Age-related changes of platelet and plasma coagulation parameters in young pigs

Abstract

Abstract. The literature on hemostatic processes in swine is sparse and often fragmentary; hence, we conducted our study to characterize age-related changes in selected parameters of primary and secondary hemostasis in 50 growing pigs between day 2 and week 24 of age. We measured platelet count (PLT), mean platelet volume, platelet-to-large cell ratio, prothrombin time, activated partial thromboplastin time (aPTT), thrombin time (TT), and fibrinogen concentration. Among primary hemostasis parameters, PLT underwent the largest fluctuation with the animals' age, ranging from 340 to 730 × 10 9 /L. However, statistical significance was only detected for 4-week-old piglets compared to 18-week-old animals. Of the secondary hemostasis parameters measured, TT and aPTT were the most changeable. Activated partial thromboplastin time displayed a characteristic biphasic course, being relatively short before week 5 of age (17.8-19.9 s) and then becoming much longer (28.7-52.5 s). The aPTTs measured in animals 6 weeks of age and older were statistically different (p < 0.01) from those in younger piglets. The 2 main components of hemostasis, platelet hemostasis and plasma coagulation, did not develop at the same time. It took much longer for secondary hemostasis to stabilize, whereas platelet parameters were stable early in life.

Safety Evaluation of a Standardized &lt;i&gt;Macleaya cordata&lt;/i&gt; Extract in a Ninety Day Feeding Study in Weaned Piglets

“…In addition, the variations in platelet count were similar to the fluctuation in control or pre-study values, and were of a magnitude of change commonly observed in weaned piglets under similar study conditions [21].…”

A gottingen minipig model of radiation-induced coagulopathy

“…The minipig has been proposed as an alternative large animal model to the canine and NHP for evaluation of medical countermeasures for treatment of acute radiation syndrome. Development of a coagulopathy-related model would have particular utility in characterizing the underlying mechanisms associated with coagulopathy (Olsen et al 1999;Pliszczak-Krol et al 2016) and associated multi-organ dysfunction (Gando 2010) as well as hemorrhage. This study was conducted as part of the overall BARDA consortium approach to evaluate multiple circulating markers associated with coagulopathy following total body irradiation of the Gottingen minipig.…”

Impact of Embryo Transfer on the phenotype of the offspring in pigs

“…Pliszczak-Król et al [38] described that PLT variability in piglets may be due to the rapid growth of these animals and the maturation of their hematopoietic system. Despite no differences being found in the PLT concentration, signi cantly lower values were found on day 3 in PCT and MPM for males from ET compared to those derived by AI.…”