Age-related changes of p75 Neurotrophin receptor-positive adipose-derived stem cells

Yamada, Takaaki; Akamatsu, Hirohiko; Hasegawa, Seiji; Yamamoto, Naoki; Yoshimura, Tomohisa; Hasebe, Yuichi; Inoue, Yu; Mizutani, Hiroshi; Uzawa, Tohru; Matsunaga, Kayoko; Nakata, Satoshi

doi:10.1016/j.jdermsci.2010.02.003

Cited by 33 publications

(27 citation statements)

References 42 publications

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“…31,32 This dwindling osteogenic potential in hASCs with age has not been mechanistically defined, but does not appear to be due to a loss of osteoprogenitor cell numbers (as demonstrated by clonal analyses). 30 It is intriguing to speculate whether a decrease in endogenous Hedgehog signaling accompanies the decreasing osteogenic potential of hASCs with age.…”

Section: Discussionmentioning

confidence: 98%

Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells

James

Leucht

Levi

et al. 2010

Tissue Engineering Part A

137

128

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Section: Discussionmentioning

confidence: 98%

Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells

James

Leucht

Levi

et al. 2010

Tissue Engineering Part A

137

128

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“…Adipose tissue is an abundant tissue in the body and contains a stromal fraction rich in stem-progenitor cells capable of undergoing differentiation into osteogenic, chondrogenic, and adipogenic lineages (Yamada et al 2010;Sofroniew et al 2001). The in vitro as well as in vivo properties of adipose-derived stromal cells (ASCs) resemble those of MSCs obtained from bone marrow, and the liposuction procedure employed to harvest ASCs is minimally invasive for the patient.…”

Section: Introductionmentioning

confidence: 99%

“…Within in vitro cultures of ASCs, a specific p75 ? population has been purified, the behavior of which resembles that of Schwann cells (Yamada et al 2010); these cells can form myelin-like structures in vitro with PC12 cell neurites (Xu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Predifferentiated Adipose-Derived Stromal Cells for the Treatment of Spinal Cord Injury

et al. 2011

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Adipose-derived stromal cells (ASCs) are an alternative source of stem cells for cell-based therapies of neurological disorders such as spinal cord injury (SCI). In the present study, we predifferentiated ASCs (pASCs) and compared their behavior with naïve ASCs in vitro and after transplantation into rats with a balloon-induced compression lesion. ASCs were predifferentiated into spheres before transplantation, then pASCs or ASCs were injected intraspinally 1 week after SCI. The cells' fate and the rats' functional outcome were assessed using behavioral, histological, and electrophysiological methods. Immunohistological analysis of pASCs in vitro revealed the expression of NCAM, NG2, S100, and p75. Quantitative RT-PCR at different intervals after neural induction showed the up-regulated expression of the glial markers NG2 and p75 and the neural precursor markers NCAM and Nestin. Patch clamp analysis of pASCs revealed three different types of membrane currents; however, none were fast activating Na(+) currents indicating a mature neuronal phenotype. Significant improvement in both the pASC and ASC transplanted groups was observed in the BBB motor test. In vivo, pASCs survived better than ASCs did and interacted closely with the host tissue, wrapping host axons and oligodendrocytes. Some transplanted cells were NG2- or CD31-positive, but no neuronal markers were detected. The predifferentiation of ASCs plays a beneficial role in SCI repair by promoting the protection of denuded axons; however, functional improvements were comparable in both the groups, indicating that repair was induced mainly through paracrine mechanisms.

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“…Adipose tissue is abundant in the body and contains a stromal fraction rich in stem-progenitor cells capable of undergoing differentiation into osteogenic, chondrogenic, and adipogenic lineages [56] . The in vitro as well as in vivo properties of adipose tissue-derived stromal cells (ADSCs) resemble those of MSCs obtained from bone marrow, and the liposuction procedure employed to harvest ADSCs is minimally invasive for the patient [57] .…”

Section: Adipose Tissue-derived Mesenchymal Cellsmentioning

confidence: 99%

Mesenchymal stem cells in the treatment of spinal cord injuries: A review

Dasari

Veeravalli

Dinh

2014

WJSC

182

144

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With technological advances in basic research, the intricate mechanism of secondary delayed spinal cord injury (SCI) continues to unravel at a rapid pace. However, despite our deeper understanding of the molecular changes occurring after initial insult to the spinal cord, the cure for paralysis remains elusive. Current treatment of SCI is limited to early administration of high dose steroids to mitigate the harmful effect of cord edema that occurs after SCI and to reduce the cascade of secondary delayed SCI. R ecent evident-based clinical studies have cast doubt on the clinical benefit of steroids in SCI and intense focus on stem cell-based therapy has yielded some encouraging results. An array of mesenchymal stem cells (MSCs) from various sources with novel and promising strategies are being developed to improve function after SCI. In this review, we briefly discuss the pathophysiology of spinal cord injuries and characteristics and the potential sources of MSCs that can be used in the treatment of SCI. We will discuss the progress of MSCs application in research, focusing on the neuroprotective properties of MSCs. Finally, we will discuss the results from preclinical and clinical trials involving stem cell-based therapy in SCI. Core tip: Despite our deeper understanding of the molecular changes that occurs after the spinal cord injury (SCI), the cure for paralysis remains elusive. In this review, the pathophysiology of SCI and characteristics and potential sources of mesenchymal stem cells (MSCs) that can be used in the treatment of SCI were discussed. We also discussed the progress of application of MSCs in research focusing on the neuroprotective properties of MSCs. Finally, we discussed the results from preclinical and clinical trials involving stem cell-based therapy in SCI.

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Age-related changes of p75 Neurotrophin receptor-positive adipose-derived stem cells

Cited by 33 publications

References 42 publications

Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells

Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells

Transplantation of Predifferentiated Adipose-Derived Stromal Cells for the Treatment of Spinal Cord Injury

Mesenchymal stem cells in the treatment of spinal cord injuries: A review

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