Age-related tissue distribution of swine Torque teno sus virus 1 and 2

Aramouni, Mario; Segalés, Joaquím; Cortey, Martı́; Kekarainen, Tuija

doi:10.1016/j.vetmic.2010.05.036

Cited by 60 publications

(52 citation statements)

References 28 publications

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“…These results indicate that TTSuV infection has spread to pigs of all production stages. Finisher pigs had significantly higher rates of TTSuV detection than animals from the other stages, in agreement with previous studies that have reported that TTSuV infection increases with the age of the animals (Sibila et al 2009b, Aramouni et al 2010). …”

Section: Discussionsupporting

confidence: 92%

“…Studies have demonstrated the presence of TTSuV in the serum and organs of pigs of different ages (Kekarainen & Segalés 2012) and have shown that the prevalence increases with the age of the animals (Sibila et al 2009b, Aramouni et al 2010. In Brazil, TTSuV infection has been identified in suckling piglets (Leme et al 2012) and slaughter-age pigs (Leme et al 2013), and in the reproductive tracts of boars and sows (Ritterbusch et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Leme

Alfieri

2013

Pesq. Vet. Bras.

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Pesq. Vet. Bras. 33 (7) It has been demonstrated that TTSuV might increase the severity of other important viral diseases with economic and public health impacts. At present, there is no information on the age distribution of pigs infected with TTSuV in Brazilian herds. This study evaluated the frequency of TTSuV infection in pigs at different stages of production. Fecal samples (n=190) from pigs at 1 to 24 weeks of age and from breeders at 6 farrow-to-weaning (up to 8 weeks of age) and 9 grower-to-finish (9 weeks of age onwards) farms in the western region of Paraná state, Brazil, were evaluated by PCR. Fragments of the 5' UTRs of TTSuV1 and/or TTSuVk2 DNAs were identified in 126 (66.3%) of the fecal samples. Significant differences were found with the percentages of positive samples for TTSuV1, TTSuVk2, and mixed infections by both genera between and within the different pig production stages. Fecal samples from the grower-to-finish farms had TTSuV detection rates (90.1%; 64/71) that were significantly (p<0.05) higher than those from the farrow-to-weaning farms (52.1%; 62/119). TTSuV detection was significantly (p<0.05) more frequent in finisher pigs than in the animals from the other stages. The UTR nucleotide sequences in this study presented higher similarities to strains from Norway (96%, TTSuV1), and Argentina and China (97.1%, TTSuVk2). These results suggest that TTSuV infection has spread to pigs of all production stages and that the viral infection rate increases with the age of the animals. In the western region of Paraná state, Brazil, TTSuV1 and TTSuVk2-induced infections were more frequently observed in suckling piglets and finisher pigs, respectively. Phylogenetic analysis pointed out the possibility of different strains of TTSuV1 and TTSuVk2 circulating in pig herds of Brazil. há informações sobre a distribuição da infecção pelo TTSuV, de acordo com a faixa etária, em rebanhos suinícolas brasileiros. Este estudo avaliou a frequência da infecção pelo TTSuV nas diferentes categorias de produção de suí-nos. Amostras fecais (n=190) de suínos com 1 a 24 semanas de idade e de reprodutores provenientes 6 unidades produtoras de leitão (até 8 semanas de idade) e 9 unidades de terminação (9 semanas de idade em diante) da região oeste do Paraná, Brasil, foram avaliadas pela técnica de PCR. Fragmentos da região 5' UTR do DNA do TTSuV1 e/ ou TTSuVk2 foram identificados em 126 (66,3%) amostras fecais. Diferenças significativas foram encontradas em relação às porcentagens de amostras positivas para o TTSuV1, Pesq. Vet. Bras. 33 (7)

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Leme

Alfieri

2013

Pesq. Vet. Bras.

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“…The faecal TTSuVk2 load was significantly higher in pigs with diarrhea in the nursery age group (P < 0.01); this group also exhibited a significantly higher faecal PCV2 load (P < 0.01) compared to the non-diarrheic animals from the same age group (Table 1). This higher faecal TTSuVk2 load exhibited a pattern similar to that which was previously observed for TTSuVs in sera; i.e., higher serum TTSuVk2 but not TTSuV1 viral loads was observed in the sera of PMWS-affected pigs (Aramouni et al 2010). The pathogenic role of TTSuV has not yet been demonstrated, and its role during co-infection with other pathogens is under debate ).…”

Section: Discussionsupporting

confidence: 78%

Torque teno sus virus 1 and 2 viral loads in faeces of porcine circovirus 2-positive pigs

et al. 2015

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Recently, studies have suggested an association between the Torque teno sus virus (TTSuV) and the Porcine circovirus-2 (PCV2) in PCV2-associated disease cases. The aim of this study was to verify TTSuVs loads in pig faeces from PCV2-positive animals with and without diarrhea from PCVADaffected and PCV2-unvaccinated herds. A total of 80 faecal samples were collected individually from nursery and grow-finish pigs with (n = 40) or without (n = 40) diarrhea. The samples were tested for PCV2 and TTSuVs by using DNA binding dye SYBR Green quantitative polymerase chain reaction (qPCR). Torque teno sus virus k2 (TTSuVk2) load in the faeces was significantly higher in the nursery pigs with diarrhea, and these pigs also exhibited significantly higher PCV2 (P < 0.01) faecal matter loads compared to the non-diarrheic animals from the same age group. Torque teno sus virus 1 (TTSuV1) viral loads were the same regardless of age group and disease condition. There were no correlations between PCV2 and TTSuV1 or TTSuVk2 and TTSuV1 viral loads; however, a weak correlation (r = 0.23, P = 0.03) was found between TTSuVk2 and PCV2 viral loads. In conclusion, TTSuVk2 viral loads were significantly higher in the diarrheic faeces from the nursery pigs. Additionally, the higher loads of PCV2 and TTSuVk2 in the nursery-diarrheic animals revealed that diarrhea might have an important role in the spread of both viruses in herds.

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“…Few other cross-sectional studies [1,27] including our recent publication [29] have detected the two virus types in sera of pigs from the last stage of their growing-finishing period. In the present study, TTSuV1 and TTSuV2 nucleic acids were detected from 14 days to 150 days of age in both sera and PBMCs samples, further confirming the lifelong presence of the two virus types in pigs.…”

Section: Ttsuv2mentioning

confidence: 99%

“…Limited longitudinal studies employing serum [17,26] and cross-sectional studies employing serum and tissues [1,8,9,27] have shown that TTSuV infection increases with age. However, little is known about primary infection with TTSuV and sites of viral persistence and reactivation after infection in the pigs, and to the best of our knowledge there is no report on the detection of TTSuVs in peripheral blood mononuclear cells (PBMCs) in pigs.…”

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confidence: 99%

Infection Dynamics of Torque Teno Sus Virus Types 1 and 2 in Serum and Peripheral Blood Mononuclear Cells

Tshering

Takagi

Deguchi

2012

The Journal of Veterinary Medical Science

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ABSTRACT. This study was carried out to investigate the presence of Torque teno sus virus types 1 (TTSuV1) and 2 (TTSuV2) in a longitudinally (14 to 150 days of age) collected paired pooled sera (pSE) and peripheral blood mononuclear cells (pPBMCs) using nested polymerase chain reaction. The detection rate of TTSuV1 in pSE increased from 14 to 90 days of age, but a progressive decline was observed from 120 to 150 days of age, while in pPBMC, a high value was maintained till the end of growing-finishing period. On the contrary, except in PBMCs at 30 days of age, high detection rates of TTSuV2 were found in both pSE and pPBMCs in all sampling ages. The detection rate of TTSuVs between pSE and pPBMCs was positively correlated at all sampling ages except for TTSuV1 at 150 days of age. This is the first study showing the presence of TTSuVs in PBMCs from pigs and describing the in vivo infection dynamics of TTSuV in paired sera and PBMCs during the entire growing and finishing periods of pigs reared in conventional farms.

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Age-related tissue distribution of swine Torque teno sus virus 1 and 2

Cited by 60 publications

References 28 publications

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Torque teno sus virus 1 and 2 viral loads in faeces of porcine circovirus 2-positive pigs

Infection Dynamics of <i>Torque Teno Sus Virus</i> Types <i>1</i> and <i>2</i> in Serum and Peripheral Blood Mononuclear Cells

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