas na primeira contagem do teste de germinação e no teste de condutividade elétrica.Termos para indexação: germinação, vigor, conservação, nitrogênio líquido, câmara fria. SToRAGE oF SUNFLoWER SEEDS IN SUB-ZERo TEMPERATURES:PHYSIoLoGICAL AND BIoCHEMICAL ASPECTS ABSTRACT -The significant progress in Brazilian agricultural production has been due its capacity to incorporate and use genetic resources. Embrapa Genetic Resources and Biotechnology is the institution responsible for the long-term conservation of the seed germplasm of many crops, stored at -20 °C. Appropriate storage is necessary for correct germplasm management, to ensure the quality of the material stored and minimize the deterioration process. The objectives 29

show abstract

“…The scavenging of free radicals and prevention of potential molecular damages are controlled by the defence mechanisms activated in plant cells. Those mechanisms involve low molecular and enzymatic antioxidants (Bowler et al 1992;Foyer et al, 1994;Torres et al 1997;Bailly et al 1998). The main antioxidants of the water phase are: ascorbate and glutathione.…”

Section: Introductionmentioning

confidence: 99%

“…This cycle is observed not only in chloroplasts (Asada 1992), but also in the cytosol, mitochondria and peroxisomes (Potters et al 2002). It involves specific enzymes, such as ascorbate peroxidase (APO), monodehydroascorbate reductase (MR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) (Bowler et al 1992;Foyer et al 1994;Arrigoni 1994;Torres et al 1997;de Pinto et al 1999). The active cooperation of enzymes and high levels of low molecular antioxidants may prevent the loss of metabolic balance in cells, maintaining their redox status at a high level.…”

Section: Introductionmentioning

confidence: 99%

Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L.

Ratajczak

Pukacka

2011

Acta Soc Bot Pol

View full text Add to dashboard Cite

Two seed lots of Acer saccharinum (recalcitrant), with an initial moisture content of 50% and 55%, were stored at +3°C for 6 months. After this time, their viability (measured as germinability) reached 100% and 30%, respectively. In embryo axes and cotyledons extracted from seeds, two major low molecular antioxidants were assayed: ascorbate (ASA and DHA) and glutathione (GSH and GSSG); and activities of enzymes of the ascorbateglutathione cycle were measured: ascorbate peroxidase (APO) (E.C. 1.11.1.11), monodehydroascorbate reductase (MR) (E.C. 1.6.5.4), dehydroascorbate reductase (DHAR) (E.C. 1.8.5.1), and glutathione reductase (GR) (E.C. 1.6.4.2.). GSH and GSSG contents of embryo axes of stored seeds decreased, as compared to the control (fresh, non-stored seeds), but a larger decrease was observed in seeds with 30% viability. In cotyledons, a particularly high increase in the GSH content in relation to the control was observed in seeds with 100% viability, while the GSSG content was significantly lower in both stored seed lots than in the control. The ASA level was twice as high in seeds with 30% viability as in the control, both in embryo axes and in cotyledons. The activity of enzymes of the ascorbate-glutathione cycle was higher in embryo axes than in cotyledons. In embryo axes of seeds with 100% viability, enzyme activities were slightly lower than in the control, while in those of seeds with 30% viability, their activities were higher than in the control. The observed changes in activities of enzymes of the ascorbate-glutathione cycle and in ascorbate and glutathione levels suggest that the stored seeds of A. saccharinum have an active antioxidant system, which plays an important role in maintaining their viability during storage.

show abstract

Ageing‐induced changes in glutathione system of sunflower seeds

Cited by 22 publications

References 21 publications

The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos

The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos

Armazenamento de sementes de girassol em temperaturas subzero: aspectos fisiológicos e bioquímicos

Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L.

Contact Info

Product

Resources

About