Ageing and colony‐forming efficiency of human hair follicle keratinocytes

Lecardonnel, Jennifer; Deshayes, Nathalie; Genty, Gaïanne; Parent, N; Bernard, Bruno; Rathman-Josserand, Michelle; Paris, Maryline

doi:10.1111/exd.12204

Cited by 12 publications

(12 citation statements)

References 19 publications

(13 reference statements)

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“…These specific mechanisms are complex, and the key growth factors involved in these communications are still matter of studies . With ageing, a cumulative oxidative stress is described to affect these hair compartments , leading to the loss of stem cell properties and to a progressive decrease in the hair density. Maintaining these properties by protecting the hair cells is thus a key to maintain a full hair density.…”

Section: Introductionmentioning

confidence: 99%

A combination of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol stabilizes hypoxia‐inducible factor 1‐alpha and improves hair density in female volunteers

Juchaux

Sellathurai

Perrault

et al. 2020

Intern J of Cosmetic Sci

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Objective The aim of this study was first to demonstrate that a combination of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol could synergize in vitro on biological pathways associated with hair growth and then to demonstrate the benefit on hair density in a clinical study. Methods The effects of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol directly on the hypoxic inducible factor‐1α protein (HIF‐1α) and related genes expression were demonstrated on keratinocytes in culture in vitro using western‐blot analysis and real time quantitative polymerase chain reaction analysis. The effect of resveratrol against oxidative stress induced by hydrogen peroxide treatment was studied in hair follicle and hair matrix cells in vitro using the sensitive probe Dichloro‐dihydro‐fluorescein diacetate (DCFH‐DA). Finally, a randomized clinical study on hair density was conducted on 79 Caucasian female subjects to assess the effect of this combination of actives. Results Pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol stabilized HIF‐1a protein and increased the expression of HIF‐1α target genes. Resveratrol significantly reduced the oxygen peroxide‐induced oxidative stress generated in hair follicle and hair matrix cells. The clinical study showed that a topical treatment with the combination significantly increased the hair density on women from 1.5 months. Conclusion In addition to the antioxidant properties of resveratrol, the association of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol revealed a synergistic effect on the HIF‐1α pathway. The results of the clinical study confirmed the importance of such a combination to increase the hair density.

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Section: Introductionmentioning

confidence: 99%

A combination of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol stabilizes hypoxia‐inducible factor 1‐alpha and improves hair density in female volunteers

Juchaux

Sellathurai

Perrault

et al. 2020

Intern J of Cosmetic Sci

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“…Decline of tissue regenerative potential is a hallmark of aging, and this could be ascribed to age‐related changes in tissue‐specific stem cells . Epidermal and dermal stem cell compartments are therefore fundamental for the continuous renewal of the skin, and their malfunctioning could be detrimental for the tissue, facilitating and/or accelerating irreversible aging process . In particular, adult stem cells are the unique source for skin tissue renewal .…”

Section: Introductionmentioning

confidence: 99%

Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro‐collagen lipopeptide, creatine, and urea on skin aging signs

Sanz

Campos

Milani

et al. 2015

J of Cosmetic Dermatology

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SummaryBackground: Epithelial regeneration in skin is achieved by the constant turnover and differentiation of keratinocytes. Epidermal and dermal stem cells compartments are fundamental for the continuous renewal of the skin. Adult stem cells are the unique source for skin tissue renewal. Plants have stem cells and plant derived stem cell extracts are now used in topical products for their potential anti-ageing and antiwrinkle effects. A new dermocosmetic product containing apple stem cell extract, urea, creatine and palmitoyl tripeptide-38 (Ureadin Fusion Serum Lift Antiarrugas, ISDIN S.A), has been recently developed to target different aspects involved in skin aging. Study aim: To assess in vitro the effects of this new serum on the metabolic functions of human senescent fibroblasts and in vivo the anti-aging effects by clinical and instrumental evaluation. Methods: We evaluated the effects of the serum on the mitochondrial ROS (reactive oxygen species) production in human senescent cultured fibroblasts measured at 0.1% and 1% using the Mitoread AntiOx mtROS method. In addition we evaluated the anti-ageing in vivo effect of this new serum applied on the face twice daily for 28 consecutive days and assessed by clinical and instrumental evaluation in 32 women with sensitive skin bearing wrinkles on crow's feet. Results: The tested serum both at 0.1% and 1% induces a significant increase in 02 consumption, cellular ATP level and a reduction in extra-cellular lactate concentration. The product reduces also significantly the mitochondrial ROS production. The clinical study shows a relevant anti-wrinkle effect in 71% of the treated women with visible effects in 68% of the subjects as soon as 7 days of treatment. A significant increase in dermal density and skin elasticity was also observed. Conclusion: The use of this novel anti-aging serum demonstrated a significant improvement of aging skin signs with first visible results achieved after one week of use. The product seemed to optimize the metabolic functions in human senescent cultured fibroblast restoring a more efficient cell metabolism therefore contributing to the anti-aging properties of the product.

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“…In parallel, the ability to in vitro give rise to holoclones progressively decline while the number of paraclones increases . Of note, in human hair follicles, an age‐related progressive decline in holoclone production was also observed . Altogether, these results suggest an age‐related progressive loss of human epidermal stem cells' functionality.…”

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confidence: 58%

Glycobiology to the rescue !

Bernard

2016

Experimental Dermatology

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Epidermal homoeostasis is maintained by a fine balance of keratinocyte proliferation and terminal differentiation. In skin homoeostasis, cells which enter the differentiation programme must be compensated by cell replenishment in the epidermal basal layer. Today, it is admitted that two distinct proliferative cells coexist in the basal layer of epidermis, namely slow-cycling stem cells and committed progenitor 4Of note, the basement membrane and the uppermost dermis are key components of the epidermal stem cells' niche. 5From a histological point of view, skin ageing is mainly characterized by a flattening of the dermal-epidermal junction, an epidermal thinning in some body sites like scalp, a decreased basal cell density, a delamination of the basement membrane and alteration of the superficial dermis. 6 Of note, the most striking alterations reported so far deal with extracellular matrix and more precisely, with decreased expression of glycosaminoglycans and related proteoglycans. In parallel, the ability to in vitro give rise to holoclones progressively decline while the number of paraclones increases. 4 Of note, in human hair follicles, an age-related progressive decline in holoclone production was also observed. 9 Altogether, these results suggest an agerelated progressive loss of human epidermal stem cells' functionality. More generally speaking, glycans which are endowed with a unique and huge molecular diversity, represent the third language of life, besides nucleic acids and proteins, 12 and as such, must be taken into consideration more fully as key actors of skin biology and important factors in investigative dermatology. Considering the molecular

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Ageing and colony‐forming efficiency of human hair follicle keratinocytes

Cited by 12 publications

References 19 publications

A combination of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol stabilizes hypoxia‐inducible factor 1‐alpha and improves hair density in female volunteers

A combination of pyridine‐2, 4‐dicarboxylic acid diethyl ester and resveratrol stabilizes hypoxia‐inducible factor 1‐alpha and improves hair density in female volunteers

Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro‐collagen lipopeptide, creatine, and urea on skin aging signs

Glycobiology to the rescue !

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