Aged black garlic extract induces inhibition of gastric cancer cell growth in�vitro and in�vivo

Abstract

Abstract. There is mounting evidence that garlic extracts possess significant anticancer actions. However, no studies have been reported on the effects of aged black garlic extracts (ABGE) on gastric cancer in vitro or in vivo. To examine the potential action of ABGE against gastric cancer, the present study evaluated its effect on the inhibition of cell proliferation and induction of apoptosis in SGC-7901 human gastric cancer cells. Additionally, we performed an in vivo study by inoculating the murine foregastric carcinoma cell line in Kunming mice and treating them with various doses of ABGE (0, 200, 400 and 800 mg/kg, intraperitoneally) for 2 weeks. Dosedependent apoptosis was detected in ABGE-treated cells in in vitro studies. In tumor-bearing mice, significant antitumor effects of ABGE were observed, such as growth inhibition of inoculated tumors. Further investigation of serum superoxide dismutases, glutathione peroxidase, interleukin-2 and the increased indices of spleen and thymus indicated that the anticancer action of ABGE may be partly due to its antioxidant and immuno modulative effects.

Effects of aged garlic extract and endurance exercise on skeletal muscle FNDC-5 and circulating irisin in high-fat-diet rat models

“…Aged garlic extract (AGE) is well-documented alternative source of garlic that is odorless and rich in antioxidants [9]. Moreover, it has been reported that AGE may play a role in the mitigation of cardiovascular disease [10,11], inflammation [12], hypertension [13], and cancer risk [14]. To examine the effects of AGE and exercise as a combined treatment, we recently performed a study of the efficacy of AGE and exercise interventions in HFD inducedobesity.…”

Induction of apoptosis by a hexane extract of aged black garlic in the human leukemic U937 cells

“…Aged black garlic has been generally reported to have excellent antioxidant potency when compared to fresh or cooked garlic. The reason is probably that the bioactivity of some natural products increases during the process of fermentation [17][18][19][20][21][22][23], but no specific experimental proof has yet established its anti-cancer efficacy. In this study, we have demonstrated that HEABG significantly decreased cell proliferation in human leukemic U937 cells by inducing apoptosis, as determined by DAPI staining, agarose gel electrophoresis and flow cytometric analyses.…”

“…One of the most useful garlic preparations is aged black garlic, which is produced by aging whole garlic at high temperature and humidity [17]. Previous studies have shown that the extracts of aged black garlic have many pharmacological activities, including antioxidant, radical scavenging and anti-cancer effects [17][18][19][20][21][22]. Recently, Kim et al [23] reported that a hexane extract of aged black garlic (HEABG) potently suppressed the proliferation of tumor necrosis factor (TNF)-α-activated human endometrial stromal cells, through inhibiting the activation of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) transcription factors.…”

Parameter Fisikokimia dan Analisis Kadar Allyl Disulfide dalam Ekstrak Etanol 70% Bawang Putih (Allium sativum L.) dengan Perbandingan Daerah Tempat Tumbuh

“…Berbagai penelitian farmakologi tentang bawang putih telah banyak dilakukan, tidak hanya secara in vivo (dengan hewan percobaan) tetapi juga in vitro (dalam tabung kultur). Berbagai penelitian yang telah dikembangkan menunjukkan bahwa bawang putih mempunyai efek biologis dan farmakologis seperti antitumorigenesis, antianteroskleriosis, modulasi gula darah dan antibiosis, penghambatan pertumbuhan kanker (Wang et al, 2011), antihipertensi (Qidwai et al, 2000), aktivitas anti-oksidatif (Borek, 2001), antibakteri (dari isolasi jamur endofit pada bawang putih) (Hidayanti 2010), mencegah perkembangan metastasis tumor (Hu et al, 2002), peningkatan aktivitas fibrinolisis (Pizorno dan Murray, 2000), menghambat pertumbuhan bakteri Salmonella enteritidis, Staphylococcos aureus, dan Salmonella typhosa (Poeloengan, 2001). Selain itu, bawang putih juga dapat menurunkan gejala aflatoksin (Maryam et al, 2003).…”