Ageing in Werner syndrome

Goto, Makoto; Iwaki-Egawa, Sachiko; Watanabe, Yasuhiro

doi:10.5582/bst.2012.v6.1.33

Cited by 10 publications

(13 citation statements)

References 47 publications

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“…22) According to free radical theory of aging, senescence is the result of oxidative stress. 23) A large number of studies revealed that the balance between ROS system and antioxidation system determines the degree of oxidative stress.…”

Section: ) D-galmentioning

confidence: 99%

Effect of Colla corii asini (E’jiao) on D-Galactose Induced Aging Mice

Wang

Liu

Cao

et al. 2012

Biological & Pharmaceutical Bulletin

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Colla corii asini (E'jiao), donkey-hide gelatin prepared by stewing and concentrating from Equus asinus L. donkey hide, is a traditional Chinese medicine preparation widely used in clinical hematic antanemic therapy in China. The aim of the present study was to investigate potential anti-aging effect of Colla corii asini and explore related mechanisms in D-galactose (gal) induced aging model mice. The mice were artificially induced aging by subcutaneously injection with D-gal at the dose of 100 mg/kg·d for 8 weeks. Colla corii asini was simultaneously treated to them once daily by intragastric gavage. Appetite, mental condition, body weight, and organ index were observed. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as levels of malondialdehyde (MDA) in serum, brain, and liver were determined by according assay kits. Western blotting analysis was used to detect p16 and p21 expression. Results indicated that Colla corii asini could improve appetite, mental condition, body weight, and organ condition of model mice, improve SOD, CAT, and GSH-Px activities, reduce MDA levels, and modulate agerelated genes expression in D-gal induced mice. Therefore, Colla corii asini may have effect to suppress the aging process through enhancing antioxidant activity, scavenging free radicals, and modulating aging-related gene expression.Key words Colla corii asini (E' jiao); anti-aging; D-galactose; antioxidant; aging-related gene Aging is a natural phenomenon, and it is always associated with diverse chronic diseases, including cancer, Parkinson's and cardiovascular diseases, etc.1,2) Anti-aging has already become a major public issue with the increasing elderly population in the world. The free radical theory of aging was conceived by Harman in 1956. 3) Abundant evidences suggest that oxidative stress plays a central role in the process of biological aging.4) Oxygen-derived free radicals exert detrimental effects on human, including peroxidation of membrane lipids, enzyme inactivation, DNA fragmentation, and activation of apoptosis.5) In addition, supplementation with antioxidants has been reported to be beneficial with respect to slowing this aging process.D-Galactose (D-gal) has been used to induce oxidative stress in vivo to mimic the natural aging in mice. D-Gal can be metabolized at normal concentration, but when at high levels, it can be converted into galactitol under the catalysis of gal oxidase, resulting in the generation of superoxide anions and oxygen-derived free radicals.6) As one of the antioxidant defense systems, a group of enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), act as superoxide anion and H 2 O 2 scavengers to prevent reactive oxygen species (ROS)-induced damage, which may cause the changes of some biomarkers.7) Malondialdehyde (MDA) is a major biomarker that appears during the final stages of lipid peroxidation initiated by excessive ROS. An increase in the hepatic MDA concentration suggests th...

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Section: ) D-galmentioning

confidence: 99%

Effect of Colla corii asini (E’jiao) on D-Galactose Induced Aging Mice

Wang

Liu

Cao

et al. 2012

Biological & Pharmaceutical Bulletin

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show abstract

“…Insulin resistance seems to occur first, followed by oxidative stress. Moreover, it has been observed that individuals with WS exhibit high insulin resistance because of the accumulation of visceral fat tissue; high oxidative stress in the fibroblasts of these individuals due to the instability of mitochondrial functions and low copper‐ and zinc‐containing superoxide dismutase and manganese‐containing superoxide dismutase activities have also been observed . Therefore, individuals with WS may have a genetic predisposition to NAFLD and NASH.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin Improves Nonalcoholic Fatty Liver Disease in Werner Syndrome with Diabetes Mellitus

Takemoto

Yamaga

Furuichi

et al. 2015

J American Geriatrics Society

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“…So far, the FRET approach has been well accommodating to examining various peptidases, and one case in point is the development of fluorescent probes of the matrix metalloproteinases (MMPs). MMPs are well recognized to be critical in normal tissue remodeling as well as in diverse pathological states, such as lung diseases, atherosclerosis, and carcinoma . Among the MMP family, MMP‐7 has been a persuasive target due to its overexpression in pancreatic, colon, and breast cancers…”

Section: Fluorescence Resonance Energy Transfer Probesmentioning

confidence: 99%

“…MMPs are well recognized to be critical in normal tissue remodeling as well as in diverse pathological states, such as lung diseases, atherosclerosis, and carcinoma. 61,71,72 Among the MMP family, MMP-7 has been a persuasive target due to its overexpression in pancreatic, colon, and breast cancers. 73 Bearing in mind the endopeptidase activity of MMP-7, Tung and co-workers developed a FRET probe consisting of a enzyme-selective peptide VPLSLTMG, an NIR fluorescence donor (Cy5.5), and an NIR fluorescence absorber (NIRQ820).…”

Section: Fluorescence Resonance Energy Transfer Probesmentioning

confidence: 99%

Strategies in the Design of Small‐Molecule Fluorescent Probes for Peptidases

Chen

et al. 2014

Medicinal Research Reviews

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Peptidases, which can cleave specific peptide bonds in innumerable categories of substrates, usually present pivotal positions in protein activation, cell signaling and regulation as well as in the origination of amino acids for protein generation or application in other metabolic pathways. They are also involved in many pathological conditions, such as cancer, atherosclerosis, arthritis, and neurodegenerative disorders. This review article aims to conduct a wide-ranging survey on the development of small-molecule fluorescent probes for peptidases, as well as to realize the state of the art in the tailor-made probes for diverse types of peptidases.

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Ageing in Werner syndrome

Cited by 10 publications

References 47 publications

Effect of <i>Colla corii asini</i> (<i>E’jiao</i>) on <small>D</small>-Galactose Induced Aging Mice

Effect of <i>Colla corii asini</i> (<i>E’jiao</i>) on <small>D</small>-Galactose Induced Aging Mice

Astaxanthin Improves Nonalcoholic Fatty Liver Disease in Werner Syndrome with Diabetes Mellitus

Strategies in the Design of Small‐Molecule Fluorescent Probes for Peptidases

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