Aged Rats Are Susceptible to Nephrotoxicity Induced by Iodinated Contrast Media

Duan, Shao‐Bin; Liu, Gai-Ling; Chen, Guo-Chun; Wang, Pian; Pan, Peng; Xu, Xin

doi:10.3109/0886022x.2012.741650

Cited by 8 publications

(14 citation statements)

References 29 publications

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“…Histological changes were observed and scored semiquantitatively in a blind manner using an arbitrary scale. 12 A minimum of 10 fields at 200 magnification were assessed and graded in renal cortex of each biopsy tissue. For tubular injury, the following score was used: 0 ¼ no tubular injury; 1 ¼525% of tubules injured; 2 ¼ from 25% to 50% of tubules injured; 3 ¼ from 51 to75% of tubules injured; 4 ¼ more than 76% of tubules injured.…”

Section: Renal Histopathological Examinationmentioning

confidence: 99%

“…of the CM (diatrizoate meglumine) with 300 mg I/mL were given through a caudal vein in 2 min to induce CIAKI; [9][10][11][12] at the same time, the other two groups were given equal volume of normal saline, 24 h before and after CM injection and half hour just before CM administration, the rats of group HM and HS were given injection of MTP131 or SPI 20 (3 mg/kg) into peritoneal cavity for 3 times, rats from group NN, HN, and HH were also given equal volume of normal saline. All rats were maintained in individual metabolic cages with free access to water to collect 24-h urine samples.…”

Section: Experimental Protocolmentioning

confidence: 99%

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Mitochondria-targeted peptides prevent on contrast-induced acute kidney injury in the rats with hypercholesterolemia

et al. 2013

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Objective: The objective of this study is to evaluate the effect and mechanism of mitochondriatargeted peptides (MTP131 and SPI20) on contrast-induced acute kidney injury (CI-AKI) in rats with hypercholesterolemia. Method: Forty SD rats were randomly divided into normal diet group (NN, n ¼ 8) and high cholesterol supplemented dietary group (HN, n ¼ 32). At the end of 8 weeks, the group HN was divided into four subgroups. All Rats were given injection of either diatrizoate (10 mL/kg) or equal volume of normal saline, the rats pretreated with MTP131 or SPI20 were given injection with MTP131 or SPI 20 (3 mg/kg) by peritoneal cavity for 3 times. Blood, urine and renal tissue samples were prepared to determine biochemical parameters. The renal pathological changes were evaluated by hematoxylin and eosin staining and scored semiquantitatively, The protein expression of renal NOX4 was also measured by Western blotting. Results: In diatrizoate-injected rats, Serum creatinine (Scr), fractional excretion of sodium (FeNa%), fractional excretion of potassium (FeK%), pathological scores, renal malondialdehyde (MDA) content, the NADPH oxidase activity and the expression of NOX4 in kidney tissue were significantly increased (p50.01). In the groups pretreated with MTP131 or SPI20, the levels of Scr, FeNa%, FeK%, MDA content and NADPH oxidase activity in renal tissue decreased (p50.01), the levels of renal super oxygen dehydrogenises and ATPase activity increased (p50.01). The renal injuries induced by contrast media (CM) were alleviated. Conclusion: MTP131 and SPI20 might protect acute kidney injury induced by CM in rats with hypercholesterolemia.

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Section: Renal Histopathological Examinationmentioning

confidence: 99%

Section: Experimental Protocolmentioning

confidence: 99%

Mitochondria-targeted peptides prevent on contrast-induced acute kidney injury in the rats with hypercholesterolemia

et al. 2013

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“…Several models reported dehydrating the rats for 24 to 48 hours prior to contrast administration (31)(32)(33)(34)(35)(36)(37). Efrati et al demonstrated that the renal physiological changes in response to CM are most pronounced in the dehydrated animal (38).…”

Section: Pre-test Conditionsmentioning

confidence: 99%

“…Urinary markers included urinary creatinine and sodium, which were generally evaluated with commercial spectrophotometric kits (32,33,60). Urinary neutrophil gelatinase-associated lipocalin and KIM-1 are relatively new biomarkers of acute kidney injury (63,64).…”

Section: Biochemical Studies and Renal Function Assessmentmentioning

confidence: 99%

“…The degree of intracellular oxidative stress can be determined by quantifying malondialdehyde (MDA), and the activity of antioxidants such as superoxide dismutase (SOD), renal tissue catalase (CAT) and glutathione peroxidase (GSH-Px) (32,33,36,58). The assessment of each of these variables has been validated in in-vivo and in-vitro models.…”

Section: Assessment Of Oxidative Stressmentioning

confidence: 99%

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Experimental rat models for contrast-induced nephropathy; a comprehensive review

et al. 2020

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Contrast-induced nephropathy (CIN) is an iatrogenic disease caused by the parenteral administration of iodinated contrast media (CM). A number of agents are currently being assessed to minimise or prevent CIN. Such agents are typically assessed using rat models. The aim of this study was to provide a comprehensive review of the rat models of CIN used in pre-clinical research. The MEDLINE, EMBASE, Web of Science and Cochrane databases were systematically searched. Articles reporting rat models of CIN were included for assessment. Study designs, contrast agents and outcome measures were assessed. Of the assessed studies, a majority report a requirement for pre-existing renal impairment prior to the administration of CM. Outcome measures are heterogenous between studies, but typically include assessment and quantification of serum biochemical markers, cellular oxidative stress and histopathological changes. The significant variation in methodology reported in the current literature highlights the lack of consensus. The use of a reliable pre-contrast insult appears critical to result in the development of contrast nephropathy. The use of acceptable outcome measures appears to include serum laboratory markers, quantification of reactive oxygen species (ROS) and objective histopathological outcomes. ABSTRACT Implication for health policy/practice/research/medical education:Contrast-induced nephropathy (CIN) is an iatrogenic disease caused by the parenteral administration of iodinated contrast media. A number of agents are currently being assessed to minimise or prevent CIN, of which initial assessment of these agents are typically assessed using rat models. We provide a comprehensive review outlining previously utilised rat models for the assessment of CIN. This paper is of benefit, as it highlights the different approaches to studying this model and provides a framework for future trials assessing novel agents. Please cite this paper as: Perera M, Ischia J, Bolton D, Shulkes A, Baldwin GS, Patel O. Experimental rat models for contrast-induced nephropathy; a comprehensive review. J Nephropathol. 2020;9(2):e12.

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Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway

Wang

Zhou

et al. 2017

International Immunopharmacology

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Aged Rats Are Susceptible to Nephrotoxicity Induced by Iodinated Contrast Media

Cited by 8 publications

References 29 publications

Mitochondria-targeted peptides prevent on contrast-induced acute kidney injury in the rats with hypercholesterolemia

Mitochondria-targeted peptides prevent on contrast-induced acute kidney injury in the rats with hypercholesterolemia

Experimental rat models for contrast-induced nephropathy; a comprehensive review

Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway

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