Age-related changes of lipid fractions and total fatty acids in the serum of female and male rats aged from 37 to 1200 days

Murawski, U.; Kriesten, K.; Egge, Heinz

doi:10.1016/0305-0491(89)90192-2

Cited by 7 publications

(6 citation statements)

References 43 publications

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“…Lack of age-related differences in fed state in serum concentrations of glucose and FFA, main energy substrates, suggests an effi cient control of fuel metabolism during aging under physiological normal conditions. However, we and other authors observed increased serum triacylglycerides concentration, a characteristic feature of lipid metabolism alterations during aging both in rodents [10,11] , and man [12] . Enhanced accumulation of TG in adipose tissue and other organs is a key factor implicated in the increase of body weight in the elderly.…”

Section: Discussionmentioning

confidence: 83%

Effects of Fasting and Refeeding on Serum Leptin, Adiponectin and Free Fatty Acid Concentrations in Young and Old Male Rats

Kmieć¹,

Pokrywka²,

Kotlarz³

et al. 2005

Gerontology

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Background: Regulatory mechanisms of metabolic homeostasis undergo important alterations during ageing. The age-related changes become often evident only during stimulation of basic functions that occurs, e.g. during fasting and refeeding which represent natural challenge to energy metabolism. Objective: To determine the effect of short-term fasting and subsequent refeeding on serum levels of key hormones and metabolites in young adult 5- and 24-month-old male Wistar rats. Methods: Control rats were fed ad libitum. Animals were fasted for 48 h or fasted and refed for 24 h. Metabolite serum concentrations were measured by standard methods. Leptin and insulin were determined by rat-specific RIA, and adiponectin serum levels by mouse/rat–specific ELISA. Results: (1) Control serum levels of key metabolites and hormones were similar in both age groups except for increased triglycerides (TG) in old fed rats. (2) Fasting caused a significant decrease of leptin, insulin, glucose, and TG serum levels in both age groups, and an increase of free fatty acids (FFA) concentration, however, only in young animals. (3) Upon refeeding serum glucose, TG and insulin reversed to control levels in both age groups, however, FFA concentration decreased to control values only in young rats. (4) In contrast to young animals, refeeding of old rats did not increase serum leptin concentration to control level. (5) Neither fasting nor refeeding changed adiponectin serum levels in both age groups. Conclusion: Aging suppresses leptin secretion and metabolism of FFA during refeeding that follows short-term starvation. In old rats serum levels of FFA are refractive to the alterations induced by fasting/refeeding in young ones.

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Section: Discussionmentioning

confidence: 83%

Effects of Fasting and Refeeding on Serum Leptin, Adiponectin and Free Fatty Acid Concentrations in Young and Old Male Rats

Kmieć¹,

Pokrywka²,

Kotlarz³

et al. 2005

Gerontology

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“…Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were high in F344 rats at 30-56 weeks (Waner et al, 1991). Cholesterol and triglyceride (TG) synthesized or catabolized in the liver increase age-dependently in rats (Murawski et al, 1989;Ruggiero et al, 1990). Tsuchiya et al have reported that total protein (T.PRO), glucose (GLU), total cholesterol (T.CHO), TG, creatinine (CRE) and total bilirubin (T.BIL) were age-dependently increased and AST, alkaline phosphates (ALP) and inorganic phosphorus (IP) were age-dependently decreased in Sprague-Dawley rats (Tsuchiya et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

DNA DAMAGE MEASURED BY COMET ASSAY AND 8-OH-dG FORMATION RELATED TO BLOOD CHEMICAL ANALYSES IN AGED RATS

et al. 2007

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-To evaluate the effects of aging on DNA damage, spontaneous and chemical-induced DNA damage and its repair were examined using comet assays at pH 9, 12.1 and 13, and an 8-OH-dG assay in the liver and kidney of young (9-week-old) and aged (20-month-old) rats. Additionally, blood chemistry was examined to investigate any correlation between vital functions and age-dependent DNA damage. DNA migration at pH 13 and 8-OH-dG levels increased in the liver and/or kidney of aged rats, but DNA migration did not increase at pH 9 or 12.1; that is, alkali-labile sites and 8-OH-dG were concomitantly accumulated in aged rats. These results suggest that 8-OH-dG production caused by reactive oxygen species exceeded glycosylation and that the glycosylation activity is far more than the AP endonucleation in aged rats. Methyl methanesulfonate (MMS, 80 mg/kg, i.p.) increased DNA migration at pH 12.1 and 13 in the liver and kidney at 3 and 24 hr after treatment in young and aged rats. The DNA damage in aged rats was less and decreased more slowly compared with young rats. The pictures of MMSinduced DNA migrations at pH 12.1 and 13 were very similar to each other. These results suggest that the adduct glycosylation and repair of the single-strand breaks (SSBs) of aged rats are less than those of young rats, although AP endonucleation is sufficient to remove the AP sites. N-nitrosodiethylamine (160 mg/kg, i.p.) increased DNA migration at pH 12.1 and 13 in the liver and kidney at 3 and 24 hr in young rats and at pH 12.1 and 13 in the kidney at 24 hr in aged rats. These results showed that SSBs were predominantly detected as chemical-induced DNA damage and DNA repairs such as N-glycosylase, DNA polymerase and DNA ligase, and that the metabolic activation declined in aged rats. Aspartate aminotransferase, alanine aminotransferase, total bilirubin, total cholesterol, total protein, globulin, creatinine and chloride age-dependently increased and alkaline phosphates, albumin/globulin ratio, inorganic phosphorus and potassium age-dependently decreased, and these changes were correlated with the DNA migration at pH 13 and/or 8-OH-dG. These results suggest that the activity of DNA repair and metabolic activation enzymes declines in aged rats and that the accumulation of spontaneous DNA damage may affect vital functions.

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“…Free fatty acids displace a number of drugs from binding sites on albumin, 14,15 and circulating free fatty acids increase from young adulthood (3 months) through senescence in rats. 16,17 The purpose of the present study was to characterize and compare the in vitro protein binding of VPA in male Fischer-344 rats across age (14 days to 24 months of age) and to examine the influence of serum free fatty acid (FFA) and total protein (TP) concentrations on VPA protein binding during aging. The relationship between changes in FFA concentrations and binding of VPA to serum proteins in vitro also was investigated.…”

mentioning

confidence: 99%

Age-Related Changes in Valproic Acid Binding to Rat Serum Proteins in Vitro*

Slattum

Cato

Pollack

et al. 1996

Journal of Pharmaceutical Sciences

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The effect of age on the in vitro binding of valproic acid (VPA) to serum proteins was investigated in rats ranging in age from 14 days (preweaning) to 24 months (senescent). The influence of free fatty acid (FFA) and total protein (TP) concentrations on age-related changes in binding was examined. The protein binding of VPA was altered during development and aging. The VPA fraction unbound (fu) at low VPA concentrations was significantly higher in older age groups (12 and 24 months old; fu = 0.26-0.30) than in younger animals (14, 20, and 40 days old; fu = 0.16-0.18). Binding was best described by a model incorporating a saturable and a nonsaturable binding site. Binding affinity at the saturable binding site was lowest at the extremes of age. Changes in binding at either the saturable or the nonsaturable site were not predicted by changes in TP or FFA with age. Changes in nonsaturable binding were marginally associated with age (p = 0.0952). A 3-fold increase in FFA concentrations was necessary to produce a 1.5-fold increase in VPA fu. There was less than a 2-fold difference in FFA concentrations between the age groups (range 0.219-0.379 mmol/L). Thus, the difference in FFA concentrations between the age groups may not have been large enough to cause measurable differences in displacement of VPA from binding sites. Changes in protein binding may contribute to age-related changes in disposition of VPA observed in the rat. Changes in the serum concentrations of specific FFA or proteins may play a role in the altered VPA binding with age, but changes in total FFA or protein concentrations do not account for the age-related differences observed. Further investigation is required to identify the mechanism(s) responsible for age-related changes in binding of VPA to serum proteins measured in vitro.

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Age-related changes of lipid fractions and total fatty acids in the serum of female and male rats aged from 37 to 1200 days

Cited by 7 publications

References 43 publications

Effects of Fasting and Refeeding on Serum Leptin, Adiponectin and Free Fatty Acid Concentrations in Young and Old Male Rats

Effects of Fasting and Refeeding on Serum Leptin, Adiponectin and Free Fatty Acid Concentrations in Young and Old Male Rats

DNA DAMAGE MEASURED BY COMET ASSAY AND 8-OH-dG FORMATION RELATED TO BLOOD CHEMICAL ANALYSES IN AGED RATS

Age-Related Changes in Valproic Acid Binding to Rat Serum Proteins in Vitro*

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